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Citations for Tonbo Biosciences :
1 - 50 of 223 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and 1% penicillin-streptomycin-fungizone [PSF]) or basal media supplemented with 10 ng/mL of IL-1β (human recombinant; Tonbo Bioscience 218018U010) for 2 weeks ...
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bioRxiv - Immunology 2022Quote: ... and recombinant Mouse IL-15 (Tonbo Biosciences) were suspended and mixed in PBS ...
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bioRxiv - Microbiology 2019Quote: ... We obtained our recombinant proteins from Tonbo biosciences which included mouse CXCL1 ...
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bioRxiv - Immunology 2024Quote: ... APCCy7 anti-human/mouse CD11b (clone M1/70, Tonbo Biosciences, 1:2000), PECy7 anti-mouse CD11c (clone N418 ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience) plus 20 ng/mL recombinant human BAFF (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... and recombinant IL-4 cytokine (10 ng/mL; Tonbo Bioscience) with or without SCD inhibitor SSI-4 ...
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bioRxiv - Immunology 2020Quote: ... recombinant IL-4 and IL-5 (10 ng/mL; Tonbo Bioscience) cytokines ...
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bioRxiv - Developmental Biology 2022Quote: Lung cells were plated at 1×106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-human CD4 (Tonbo Biosciences, 20-0048-T025), and sorted for live 7-AAD- CD3+ CD4+ T-lymphocytes using the BDFACS ARIA II with a 100 uM nozzle ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... BM cell suspensions were lysed in 1 mL of red blood cell lysis buffer (Tonbo Biosciences, San Diego, CA), washed with 1X phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions were stained with 1:1000 Ghost dye violet 510 dead cell stain for 405nm excitation (Tonbo biosciences) in PBS for 30 min followed by a wash with 2% NCS HBSS ...
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bioRxiv - Developmental Biology 2023Quote: Lungs were plated at 1 × 106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Cancer Biology 2019Quote: ... staining with APC Anti-Human CD3 (clone OKT3, 20-0037-T100, Tonbo Biosciences), monitoring GFP ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were washed thoroughly and stained for dead cell exclusion (Ghost Violet 510, 1:1000 dilution Tonbo Biosciences, San Diego, CA) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in Fc block (1:100, Tonbo Bioscience, 70-0161-U500) followed by indicated antibodies and fixed with 1% PFA in flow cytometry buffer ...
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bioRxiv - Microbiology 2020Quote: HeLa cells were pretreated with 500 U ml−1 IFN-β (Tonbo Biosciences 21-8699) or vehicle (0.1% BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells from BALF were stained with CD16/32 (Tonbo biosciences 70-0161-u500, 1:10) to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were collected after 45 min incubation at 37 °C and 5 % CO2 and stained with antibodies to microglia cell surface markers (CD11b-PE-Cy7, 1:200, Biolegend, #101216; CD45-APC-Cy7, 1:200, Tonbo Biosciences, #25-0459-T100). XO4+ and XO4− microglia uptake of pHrodo-conjugated synaptosomes or pHrodo™ Green E ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded by a fixable viability dye (Ghost Red 710, Tonbo Biosciences, 1:6400). The following antibodies were used for flow cytometry:
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bioRxiv - Cell Biology 2024Quote: ... The cells were then stained using live/dead dye (1:1000, Ghost Dye Red 780, TONBO biosciences for myeloid ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent antibodies ...
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bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Immunology 2023Quote: Freshly thawed villous leukocytes (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...
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bioRxiv - Microbiology 2023Quote: ... About 500,000 cells were stained as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 510 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... CD45+ lymphocytes were flow sorted and 1 x 106 cells were used for staining with antibody cocktail (Supplementary Table 4) with anti-mouse CD16/32 (TONBO biosciences, 40-0161) for 30 minutes at room temperature in a final volume of 100uL in MaxPAR cell staining buffer (Fluidigm ...
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bioRxiv - Immunology 2021Quote: ... Cells were surface stained in an additional 50 uL FACS containing 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent anti-human antibodies ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were enumerated 5×106 cells stimulated with 1X of cell stimulation cocktail (Tonbo Biosciences, San Diego CA) for 5 hours ...
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bioRxiv - Immunology 2021Quote: ... PD-1 (PE-Cy7, Tonbo Biosciences, 1:200, clone J43.1), PD-L1 (PE/BV711/PE-Cy7 ...
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bioRxiv - Immunology 2023Quote: ... PD-1 (PE-Cy7, Tonbo Biosciences, 1:200, clone J43.1), PD- L1 (PE/BV711 ...
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bioRxiv - Immunology 2022Quote: ... Collected lavage was spun down and cells were blocked with CD16/32 prior being stained with Ly6G PE-Cy7 (Tonbo Biosciences, 60-1276-U100, clone 1A8, 1:200) and F4/80 APC (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were excluded using Ghost DyeTM Violet 450 cell viability dye from Tonbo Biosciences. For intracellular staining of active caspase-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... NK-92 cells were collected and restimulated with cell stimulation cocktail (#TNB-4975-UL100, Tonbo Biosciences) for 4 h and intracellular staining was performed ...
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bioRxiv - Microbiology 2021Quote: ... while cell stimulation cocktail (Tonbo Biosciences) or medium alone conditions were used as positive and negative controls respectively ...
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bioRxiv - Immunology 2019Quote: ... frozen cells were thawed and stained for live cells using Ghost-Dye 710 (Tonbo Biosciences, CA, USA). 50,000 lived cells were FACS sorted and washed once with cold PBS ...
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bioRxiv - Immunology 2021Quote: ... Splenic cells underwent two rounds of red blood cell lysing using 1X RBC lysis buffer (Tonbo Biosciences). Cells were washed in PBS ...
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bioRxiv - Immunology 2020Quote: ... and the red blood cells were removed by inclubating the cells with RBC lysis buffer (Tonbo Biosciences). Atfer that ...
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bioRxiv - Cancer Biology 2022Quote: ... Gr-1 (Tonbo Biosciences, 60-5931), F4/80 (Tonbo Biosciences ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: PE anti-mouse FcεRI (clone MAR-1, Tonbo Biosciences, 1:500), FITC anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Cancer Biology 2022Quote: ... The myeloid panel contained GhostDye (1:1000) and antibodies directed against CD45 (Tonbo Biosciences #50-0451; 1:250), CD11b (Tonbo Biosciences #75-0112 ...
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bioRxiv - Immunology 2020Quote: ... cells suspensions were stimulated for 4 hours ex vivo using a commercial cell stimulation cocktail (Tonbo Biosciences, catalog no. TNB-4975) prior to staining for flow cytometry as above ...
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bioRxiv - Cancer Biology 2022Quote: ... CD11c (Tonbo Biosciences #60-0114; 1:250), F4/80 (Tonbo Biosciences #20-4801 ...
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bioRxiv - Cancer Biology 2022Quote: ... Ly6G (Tonbo Biosciences #25-1276; 1:250), and Ly6C (Biolegend #128049 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD45 (Tonbo Biosciences #50-0451; 1:250), and CD8 (Tonbo Biosciences #20-1886 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD11b (Tonbo Biosciences #75-0112; 1:250), CD11c (Tonbo Biosciences #60-0114 ...