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Citations for Tonbo Biosciences :
51 - 88 of 88 citations for Recombinant Human CD19 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The APC-conjugated anti-FoxP3 mAb was purchased from Tonbo Biosciences (San Diego, CA). The FITC-conjugated mouse lineage marker cocktail (CD3 ...
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bioRxiv - Immunology 2024Quote: ... followed by intranuclear staining with an anti-Foxp3-APC Ab (clone; 3G3, TONBO Bioscience) using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were blocked in αCD16/32 mouse Fc block (Tonbo Biosciences #70-0161) and stained for extracellular proteins using an antibody master mix made in buffer ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 1 μg Fc Block (anti-CD16/32, Tonbo Biosciences) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: Staining panels for mouse included anti-Fc (anti-CD16/CD32) (Tonbo Biosciences, 2.4G2), anti-CD11b (Tonbo Biosciences ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... prior to Fc-block incubation (anti-mouse CD16/CD32, clone 2.4G2, Tonbo Biosciences). CD8+ T cells were purified by negative selection using biotinylated antibodies and magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... Cell staining was conducted in the presence of Fc Block (2.4G2, Tonbo Biosciences) at 4°C for 30 minutes for all surface markers except CXCR5-biotin which was stained at room temperature for 45 minutes ...
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bioRxiv - Immunology 2022Quote: Ex vivo and cultured PBMC were stained with APC-H7 (Tonbo Biosciences, San Diego, CA) or Brilliant Violet 405 (Molecular Probes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in Fc block (1:100, Tonbo Bioscience, 70-0161-U500) followed by indicated antibodies and fixed with 1% PFA in flow cytometry buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... a Fc receptor blocker (Tonbo Biosciences 70-0161-M001, Tonbo Biosciences, San Diego, CA) was added for 10 minutes at 4°C to prevent non-specific binding of antibodies ...
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bioRxiv - Immunology 2022Quote: Analysis of cell viability ex vivo or after culture was performed using APC-H7 (Tonbo Biosciences) or LIVE/DEA Fixable Yellow 405 (invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse CD8α-APC antibody (0.7 μL; cat. #20-1886, Clone 2.43, Tonbo Biosciences, San Diego, CA) was added then incubated for 10 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... myeloid DCs were quantified by gating for CD11+ cells (APC, clone 3,9 TONBO bioscience 20-0116-T100) and pDCs were quantified by gating for CD303+ cells (PE-Cy7 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse CD16/32 antibody (2.4G2) used for Fc receptor blocking was obtained from Tonbo Biosciences. Unconjugated mouse and rat IgG antibodies were purchase from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Immunology 2024Quote: DAPI (#11034-56, Nacalai Tescue) and Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences) were added to the cells just before staining with specific antibodies against cell surface molecules ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in the study are as follows: APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences), Alexa Fluor 700 anti-mouse CD45 (Clone ...
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bioRxiv - Immunology 2023Quote: ... Additional antibodies used for this experiment included anti-IFNψ-APC (Tonbo Biosciences Cat# 20-7311 (also OWL-A01038), RRID:AB_2621616) ...
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bioRxiv - Pathology 2024Quote: ... dead cells were first labeled with Ghost Dye Red 780 (13-0865-T500, Tonbo Biosciences) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse CD16/CD32 Fc Block antibody purchased at a concentration of 2mg/mL (Tonbo Biosciences 40-0161-M001) was added at 0.5 uL per sample (in 100uL total volume per sample of block antibody diluted in PBS ...
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bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for 20 minutes at RT with mouse PE conjugated 5-OP-RU MR1-tetramer (NIH Tetramer core) and the following antibodies: anti-CD4-FITC/APC-Fire810 (clone GK1.5, Tonbo Biosciences/Biolegend), anti-CD8α-AF700 (clone 53-6.7 ...
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bioRxiv - Microbiology 2021Quote: ... samples were washed with PBS pH7.2 (Fisher Cat#20012027) and incubated with Fc Block (Tonbo Biosciences Cat# 70-0161-U500) for 5 minutes on ice before viability staining (BD Biosciences Cat# 564406) ...
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bioRxiv - Developmental Biology 2023Quote: Lungs were plated at 1 × 106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-human CD4 (Tonbo Biosciences, 20-0048-T025), and sorted for live 7-AAD- CD3+ CD4+ T-lymphocytes using the BDFACS ARIA II with a 100 uM nozzle ...
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bioRxiv - Developmental Biology 2022Quote: Lung cells were plated at 1×106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were collected after 45 min incubation at 37 °C and 5 % CO2 and stained with antibodies to microglia cell surface markers (CD11b-PE-Cy7, 1:200, Biolegend, #101216; CD45-APC-Cy7, 1:200, Tonbo Biosciences, #25-0459-T100). XO4+ and XO4− microglia uptake of pHrodo-conjugated synaptosomes or pHrodo™ Green E ...
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bioRxiv - Immunology 2024Quote: ... APCCy7 anti-human/mouse CD11b (clone M1/70, Tonbo Biosciences, 1:2000), PECy7 anti-mouse CD11c (clone N418 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... 0.025% NaN3) and Fcγ receptors were blocked with 1/200 diluted anti-mouse CD16/32 antibodies (Fc block) (Tonbo Biosciences, San Diego, CA, USA) for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
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bioRxiv - Immunology 2024Quote: Cells were incubated with anti-mouse primary antibodies to assess immune populations with the following conjugated antibodies: B220-APC (Tonbo Biosciences, San Diego, CA; clone RA3-6B2), CD19-PE/Dazzle594 (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies against mouse proteins were used: anti-CD16/CD32 (2.4G2, TONBO Bioscience), anti-CD45 (30-F11 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular protein and cytokine staining was performed using the Foxp3/Transcription Factor Staining Buffer Set (Tonbo Biosciences). Mouse and rat anti-human monoclonal antibodies (mAbs ...