Labshake search
Citations for Macherey-Nagel GmbH :
551 - 600 of 713 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... All PCR fragments for cloning and plasmids were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and GenElute™ Plasmid Miniprep Kit (Merck) ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
-
bioRxiv - Molecular Biology 2022Quote: ... Certain PCR products were purified from the gel using NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) and cloned individually in a TA-cloning vector called pGEM®-T Easy Vector (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... Five tubes of each amplicon were pooled and DNA purified using NucleoSpin Gel and PCR Clean-Up (Macherey-Nagel). DNA was eluted in 20 µl of Elution Buffer and concentration was measured using Qubit 3.0 fluorometer (Life Technologies).
-
bioRxiv - Bioengineering 2022Quote: ... All constructs were verified by sequencing and purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
-
bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
-
bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2024Quote: ... the bands of interest were excised and purified with the Gel and PCR clean up (ref 740609, Macherey-Nagel) with a final elution volume of 20 µl ...
-
bioRxiv - Molecular Biology 2024Quote: ... The sample was then acidified using trifluoroacetic acid and peptides were purified using Chromabond C18 Microspin columns (Macherey-Nagel).
-
bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel Cat#740609.50), and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX) ...
-
bioRxiv - Microbiology 2024Quote: ... All positive samples were purified with the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). Briefly ...
-
bioRxiv - Microbiology 2024Quote: ... Each mix was then purified using the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). DNA was then eluted and quantified at a Nanodrop 2000 Spectrophotometer (ThermoScientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... PCR products were purified to remove unincorporated nucleotides and primers using NucleoFast® 96 PCR plates (Macherey-Nagel, Oensingen, Switzerland). Details of the annealing temperatures and primers were published previously (Friesen et al ...
-
bioRxiv - Molecular Biology 2019Quote: ... the PCR product was purified with NucleoSpin Gel and PCR clean-up kit according to manufacturer’s description (Macherey-Nagel, 740609). RF cloning into pET28a was performed with eighteen 3-step cycles with annealing temperature 65°C and extension time 7 minutes ...
-
bioRxiv - Genomics 2019Quote: ... PCR products were checked by agarose gel electrophoresis and purified using Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Cycle sequencing reactions were performed using the BigDye 3.1 chemistry ...
-
bioRxiv - Microbiology 2019Quote: ... PCR products were then purified using a Millipore NucleoFast 96 PCR kit following the manufacturer’s recommendations (Macherey-Nagel, Düren, Germany) and sequenced using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems ...
-
bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
-
bioRxiv - Microbiology 2022Quote: ... and purified directly from the PCR mix or from an agarose gel (Gel and PCR clean-up kit, Macherey-Nagel); the two fragments were fused together thanks to a primer-encoded 20-30bps overlap during a second PCR amplification round and the product cloned into the pEMG suicide vector [20] using EcoRI and BamHI ...
-
bioRxiv - Molecular Biology 2020Quote: ... 50 μL water was added and DNA was purified using Nucleospin Gel and PCR Cleanup (Macherey-Nagel, Cat. No 740609.250). Extracted DNA was PCR-amplified to check the insertion of the 3XFLAG tag ...
-
bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
-
bioRxiv - Cancer Biology 2019Quote: ... guide RNAs and Cas9 were expressed from plasmids that were purified with the NucleoBond® Xtra Midi Kit (Macherey-Nagel). In the remaining mixes ...
-
bioRxiv - Immunology 2020Quote: ... Positive clones were identified by colony PCR and the plasmid was purified using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel). The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
-
bioRxiv - Immunology 2020Quote: ... amplicons were separated by gel electrophoresis and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Library quantification and quality control was performed using Qubit (QIAGEN ...
-
bioRxiv - Molecular Biology 2021Quote: ... His6-BsrA was purified from the cell lysate by chromatography on Ni-agarose columns (Protino Ni-TED 1000, Macherey-Nagel) with 300 mM imidazole in phosphate buffer used for elution ...
