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Citations for Tonbo Biosciences :
1 - 50 of 51 citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the ELISA kits to determine the levels of IFNγ and TNFα from house mouse (Mus muscuslus) (Tonbo Bioscience, San Diego, CA) were utilized to detect those cytokines in white-footed mice ...
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bioRxiv - Immunology 2023Quote: ... 500 μL single-cell suspensions were incubated at 37 °C for 6 h with 5% CO2 in 48-well plates in the presence of Cell Stimulation Cocktail (Tonbo Biosciences, #TNB-4975). Stimulated cells were stained with surface markers as described above ...
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bioRxiv - Immunology 2023Quote: ... IL-10 ELISA matched antibodies were obtained from Tonbo Biosciences (San Diego, CA, USA) and the ELISA was carried out according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD4 APC (Tonbo Biosciences; clone RM4-5) and CD8 PE (Tonbo Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Cells were activated with plate-bound anti-CD3 (Tonbo Biosciences #40-0038) at a concentration of 10 µg/mL and anti-CD28 (Tonbo Biosciences #40-0289 ...
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bioRxiv - Immunology 2020Quote: ... recombinant IL-4 and IL-5 (10 ng/mL; Tonbo Bioscience) cytokines ...
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bioRxiv - Immunology 2021Quote: ... CD4 (PerCP/BV605, Tonbo Bioscience/Biolegend, 1:200, clone RM4-5), CD8a (APC-Cy7/APC-H7/APC ...
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bioRxiv - Immunology 2023Quote: ... CD4 (PerCP/BV605, Tonbo Bioscience/Biolegend, 1:200, clone RM4-5), CD8α (APC-Cy7/APC-H7/APC ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences). Cells were stimulated for 48 hours prior to electroporation.
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bioRxiv - Immunology 2021Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences) at a final concentration of 2.5 × 106 cells/ml ...
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bioRxiv - Microbiology 2024Quote: ... Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml; CD28.2; Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C and 5% CO2 before treatment with KL-2.
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bioRxiv - Immunology 2024Quote: ... using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT, TONBO Biosciences) was performed ...
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bioRxiv - Microbiology 2020Quote: ... Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2, Tonbo Biosciences). Cells were stimulated for 72 h at 37°C in a 5%-CO2 atmosphere prior to electroporation ...
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bioRxiv - Cancer Biology 2022Quote: ... kit (Tonbo Biosciences, 20-1172), and EpCAM (BioLegend ...
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bioRxiv - Microbiology 2019Quote: ... and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences, clone CD28.2; #70-0289-U100). For each electroporation 1 × 106 CD4+ T cells were pelleted by centrifugation at 100 x g for 10 mins and washed once in PBS ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2020Quote: ... The PBMCs were cultured in three different ways: 1) Plate-bound anti-CD3 + anti-CD28 (clones OKT3 and CD28.2 at 2μg/ml each - Tonbo Biosciences) - plates were coated with Goat anti-mouse IgG (clone Poly4053 at 10μg/ml - BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... cells were labelled with 5 µM carboxyfluorescein succinimidyl ester for 8 min at (CFSE; Tonbo Biosciences) for 8 min at 37°C and seeded at progressively reduced density (no less than 3000 cells/cm2) ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Microbiology 2019Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3 coated plates [coated for 2 hours at 37°C with 20µg/mL anti-CD3 (UCHT1, Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... were incubated overnight at 4°C with PBS containing plate-bound anti-CD3χ (4 μg/ml, 145-2C11, Tonbo Biosciences) and anti-CD28 (4 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (TNB-0607-KIT, Tonbo Biosciences, San Diego, CA, USA), and stained with antibodies against intracellular proteins ...
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bioRxiv - Microbiology 2020Quote: ... These cells were immediately stimulated on anti-CD3-coated plates (coated overnight with 10 μg/ml anti-CD3 from Tonbo Biosciences) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Microbiology 2020Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3-coated plates [coated for 12 hours at 4°C with 20 µg/ml anti-CD3 antibody (UCHT1, Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2 ...
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bioRxiv - Microbiology 2024Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3–coated plates coated for 2 hours at 37°C with anti-CD3 (20 mg/ml) (UCHT1; Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... a transcription factor-staining kit (Tonbo Biosciences) was used ...
