Labshake search
Citations for Macherey-Nagel GmbH :
51 - 100 of 235 citations for Human Immunodeficiency Virus Reverse Transcriptase Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
-
bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
-
bioRxiv - Genetics 2022Quote: ... The protein concentration was determined by Protein Quantification Assay (Cat.# 740967,Macherey-Nagel). 10 µg of the protein was separated by SDS-PAGE electrophoresis ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was quantified using the Protein Quantification Assay kit (Macherey-Nagel). The protein samples were resolved using 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... All protein concentrations were determined with the MN Protein Quantification Assay (Macherey-Nagel). Western blotting was performed utilizing AnykD Mini-PROTEAN TGX Gels (Biorad ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein samples were extracted from the organs with the NucleoSpin RNA/Protein kit (Macherey-Nagel). Macherey-Nagel Bead Tubes Type F was used in tissue homogenization ...
-
bioRxiv - Genomics 2024Quote: ... RNA & Protein Purification kit (Macherey-Nagel). Clones were screened by XhoI digestion and Sanger sequencing.
-
bioRxiv - Cell Biology 2023Quote: ... and protein isolation kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel). RNA was further purified by using RNA Clean & Concentrator-25 kit (Zymo Research ...
-
bioRxiv - Genomics 2023Quote: ... reverse-crosslinked and subsequently purified through isopropanol and magnetic beads (Macherey-Nagel NucleoMag PCR Beads). Four-cutter restriction enzyme Csp6I (CviQI ...
-
bioRxiv - Immunology 2024Quote: ... and reverse transcription were performed using the NucleoSpin® RNA plus kit (Macherey-Nagel, 740984.50) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... Human keratinocyte RNA was purified using NucleoSpin RNA (Macherey-Nagel, Düren, Germany). Before the RNA preparation ...
-
bioRxiv - Genomics 2020Quote: Total protein was extracted from differentiated neurons at DIV 35 with the NucleoSpin RNA/Protein Kit (Macherey-Nagel 740933) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... genomic DNA was extracted from bacterial strains grown on King’s medium B (King et al. 1954) at 28°C for 24 h using NucleoSpin Microbial DNA kit (Macherey-Nagel, Germany). The quality of the genomic DNA was assessed by electrophoresis in 0.8% agarose gel.
-
bioRxiv - Cell Biology 2023Quote: ... Total RNA from human islets was extracted using Nucleospin RNA XS (Macherey-Nagel) three days post-transfection.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
-
bioRxiv - Genomics 2023Quote: ... and reverse-crosslinked DNA was subsequently purified using isopropanol and magnetic beads (Macherey-Nagel NucleoMag PCR Beads). Purified DNA was sheared to 300-500 bp with Covaris and subsequently size-selected by AMPure XP (Agencourt) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: RNA purification and reverse transcription were performed using the Nucleospin RNA kit from Macherey-Nagel (Düren, Germany) and the High capacity cDNA reverse transcription kit from Applied Biosystems (Woolston ...
-
bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel, Allentown, PA) (gene expression studies ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein and mRNA were extracted from the same leaf sample (NucleoSpin RNA/Protein kit, REF740933, Macherey-Nagel GmbH & Co., Düren, Germany).
-
bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reverse cross-linked chromatin was purified using the NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2020Quote: ... GRANTA-519 and B-cells purified from four MCL patients using the NucleoSpin® RNA II kit according to the manufacturer’s protocol (Macherey-Nagel, Oensingen, Switzerland). A minimum of 2µg per samples were used for analysis ...
-
bioRxiv - Biochemistry 2021Quote: ... Cleaved protein was passed through Ni-TED (Macherey-Nagel) to remove uncleaved protein ...
-
bioRxiv - Biochemistry 2024Quote: All proteins were purified using Ni-IDA (Macherey-Nagel) following the instructions provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
-
bioRxiv - Microbiology 2024Quote: ... Total RNA for quantitative reverse transcription PCR (qRT-PCR) was isolated using the NucleoSpin® RNA Kit (Macherey-Nagel), including DNA digestion ...
-
bioRxiv - Immunology 2021Quote: ... total protein was isolated by NucleoSpin TriPrep kit (Macherey-Nagel) from 5×106 PMNs ...
-
jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: Total RNA from human cell lines were extracted using NucleoSpin RNA Plus columns (Macherey-Nagel, France), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: Total RNAs from cultured human iPS cells were extracted using NucleoSpin RNA Plus XS (Macherey-Nagel). The 100 ng of total RNAs were used as starting materials to generate RNA-seq libraries with the TruSeq Stranded mRNA LT sample prep kit (Illumina) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The drug concentration in samples was measured with high-performance liquid chromatography (HPLC) using a reverse phase C18 Nucleosil 100-5 (4.0 × 250 mm; 5.0 μm particle size, Macherey-Nagel, Germany) column and mobile phase consisting of MeOH/water (90/10 v/v ...
-
bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
-
bioRxiv - Biochemistry 2022Quote: ... Amino acids were separated on a reverse-phase C18 column (EC 250/2 NUCLEOSIL 100-3 C18HD, L=250 mm, ID: 2 mm; Macherey-Nagel). Samples (5 µL ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer 5’AACCCAGGTAATGAACACAGTTTCTAT. PCR products were run on a gel (supplemental Fig. 2B) and bands were gel purified (Macherey-Nagel), inserted in a pCR-bluntII-Topo vector (Invitrogen ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins were batch-purified using Ni-NTA agarose resin (Macherey-Nagel) and eluted using the abovementioned lysis buffer with increased imidazole concentration (250 mM) ...
-
bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using Protino NTA columns (Macherey-Nagel, Freemansburg, PA, USA) using linear imidazole gradients (30-250 mM) ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
-
bioRxiv - Immunology 2022Quote: ... The protein was purified on a Ni-NTA resin column (Macherey-Nagel) and the eluted fractions were concentrated and dialyzed against PBS using the same Amicon system as described above ...
-
bioRxiv - Cell Biology 2019Quote: mRNA was isolated using the NucleoSpin® RNA/Protein kit (Macherey-Nagel). Purified mRNA was transcribed into cDNA with the QuantiTect®Reverse Transcription Kit (Qiagen) ...
-
bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
-
bioRxiv - Cell Biology 2023Quote: ... The proteins were then transferred onto Porablot NCP nitrocellulose membrane (Macherey-Nagel) and blocked for 1 hour at RT in 3% BSA (Applichem ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from six human fecal samples using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany), as described previously [36] ...
-
bioRxiv - Plant Biology 2024Quote: ... The protein was purified using Protino Ni-NTA agarose (Macherey-Nagel, #7450400-500).
-
bioRxiv - Biochemistry 2023Quote: ... The GST fusion proteins were purified with Protino Glutathione Agarose 4B (Macherey-Nagel) and GST was cleaved by human thrombin (Biopharm laboratories ...
-
bioRxiv - Biochemistry 2021Quote: ... Both peptide segments were purified by C18 reverse phase (RP)-HPLC on a Nucleosil C18 column (300 Å, 250 × 10 mm) (Macherey-Nagel, Düren, Germany), maintained at 25°C ...
-
bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were captured using glutathione-agarose column (Protino glutathione-agarose 4B, Macherey-Nagel Gmbh) equilibrated with 20 mM Tris HCl ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extractions were performed with NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France) according to the manufacturer’s instructions ...