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Citations for Macherey-Nagel GmbH :
601 - 650 of 802 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Total RNAs were extracted from blood of 137 ewes with the Nucleospin® RNA Blood Kit (Macherey-Nagel, #Ref 740200.50) according to the manufacturer’s protocol starting with 800µL of whole blood with a DNAseI digestion treatment to eliminate contaminating genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 200 mg of stool sample using a modified NucleoSpin® DNA Stool kit (Macherey-Nagel, Germany) via physical ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from the final pellets using the NucleoBond High Molecular Weight DNA Kit according to the manufacturer’s instruction (Macherey-Nagel).
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted from larvae or pupae of the selected lines using NucleoSpin Tissue gDNA extraction kit (Macherey-Nagel).
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bioRxiv - Bioengineering 2023Quote: We extracted RNA from live cells for bulk RNA-sequencing using the NucleoSpin totalRNA FFPE micro kit (Macherey-Nagel #740969.50) ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was extracted from the heads of 30 male flies using the NucleoZol one phase RNA purification kit (Macherey-Nagel). Library preparation and sequencing was performed by BGI (Wuhan ...
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bioRxiv - Neuroscience 2023Quote: ... A fused-silica capillary column Optima 17 (15 m length, 0.25 mm I.D., 0.25 µm film thickness) from Macherey-Nagel (Düren, Germany) was used ...
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bioRxiv - Genomics 2023Quote: ... high-molecular weight genomic DNA was extracted from the leaves of R511 using NucleoBond HMW DNA (MACHEREY-NAGEL, Dueren, Germany). Genomic DNA was prepared using the SMRTbell Express Template Prep Kit (PacBio ...
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bioRxiv - Biophysics 2022Quote: ... Ligated DNA constructs were separated with agarose gels from other reaction products and purified by gel extraction (NucleoSpin, Macherey-Nagel) by avoiding any exposure to ethidium bromide or UV light.
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the cultured cells using the Nucleospin RNA Plus kit (cat. 740984, Macherey-Nagel, CA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA from BMDMs and RAW 264.7 cells was isolated using the NucleoSpin RNA II kit (Macherey-Nagel, Catalog #740955). The cDNA of pre-mRNA was synthesized by the use of random hexamer primers (Thermo-Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... RNA from FAC-sorted lung EC was extracted using the Nucleaospin® TriPrep Kit (MACHEREY-NAGEL, 740966.50, Düren, Germany Germany). RNA quantity and quality were assessed using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested in RIPA with 1X HALT or the RNA lysis buffer from the Nucleospin RNA kit (Macherey-Nagel).
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
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bioRxiv - Biochemistry 2023Quote: ... The thin layer chromatography was done with silica gel on aluminum foils (60 Å pore diameter) obtained from Macherey-Nagel and visualized with ultraviolet light (λ = 254 and 365 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... Products from four PCR reactions were pooled and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel #740609). Equal amounts of products were mixed and sequenced on Illumina NextSeq 500 instruments using the NextSeq high-output kit (75 cycles ...
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bioRxiv - Microbiology 2024Quote: ... Microbial DNA was obtained from 200 mg of rhizosphere material using the Nucleo Spin® Soil kit (Macherey-Nagel, Germany). Quantitative determination of the concentration (ng/μl ...
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bioRxiv - Genetics 2024Quote: ... We purified the resulting fragment from a 2% agarose gel using the NucleoSpin Gel and PCR Purification Kit (Macherey-Nagel). We amplified the 1483bp RTFDC1 promoter sequence off of a promoter plasmid (Genecopoeia HPRM41362-PF02 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA from tissues or cells was isolated using an RNA isolation kit (NucleoSpin RNA, Macherey-Nagel GmbH & co.KG, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from the spleen of 4-6 weeks-old asymptomatic mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNAs were extracted from about 6 ×106 HepaRG cells using a Nucleospin RNA isolation Kit (Macherey-Nagel, Hoert, France) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from two or three legs per specimen using a NucleoSpin® Tissue kit (MACHEREY-NAGEL Düren, Germany). The isolated DNA was resuspended in Tris-HCl buffer (pH 7.5 ...