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bioRxiv - Immunology 2020Quote: ... Transcription Factor Staining Buffer Kit (Tonbo Biosciences) was used to fix and permeabilize cells before treating cells with Fc Block and subsequent intracellular staining ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed with a FoxP3 Fix/Perm kit (eBioscience) or FoxP3/transcription factor staining buffer kit (Tonbo Biosciences). These kits were also used for permeabilization prior to staining with intracellular antibodies ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
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bioRxiv - Immunology 2020Quote: ... followed by fixation and permeabilization with a FoxP3 Fix/Perm kit (eBioscience) or FoxP3/transcription factor staining buffer kit (Tonbo Biosciences) and staining with intracellular antibodies.
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bioRxiv - Immunology 2022Quote: Single cell suspensions were enumerated 5×106 cells stimulated with 1X of cell stimulation cocktail (Tonbo Biosciences, San Diego CA) for 5 hours ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Immunology 2023Quote: 96-well round bottom plates were coated with (0, 1, 10 μg/mL) anti-CD3ε (clone# 17A2, Ref# 40-0032, Tonbo Biosciences, San Diego, CA) or PBS for 2 hours at 37°C and removed by aspiration ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer’s protocol then plated in 96-well round bottom plates and cultured in the presence of anti-CD28 (0, 4, 10 μg/mL, Ref# 40-0281, Tonbo Biosciences, San Diego, CA) and/ or capsaicin ...
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bioRxiv - Immunology 2023Quote: ... the FoxP3 / Transcription Factor Staining Buffer kit (Tonbo Biosciences) and protocol was followed ...
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bioRxiv - Immunology 2023Quote: ... the Foxp3/Transcription Factor Staining Buffer Kit (Tonbo Biosciences) was used ...
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bioRxiv - Cell Biology 2021Quote: ... cells were immediately fixed and permeabilized using a transcription factor staining kit according to the manufacturer protocol (Tonbo Biosciences Cat: TNB-0607-KIT). After fixation and permeabilization cells were spun down ...
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bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized for 30 min at room temperature using a FoxP3 / Transcription Factor Staining Buffer Kit (Tonbo Biosciences; cat. # TNB-0607-KIT). A panel of the following mAbs was used for the longitudinal phenotyping of innate immune cells in whole blood (500 μL) ...
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bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized for 30 min at room temperature using a FoxP3 / Transcription Factor Staining Buffer Kit (Tonbo Biosciences; cat. # TNB-0607-KIT). A panel of the following mAbs was used for the longitudinal phenotyping of innate immune cells in whole blood (500 μL) ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... 140 mM NaCl) and stained at room temperature with 1 µg/ml DAPI and 5 µl of Annexin V FITC (Tonbo Biosciences, San Diego, CA) for 15 minutes in the dark ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed and permeabilized (Transcription Factor Staining Buffer Kit, Tonbo Biosciences) and labeled with E16-APC for 20min at room temperature (56) ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed and permeabilized (Transcription Factor Staining Buffer Kit, Tonbo Biosciences) and labeled with E16-APC for 20min at room temperature (16) ...
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bioRxiv - Immunology 2021Quote: ... Tonbo Bioscience FoxP3 Fix/Perm kit was used per manufacturer’s instructions (Tonbo Bioscience, San Diego, California)
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bioRxiv - Developmental Biology 2023Quote: ... TUNEL assays were performed using APO-Direct Kit (TNB-6611-R, Tonbo Biosciences, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... or fixed overnight at 4°C for intracellular staining the next day using a FOXP3 kit (Tonbo Biosciences). The staining was performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Permeabilization and intracellular staining steps were performed with the FoxP3 permeabilization buffer kit (Tonbo Biosciences, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Samples were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Kit (Tonbo Biosciences, San Diego, CA, U.S.), then incubated with primary-conjugated antibodies for 45 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with the Foxp3 Transcription factor staining Buffer kit according to manufacturer’s recommendations (Tonbo Biosciences), followed by intracellular staining with anti-Foxp3-PE (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and resuspended in Transcription Factor Fix/Perm solution prepared as per manufacturer’s protocol (TNB-0607-KIT; Tonbo Biosciences). Following 30 mins incubation at RT ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with 1x Flow Cytometry Perm Buffer prepared as per manufacturer’s instructions (TNB-0607-KIT; Tonbo Biosciences) before staining with intracellular marker antibodies for 30 mins at 4°C in the dark (Table S2).