-
bioRxiv - Plant Biology 2019Quote: ... DNA from the IP and input fractions was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). DNA from IP and input were used for library preparation and sequencing by Illumina Hi-Seq (FASTERIS ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were recovered and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, DE). Concentrations of purified PCR products were determined by absorbance measurements at a wavelength of 260 nm using an infinite F200 pro device (TECAN ...
-
bioRxiv - Developmental Biology 2021Quote: ... The DNAs have been extracted on an agarose gel and purified (using the PCR clean-up gel extraction kit #740609.50, Macherey-Nagel) and the sanger sequencings have been performed by Eurofins ...
-
bioRxiv - Genetics 2020Quote: ... Obtained libraries were pooled in equimolar amounts and purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), followed by a second purification step of gel extraction and size exclusion with AMPure XP magnetic beads (Beckman Coulter) ...
-
bioRxiv - Biophysics 2022Quote: ... The product was then purified from 1% agarose gel and extracted using the NucleoSpin clean up kit (Macherey-Nagel, 740588.50).
-
bioRxiv - Microbiology 2022Quote: ... The amplicons were purified by the NucleoSpin Gel and PCR cleanup kit according to manufacturer’s instructions (Macherey-Nagel, Dueren, Germany). Purified products were sent for Sanger sequencing with primers rbcL404+ and rbcL587-to Macrogen (http://dna.macrogen-europe.com).
-
bioRxiv - Plant Biology 2023Quote: ... Total DNA was extracted using CTAB (Doyle & Doyle 1987) and purified using NucleoSpin® Purification Kit (Macherey-Nagel, Düren, Germany). DNA extractions were profiled in Agarose gel 1% and using Nanodrop ...
-
bioRxiv - Synthetic Biology 2022Quote: ... SLS was precipitated with trifluoroacetic acid (TFA, 1.5% final concentration) and peptides were purified using Chromabond C18 microspin columns (Macherey-Nagel). Acidified peptides were loaded on spin columns equilibrated with 400 μL acetonitrile and then 400 μL 0.15% TFA ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The 140-bp UPTAG and DNTAG libraries were purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel), quantified using the Qubit dsDNA HS kit (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
-
bioRxiv - Biophysics 2022Quote: ... Ligated DNA constructs were separated with agarose gels from other reaction products and purified by gel extraction (NucleoSpin, Macherey-Nagel) by avoiding any exposure to ethidium bromide or UV light.
-
bioRxiv - Immunology 2023Quote: Cells from a confluent 6-well were lysed and RNA was purified using the NucleoSpin RNA Plus kit for RNA purification (740984.50, Macherey-Nagel) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: cDNA constructs for mammalian tissue culture were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (no. 740410.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
-
bioRxiv - Cell Biology 2023Quote: ... The pooled extracts were further desalted and purified on a Chromabond® C18 ec poplyprolyene column (Macherey-Nagel, Düren, Germany). The extracted glycolipids were evaporated to dryness under nitrogen gas to remove organic solvents for deglycosylation ...
-
bioRxiv - Biochemistry 2023Quote: ... The PCR products were purified using the NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, catalog no. 740609.50) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed on 1% agarose gel and purified by NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel). 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... for 45 min at 65°C and purified using NucleoBond AXG20 Mini columns according to the user manual (Macherey-Nagel). Final high molecular weight gDNA was quantified (Qubit) ...
-
bioRxiv - Plant Biology 2023Quote: ... 6His-AtREM1.21–118 was produced like GST-CPK and purified on Protino® Ni-TED column following manufacturer’s instructions (Macherey-Nagel). After elution with 40-250 mM imidazole ...
-
bioRxiv - Biochemistry 2024Quote: ... pcDNA5/FRT-SUGCT and pOG44 plasmid DNA was purified by NucleoBond Xtra Midi Plus EF DNA purification Kit (Macherey-Nagel). Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: ... The CBP-His6-ZZ-Upf1-HD proteins were first purified on a Protino® Ni-NTA agarose resin (Macherey-Nagel). After successive washing steps using buffers C200 first ...
-
bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...