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Citations for Tonbo Biosciences :
1 - 50 of 51 citations for Human Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Microbiology 2020Quote: ... the ELISA kits to determine the levels of IFNγ and TNFα from house mouse (Mus muscuslus) (Tonbo Bioscience, San Diego, CA) were utilized to detect those cytokines in white-footed mice ...
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bioRxiv - Immunology 2023Quote: ... IL-10 ELISA matched antibodies were obtained from Tonbo Biosciences (San Diego, CA, USA) and the ELISA was carried out according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-human CD4 (Tonbo Biosciences, 20-0048-T025), and sorted for live 7-AAD- CD3+ CD4+ T-lymphocytes using the BDFACS ARIA II with a 100 uM nozzle ...
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bioRxiv - Immunology 2024Quote: ... APCCy7 anti-human/mouse CD11b (clone M1/70, Tonbo Biosciences, 1:2000), PECy7 anti-mouse CD11c (clone N418 ...
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bioRxiv - Cancer Biology 2019Quote: ... staining with APC Anti-Human CD3 (clone OKT3, 20-0037-T100, Tonbo Biosciences), monitoring GFP ...
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bioRxiv - Microbiology 2019Quote: ... We obtained our recombinant proteins from Tonbo biosciences which included mouse CXCL1 ...
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bioRxiv - Microbiology 2021Quote: ... and IL-2-APC (Tonbo bioscience, 20-7021) on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...
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bioRxiv - Immunology 2024Quote: ... using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT, TONBO Biosciences) was performed ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies against mouse proteins were used: anti-CD16/CD32 (2.4G2, TONBO Bioscience), anti-CD45 (30-F11 ...
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bioRxiv - Cancer Biology 2022Quote: ... kit (Tonbo Biosciences, 20-1172), and EpCAM (BioLegend ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% penicillin-streptomycin-fungizone [PSF]) or basal media supplemented with 10 ng/mL of IL-1β (human recombinant; Tonbo Bioscience 218018U010) for 2 weeks ...
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bioRxiv - Immunology 2023Quote: ... permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (TNB-0607-KIT, Tonbo Biosciences, San Diego, CA, USA), and stained with antibodies against intracellular proteins ...
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bioRxiv - Immunology 2022Quote: ... Intracellular protein and cytokine staining was performed using the Foxp3/Transcription Factor Staining Buffer Set (Tonbo Biosciences). Mouse and rat anti-human monoclonal antibodies (mAbs ...
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bioRxiv - Immunology 2020Quote: ... a transcription factor-staining kit (Tonbo Biosciences) was used ...
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bioRxiv - Immunology 2020Quote: ... Transcription Factor Staining Buffer Kit (Tonbo Biosciences) was used to fix and permeabilize cells before treating cells with Fc Block and subsequent intracellular staining ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed with a FoxP3 Fix/Perm kit (eBioscience) or FoxP3/transcription factor staining buffer kit (Tonbo Biosciences). These kits were also used for permeabilization prior to staining with intracellular antibodies ...
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bioRxiv - Immunology 2020Quote: Mice were intravenously (i.v.) injected with 2 µg of α-CD45 (Clone: 30-F11) (Tonbo Biosciences) diluted in 300 µL of sterile PBS five minutes before sacrifice ...
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bioRxiv - Immunology 2020Quote: ... followed by fixation and permeabilization with a FoxP3 Fix/Perm kit (eBioscience) or FoxP3/transcription factor staining buffer kit (Tonbo Biosciences) and staining with intracellular antibodies.
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bioRxiv - Immunology 2023Quote: ... the FoxP3 / Transcription Factor Staining Buffer kit (Tonbo Biosciences) and protocol was followed ...
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bioRxiv - Immunology 2023Quote: ... the Foxp3/Transcription Factor Staining Buffer Kit (Tonbo Biosciences) was used ...
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bioRxiv - Immunology 2023Quote: ... mice were intravenously injected by retro-orbital injection with 2 μg α-CD45-FITC (30-F11, Tonbo Biosciences) antibody to detect remaining circulating cells in lung samples (52) ...
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bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Cell Biology 2021Quote: ... cells were immediately fixed and permeabilized using a transcription factor staining kit according to the manufacturer protocol (Tonbo Biosciences Cat: TNB-0607-KIT). After fixation and permeabilization cells were spun down ...
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bioRxiv - Immunology 2023Quote: Freshly thawed villous leukocytes (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized for 30 min at room temperature using a FoxP3 / Transcription Factor Staining Buffer Kit (Tonbo Biosciences; cat. # TNB-0607-KIT). A panel of the following mAbs was used for the longitudinal phenotyping of innate immune cells in whole blood (500 μL) ...
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bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized for 30 min at room temperature using a FoxP3 / Transcription Factor Staining Buffer Kit (Tonbo Biosciences; cat. # TNB-0607-KIT). A panel of the following mAbs was used for the longitudinal phenotyping of innate immune cells in whole blood (500 μL) ...
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bioRxiv - Microbiology 2019Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3 coated plates [coated for 2 hours at 37°C with 20µg/mL anti-CD3 (UCHT1, Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged and resuspended in FACS buffer (PBS supplemented with 2% FBS) and stained with Ghost Dye Violet 510 (1:1000, Tonbo Biosciences) on ice for 10 min for discrimination of viable and non-viable cells ...
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bioRxiv - Microbiology 2024Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3–coated plates coated for 2 hours at 37°C with anti-CD3 (20 mg/ml) (UCHT1; Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed and permeabilized (Transcription Factor Staining Buffer Kit, Tonbo Biosciences) and labeled with E16-APC for 20min at room temperature (56) ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed and permeabilized (Transcription Factor Staining Buffer Kit, Tonbo Biosciences) and labeled with E16-APC for 20min at room temperature (16) ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
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bioRxiv - Immunology 2022Quote: Single-cell suspensions were washed with FACS buffer (1X PBS/2% FBS/2mM EDTA/0.05% sodium azide) and stained with ghost dye red 780 viability dye (Tonbo Biosciences 13-0865-T100) and antibodies against TCRβ (H57-597 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 naive DPB1*04:01 positive donors’ PBMCs were stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13- 0870-T100) and 1 µL of DPB1*04:01-S167-180-tetramer ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... Tonbo Bioscience FoxP3 Fix/Perm kit was used per manufacturer’s instructions (Tonbo Bioscience, San Diego, California)
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bioRxiv - Developmental Biology 2023Quote: ... TUNEL assays were performed using APO-Direct Kit (TNB-6611-R, Tonbo Biosciences, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... or fixed overnight at 4°C for intracellular staining the next day using a FOXP3 kit (Tonbo Biosciences). The staining was performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Permeabilization and intracellular staining steps were performed with the FoxP3 permeabilization buffer kit (Tonbo Biosciences, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Samples were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Kit (Tonbo Biosciences, San Diego, CA, U.S.), then incubated with primary-conjugated antibodies for 45 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with the Foxp3 Transcription factor staining Buffer kit according to manufacturer’s recommendations (Tonbo Biosciences), followed by intracellular staining with anti-Foxp3-PE (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and resuspended in Transcription Factor Fix/Perm solution prepared as per manufacturer’s protocol (TNB-0607-KIT; Tonbo Biosciences). Following 30 mins incubation at RT ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with 1x Flow Cytometry Perm Buffer prepared as per manufacturer’s instructions (TNB-0607-KIT; Tonbo Biosciences) before staining with intracellular marker antibodies for 30 mins at 4°C in the dark (Table S2).