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A comparative analysis between two flax varieties indicates lignan-mediated salt stress adaptivenessbioRxiv - Plant Biology 2024Quote: Total RNA was isolated from 100 mg of shoot sample using the NucleoSpin RNA Plant mini kit (Macherey-Nagel, Germany) following the kit-specified protocol ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from the thymi of 8 weeks-old asymptomatic male mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We grew plants in the greenhouse and extracted DNA from young leaf tissue using NucleoSpin Plant II kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... DNA from three female and three male fifth instar larvae was isolated individually using the NucleoSpin DNA Insect kit (Macherey-Nagel). We observed that the primers Masc_F1 and Masc_R1 targeting EkMasc and EkMascB consistently showed off-target amplification in female samples ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from flowers and whole chili pepper fruits at different developmental stages using a NucleoSpin(tm) RNA Plant kit (MACHEREY-NAGEL) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... products stemming from the TdT-labeling of oligonucleotide TdT3 were irradiated with UV light prior and after purification with a NucleoSpin (Macherey-Nagel) clean-up kit.
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bioRxiv - Developmental Biology 2020Quote: ... DNA from a male wild type blood sample (2 ml) was purified with the NucleoBand®HMW DNA Kit (Macherey-Nagel). To eliminate fragments below 40 kb the Short Read Elimination Kit XL (Circulomics ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The products were isolated by cutting the band from an agarose gel and clean-up with the NucleoSpin Extract II kit (Macherey-Nagel). Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... Large quantities of plasmid DNAs for transfection were prepared from the pellets of overnight cultures of pNC-HN8 and pNC-HN8GGm clones using the NucleoBond Xtra Midi kit (Macherey-Nagel) as instructed by the manufacturer.
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bioRxiv - Cell Biology 2021Quote: ... In the following steps each amplicon generated either by PCR amplification or vector digestion was isolated and extracted from a 1% agarose gel through the NucleoSpin Gel (Macherey-Nagel) and PCR Clean-Up Kit (Takara ...
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bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from leaves of four-week-old Arabidopsis plants was extracted using the NucleoSpin RNA plant extraction kit (Macherey-Nagel) and treated with rDNase according to the manufacturer’s extraction protocol ...
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bioRxiv - Plant Biology 2022Quote: 100mg of leaf tissues from the second to fourth fully emerged leaf from the apical bud were used for DNA extraction using the NucleoSpin Plant II kit (Macherey-Nagel). Leaf tissues were disrupted with 400µL PL1 from the kit using BeadBeater (BioSpec ...
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bioRxiv - Plant Biology 2022Quote: 30mg of leaf tissues from the eighth fully emerged leaf from the apical bud were used for RNA extraction using the NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel). Leaf samples were sampled from the plants that were at the end of 8 weeks after being transplanted to the 2-gallon pot ...
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bioRxiv - Microbiology 2022Quote: ... the band corresponding to the amplicon was excised from the gel and purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA from each sample was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel, Hoerdt Cedex, France) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the genomic DNA from the edited cells was isolated after the transduction for eight days using a DNA isolation kit (NucleoSpinBlood - Macherey-Nagel). The target region for base editing was amplified using the respective primers (Sup Tables 2 and 3) ...
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bioRxiv - Plant Biology 2021Quote: Ribonucleic acid (RNA) samples were isolated from seeds and leaves using the NucleoSpin® RNA Plant kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Viral RNA was isolated from 4 µl of a typical lentivirus preparation (∼2×106 TU/µl) using the NucleoSpin RNA Virus mini kit (Macherey-Nagel). Purified RNA was used as a template for reverse transcription using the SuperScript VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... DNA fragments were purified by agarose gel electrophoresis followed by extraction from an excised gel slice using a NucleoSpin Extract II kit (Macherey-Nagel). Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit ...
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...