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Citations for Macherey-Nagel GmbH :
751 - 800 of 2234 citations for Plant DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we removed the last segments of the caudal section and preserved it in 96% ethanol before DNA extraction using the NucleoSpin® 96 Tissue kit (Macherey-Nagel). It is worth noting that the ablation of the last segments of the caudal section of earthworms does not affect their survival due to their regeneration capacity (e.g ...
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bioRxiv - Microbiology 2021Quote: ... mitochondrial and nuclear genomes we extracted genomic DNA from Pb-GFP infected hepatocytes treated or not with infusion using a NuceolSpin Tissue kit (Macherey-Nagel, Germany) according to manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA genes of AOB and AOA was amplified with primers amoA-1F/amoA-2R (66 ...
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bioRxiv - Microbiology 2022Quote: ... Viral genome equivalents (vge) were determined by qPCR of DNA isolated from encapsidated virions isolated with NucleoSpin Blood kit QuickPure (Macherey-Nagel; 740569.250).
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bioRxiv - Cancer Biology 2019Quote: Cells pellet (1.5*10^6) were collected at different time points after LPS stimulation and DNA was extracted with the Nucleospin tissue kit (Macherey-Nagel, 740952). The genomic DNA was eluted in 50 μl of BE buffer (5 mM Tris/HCl ...
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bioRxiv - Zoology 2020Quote: Approximately 50-100 mg of tissue (from the second pereopod) was used for DNA extraction using the NucleoSpin® Tissue Kit (Macherey-Nagel) following the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: Viral biodistribution analysis - Genomic DNA (gDNA) was extracted from the brain region of interest with the NucleoSpin®Tissue Kit (Macherey-Nagel) extraction kit and 100 ng of gDNA was used as a qPCR template ...
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bioRxiv - Microbiology 2019Quote: ... All single fragment PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Overlap PCRs were carried out using 100 ng of each purified PCR products and the resulting fragment of interest was purified from agarose gel ...
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bioRxiv - Genomics 2021Quote: Sequencing libraries were prepared from the cells collected at the end of the screens as follows: Genomic DNA was extracted using the NucleoSpin® Blood XL kit (Macherey-Nagel) with a condition of 50e6-100e6 cells per column ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 mL co-culture taken from different time points during the cultivation experiment using the NucleoSpin Tissue DNA extraction kit according to the manufacturer’s instructions (Macherey-Nagel, Düren, Germany). Cell numbers were quantified by direct epifluorescence microscopy of Sybr-green stained cells immobilized on agarose-coated slides to generate standard curves for qPCR as described previously [42] ...
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bioRxiv - Genomics 2019Quote: ... CHP-100 and HS-SYII) and tissue (ES1 and RH) was extracted by using the column-based NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The overlap PCR product was purified from agarose gel and was directly used to transform wild type F ...
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bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The resulting powder was used for DNA extraction using the NucleoSpin® Tissue kit following manufacturer instructions (MACHEREY-NAGEL GmbH & Co. KG). DNA quantity and purity were checked with a Nanodrop One spectrophotometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were grown in Luria-Bertani Broth at 37°C and plasmid DNA was isolated using the Nucleobond Xtra midi kit (MACHEREY-NAGEL, 740410), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA gene of Ca ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cultured alone C4-2B cells or the mixed co-cultured C4-2B/MC3T3-E1 were harvested for DNA extraction using the NucleoSpin Tissue kit (Macherey-Nagel Inc.). Primers against the nuclear and mitochondrial genomes were used for qRT-PCR (sequences provided in Supplementary Table S2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The isolation of gDNA was conducted either by using a column-based method following manufacturer’s instructions (NucleoSpin Microbial DNA kit, Macherey-Nagel, Düren, Germany) or an automated system (King Fisher Duo Prime System ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was purified according to the manufacturer’s instructions (NucleoBond HMW DNA, Macherey-Nagel). A total of 280ug of genomic DNAs was used for PCR amplification of sgRNA sequences ...
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bioRxiv - Microbiology 2021Quote: ... was linearized by AdeI restriction enzyme digestion and DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). For in-vitro transcription ...
