Labshake search
Citations for Macherey-Nagel GmbH :
51 - 100 of 204 citations for 7 Bromo 2 3 4 5 tetrahydro 1H benzo e 1 4 diazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
-
bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
-
bioRxiv - Cancer Biology 2023Quote: RNA was isolated from Kasumi-1 cells after 2 days after shRUNX1::ETO knockdown was induced with doxycycline using the Nucleospin RNA kit (Macherey-Nagel). cDNA was synthesised using Superscript II (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: ... an EC 100/2 Nucleoshell Phenyl-Hexyl column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
-
bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated for 1 hour with 2 g Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany). The matrix was washed extensively with buffer L and bound protein was eluted in buffer L containing 250 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
-
bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
-
bioRxiv - Physiology 2022Quote: ... Japan) equipped with an analytical CN-RP column (NUCLEODUR 100-5, CN-RP, 150 × 4.6, 5 µm column; Macherey-Nagel GmbH & Co. KG, Düren, Germany) and a UV-diode-array detector ...
-
bioRxiv - Plant Biology 2020Quote: ... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...
-
bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
-
bioRxiv - Genetics 2021Quote: ... PCR of genomic DNA extracted 3 days after nucleofection using NucleoSpin Tissue kit (Macherey-Nagel) was performed using specific primers and Sanger sequenced (Stabvida ...
-
bioRxiv - Molecular Biology 2023Quote: ... The reaction mixture was diluted 5-fold with the NTC buffer (Macherey-Nagel), and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
-
1H NMR chemical exchange techniques reveal local and global effects of oxidized cytosine derivativesbioRxiv - Biophysics 2021Quote: ... As stationary phase Nucleodur columns (250/10 mm, C18ec, 5 μm) from Macherey-Nagel were used ...
-
bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel). One-Step RT-PCR SuperScript™ III Platinum™ Kit (Life Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was loaded onto a 5 mL Ni-NTA column (Macherey-Nagel). After washing with a buffer consisting of 20 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... followed by rapid filtration through membrane filters (MN gf-5 0.4 μm; Macherey-Nagel). The filters were dissolved in 5 ml of scintillation fluid (MP Biomedicals) ...
-
bioRxiv - Plant Biology 2023Quote: ... thaliana 5-days-old seedlings was isolated using NucleoSpinTM RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: Total RNA was extracted from ovaries of 3-day-old females using the NucleoSpin RNA isolation kit (Macherey-Nagel), following the instructions of the manufacturer ...
-
bioRxiv - Biochemistry 2021Quote: ... with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel, Düren, Germany) running a general gradient of 10% to 70% acetonitrile (ACN ...
-
bioRxiv - Cell Biology 2022Quote: ... and EBs at day 5 using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germany) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... with the difference that a Nucleodur 100-5 NH2 125x4 mm column (Macherey-Nagel, #760730.40) and an eluent consisting of 8.5% acetonitrile and 25mM formic acid pH 4 were used (18) ...
-
bioRxiv - Developmental Biology 2024Quote: ... the cleared lysate was loaded onto a 5 mL Protino GST/4B column (Macherey-Nagel) and washed with 10 column volumes of lysis buffer ...
-
bioRxiv - Genetics 2019Quote: Total RNA was extracted from ovaries of 3-day-old females using the NucleoSpin® RNA isolation kit (Macherey-Nagel), following the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2021Quote: ... as well as cKD TgISU1 (incubated with or without ATc at 1.5 μg/mL for 3 days) were extracted using Nucleospin RNA II Kit (Macherey-Nagel). The cDNAs were synthesized with 450 ng of total RNA per RT-PCR reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2021Quote: Genomic DNA was isolated from 5 Mio HeLa cells using the NucleoSpin tissue kit (Macherey-Nagel) according to the manufacturers protocol.
-
bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from mESCs and MEFs (3 biological replicates of WT and Atxn7l3−/− for each cell types) using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: The genomic DNA was extracted from at least one million cells (typically 3 million, coverage 300x) using the NucleoSpin Tissue kit (Macherey-Nagel). To enrich for GBCs ...
-
bioRxiv - Microbiology 2023Quote: ... and 300 mg (JFC) of both fresh and treated frass (n = 3) using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) and following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 5×106 B16F10 tumor cell lines using NucleoSpin® Tissue (MACHEREY-NAGEL), according to manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The filters were then individually placed into 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel), added with 1,200 µL MWA1 lysis buffer and stored at - 80°C until nucleic acids extraction as specified in the corresponding section below.
-
bioRxiv - Biochemistry 2021Quote: ... equipped with a flame ionization detector (FID) and an Optima-s-3 GC capillary column (30 m x 0.25 mm x 0.25 µm - Macherey-Nagel GmbH & Co, Germany). Nitrogen (200 kPa ...
-
bioRxiv - Evolutionary Biology 2020Quote: Fifteen microliters of extract were injected into an Agilent 1100 series HPLC and compounds were separated on an EC 150/4.6 NUCLEODUR® C18 Gravity column (3 μm, 150 mm x 4.6 mm, Macherey-Nagel, Düren, Germany). Cardenolides and bufadienolides were eluted at a constant flow of 0.7 ml/min at 30°C with an acetonitrile–H2O gradient as follows ...
-
bioRxiv - Genetics 2021Quote: Genomic DNA from 5 males was extracted using the NucleoSpin Tissue XS kit (Macherey-Nagel, ref #740 901). To amplify the Rsp satDNA region ...
-
bioRxiv - Plant Biology 2021Quote: ... dissolved and fractionated by RP-HPLC using a Nucleodur 100-5 C18ec 125×4.6 mm column (Macherey-Nagel). The ABA-containing fraction was collected ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM PMSF and purified by immobilized metal affinity chromatography using a 5 ml nickel column (Macherey-Nagel). The protein was eluted from the nickel column with solubilization buffer supplemented with 500 mM imidazole ...
-
bioRxiv - Synthetic Biology 2023Quote: ... preparative HPLC was employed using a reversed-phase C18 column (Macherey-Nagel VP 250/21 Nucleodur 100-5) with a mobile phase solvent A (Water + 0.05% vol/vol trifluoroacetic acid (TFA)) ...
-
bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
-
bioRxiv - Genomics 2020Quote: Total mRNA of edited HUDEP-2 cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse transcribed into cDNA ...
-
bioRxiv - Developmental Biology 2022Quote: ... The supernatant was collected and loaded onto 2 mL pre-equilibrated Ni-NTA agarose (Macherey-Nagel). The mixture was rotated at 4 □ for 2 h ...
-
bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
-
bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
-
bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... For Clr purification the initial purification was conducted using a 5 mL Protino™ Ni-NTA Column (Macherey-Nagel). Size-exclusion chromatography over a 16/600 Superdex 200 column (GE ...
-
bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
-
bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
-
bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
-
bioRxiv - Microbiology 2023Quote: Separation was performed on an EC 100/2 Nucleoshell Phenyl-Hexyl column (2C×C100Cmm, 2.7Cμm; Macherey-Nagel) at 40°C ...
-
bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...