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Citations for Tonbo Biosciences :
1 - 50 of 57 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and IL-4 (10 ng/mL; Tonbo Bioscience), or TLR ligand CpG (2.5 μM ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience) plus 20 ng/mL recombinant human BAFF (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... and recombinant IL-4 cytokine (10 ng/mL; Tonbo Bioscience) with or without SCD inhibitor SSI-4 ...
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bioRxiv - Immunology 2020Quote: ... recombinant IL-4 and IL-5 (10 ng/mL; Tonbo Bioscience) cytokines ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% penicillin-streptomycin-fungizone [PSF]) or basal media supplemented with 10 ng/mL of IL-1β (human recombinant; Tonbo Bioscience 218018U010) for 2 weeks ...
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bioRxiv - Immunology 2024Quote: ... APCCy7 anti-human/mouse CD11b (clone M1/70, Tonbo Biosciences, 1:2000), PECy7 anti-mouse CD11c (clone N418 ...
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bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
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bioRxiv - Immunology 2022Quote: Cells were stained in blocking solution with a Live/Dead marker (Ghost Dye Violet510, Tonbo Biosciences) for 30 minutes at 4ºC ...
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bioRxiv - Bioengineering 2023Quote: ... 10ng/mL IL-15 (Tonbo Biosciences, 218157U002), and 10ng/mL IL-7 (Tonbo Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Each sample was stained with 10 μL propidium iodide (PI) staining solution (Tonbo Biosciences, cat# 13-6990-T200) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected from 1.0 × 106 lung cells/mL media restimulated overnight with plate-bound anti-CD3 (adsorbed to wells at 1.0 μg/ml in PBS) and soluble anti-CD28 (1.0 μg/ml) (Tonbo Biosciences), as previously described (36 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... and 10ng/mL IL-7 (Tonbo Biosciences, 218079U002). Stimulated PBMCs were electroporated using the Neon transfection system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and resuspended in Transcription Factor Fix/Perm solution prepared as per manufacturer’s protocol (TNB-0607-KIT; Tonbo Biosciences). Following 30 mins incubation at RT ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL anti-CD28 (CD28.2, Tonbo Biosciences). CD4 T cells were purified as described above and 3 x 105 CD4 T cells were pelleted in a V-bottom plate and resuspended in 20 µL P3 buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.5μg/ml 7AAD (13-6993-T200, Tonbo Biosciences) in 2.5 mM Ca2+-containing RPMI for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and brain-derived neurotrophic factor (BDNF; Tonbo Biosciences, 1μg/ml), with 50% media replacement every second day ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 μg/mL anti-mouse CD16/32 (TONBO Biosciences) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg/ml anti-mouse CD16/CD32 (2.4G2) (Tonbo Biosciences), surface antibodies and Fixable Viability Dye eFluor 780 (eBioscience) ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg/ml anti-mouse CD16/CD32 (2.4G2) (Tonbo Biosciences), surface antibodies and Fixable Viability Dye eFluor 780 (eBioscience) ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2, Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C / 5% CO2 prior to electroporation.
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bioRxiv - Molecular Biology 2020Quote: ... fixation and permeabilization was performed using a 1X solution of Foxp3/Transcription Factor Fix/Perm Concentrate (4X) (Tonbo Biosciences, San Diego, CA; Cat#TNB-1020-L050) that was diluted using the 1X Foxp3/Transcription Factor Fix/Perm Diluent (Tonbo Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were suspended in 0.5μg/ml 7-AAD (13-6993-T500, Tonbo Biosciences) in 1mL staining solution [900μL H2O + 100μL NaCitrate (380mM)] for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences). Cells were stimulated for 48 hours prior to electroporation.
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bioRxiv - Cancer Biology 2019Quote: ... or E4 (10ug/ml; Tonbo Biosciences 21-9195, 21-9189, and 21-9190) were added to the media ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences) at a final concentration of 2.5 × 106 cells/ml ...
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bioRxiv - Microbiology 2024Quote: ... Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml; CD28.2; Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C and 5% CO2 before treatment with KL-2.
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bioRxiv - Microbiology 2020Quote: ... Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2, Tonbo Biosciences). Cells were stimulated for 72 h at 37°C in a 5%-CO2 atmosphere prior to electroporation ...
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bioRxiv - Microbiology 2023Quote: ... at a concentration of 10 µg/mL and anti-CD28 (Tonbo Biosciences #40-0289) supplemented to the media at a concentration of 5 µg/mL.
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bioRxiv - Microbiology 2019Quote: ... and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences, clone CD28.2; #70-0289-U100). For each electroporation 1 × 106 CD4+ T cells were pelleted by centrifugation at 100 x g for 10 mins and washed once in PBS ...
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bioRxiv - Microbiology 2020Quote: HeLa cells were pretreated with 500 U ml−1 IFN-β (Tonbo Biosciences 21-8699) or vehicle (0.1% BSA ...
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bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2020Quote: ... The PBMCs were cultured in three different ways: 1) Plate-bound anti-CD3 + anti-CD28 (clones OKT3 and CD28.2 at 2μg/ml each - Tonbo Biosciences) - plates were coated with Goat anti-mouse IgG (clone Poly4053 at 10μg/ml - BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... BM cell suspensions were lysed in 1 mL of red blood cell lysis buffer (Tonbo Biosciences, San Diego, CA), washed with 1X phosphate-buffered saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse CD16/CD32 Fc Block antibody purchased at a concentration of 2mg/mL (Tonbo Biosciences 40-0161-M001) was added at 0.5 uL per sample (in 100uL total volume per sample of block antibody diluted in PBS ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Microbiology 2019Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3 coated plates [coated for 2 hours at 37°C with 20µg/mL anti-CD3 (UCHT1, Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... after which 5×104 APCs and 1×105 T cells were co-cultured for 14-18 hours in the presence of 2μg/mL anti-CD28 (40-0281-M001, Tonbo Biosciences). T cell IFNγ production was detected via biotinylated IFNγ detection antibody (551881 ...
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bioRxiv - Bioengineering 2023Quote: ... Debrided samples were then stained in 1.5 mL 1 × phosphate buffered saline (PBS) with 1:500 Ghost Dye 780 (Tonbo Biosciences) and 3 drops NucBlue (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... were incubated overnight at 4°C with PBS containing plate-bound anti-CD3χ (4 μg/ml, 145-2C11, Tonbo Biosciences) and anti-CD28 (4 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... These cells were immediately stimulated on anti-CD3-coated plates (coated overnight with 10 μg/ml anti-CD3 from Tonbo Biosciences) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were stimulated with 1 nM SIINFEKL (OT-I CD8+ T cells) or in wells coated with anti-CD3 (10 μg/ml; clone: 145-2C11; Tonbo Biosciences) and anti-CD28 (2 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Bone marrow cells were isolated from wild-type C57BL/6 mouse femurs and differentiated to bone marrow-derived macrophages (BMDM) using murine GM-CSF (50ng/mL, Tonbo Biosciences) in DMEM (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3-coated plates [coated for 12 hours at 4°C with 20 µg/ml anti-CD3 antibody (UCHT1, Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2 ...
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bioRxiv - Microbiology 2023Quote: ... 20 x 106 splenocytes were processed and stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Up to 20 x 106 lung cells were stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...