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bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
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bioRxiv - Microbiology 2019Quote: ... The strain was grown overnight in de Man-Rogosa-Sharpe (MRS) medium (Carl Roth, Karlsruhe, Germany) and DNA was extracted using the NucleoSpin 96 tissue kit (Macherey-Nagel, Düren, Germany), with an extra cell lysis step using 20 mg/mL of lysozyme (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: DNA extraction was performed from a single female 4th instar larva (ToLID ilPecGoss1) using the NucleoMag Tissue kit (Macherey-Nagel, Allentown, PA, USA). The high molecular weight DNA was prepared for PacBio sequencing using the SMRTbell Express Template Prep Kit 2.0 for Continuous Long Read (CLR ...
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bioRxiv - Microbiology 2022Quote: ... The total genomic DNA of the insect individual and its symbionts was extracted using the NucleoSpin Tissue XS Kit (MACHEREY-NAGEL, Duren, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 8×104 661W cells were seeded in a 24-well culture plate with DMEM and transfected with the plasmids containing guides using Lipofectamine™ Cells were harvested 72 hours following the transfection and Genomic DNA was then extracted using NucleoSpin® Tissue kit (Macherey-Nagel) following the manufacturer’s recommendation.
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bioRxiv - Molecular Biology 2023Quote: ... 500 µg of ground mycelium was used for extraction of high molecular weight DNA with the NucleoBond HMW-Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... If no genomic DNA could be obtained strains were cultured according to the manufacturer’s protocol and DNA was extracted using the NucleosSpin Plasmid (NoLid) Mini kit (Macherey-Nagel cat. No. 740499) with vortexing after addition of BufferA2 and BufferA3 ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... infected cells were washed five times to eliminate non-internalized VPs and DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel #740952-250). Internalized vector genomes were titrated by qPCR using a forward primer in the U exon ...
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bioRxiv - Microbiology 2023Quote: Whole peripheral blood was collected from newly hatched chicks and total DNA was extracted using the NucleoSpin 96 Blood core kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Systems Biology 2020Quote: ... with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Plasmids were constructed from PCR-generated fragments and pET16b vector cut with restriction enzymes using the isothermal DNA assembly method (48) ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Zoology 2024Quote: ... Genomic (g)DNA was extracted and purified using the NucleoSpin®Tissue kit following the manufacturer’s instructions (Macherey-Nagel Gmbh & Co. KG, Düren, Germany). Primers used for the detection of all pathogens are found in Supplementary Data 3.
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bioRxiv - Synthetic Biology 2023Quote: ... Base-editing efficiency when targeting a plasmid-borne gene was estimated by isolating plasmid DNA upon 24-h editing treatment by using the NucleoSpin™ plasmid EasyPure kit (Macherey-Nagel, Dueren, Germany). Either the isolated plasmids or the purified PCR products from independent samples were Sanger-sequenced using the Mix2Seq™ kit (Eurofins Scientific ...
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bioRxiv - Genomics 2020Quote: ... DNA extraction (Nucleospin Tissue, Macherey-Nagel) was performed on the resulting pellet according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Released DNA was purified (Macherey-Nagel ™ NucleoSpin ™ gel and PCR cleaning kit ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated (Macherey-Nagel, 740420.50) and the sequences were checked with sequencing (LGC Genomics GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... products stemming from the TdT-labeling of oligonucleotide TdT3 were irradiated with UV light prior and after purification with a NucleoSpin (Macherey-Nagel) clean-up kit.
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 12,000 ×g at 4 °C for 20 minutes and the cleared supernatant was either directly used for the reconstitution assays or subjected to purification with Ni-NTA agarose (Macherey-Nagel). The suspension was incubated for 60 minutes and transferred to a fretted column ...
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bioRxiv - Immunology 2020Quote: Following stimulation cells were lysed and total RNA isolated using Nucleospin RNA purification columns according to the manufactures protocol (Macherey-Nagel). 0.5 to 1 µg of total RNA was reverse transcribed using BioRad iScript ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The digestion reaction was run on a 1% agarose gel followed by excision of the digested band and purification via NucleoSpin columns (Macherey-Nagel). 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB ...