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Citations for Tonbo Biosciences :
51 - 100 of 102 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences, CA), and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE ...
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bioRxiv - Microbiology 2023Quote: ... 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences, CA), and 20 minute incubation with primary antibody cocktail (CD45+ immune cells ...
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bioRxiv - Microbiology 2023Quote: ... 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences, CA), and 20 minute incubation with primary antibody cocktail (Ly6eΔCD4 ...
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bioRxiv - Immunology 2023Quote: ... PD- L1 (PE/BV711, Tonbo Biosciences/Biolegend, 1:100, clone 10F.9G2), CD44 (AF700 ...
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bioRxiv - Cancer Biology 2022Quote: ... as well as GhostDye viability dye (Tonbo Biosciences #13-0865; 1:1000). The myeloid panel contained GhostDye (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 μL of Ghost-viability dye (Tonbo Biosciences; 13-0870-T100) in 1 mL PBS ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 PE-Cy7 (clone GL-1, Tonbo Biosciences, San Diego, CA), and anti-mouse F4/80 BUV395 (clone T45-2342 ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 1 μg Fc Block (anti-CD16/32, Tonbo Biosciences) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... as indicated: Ghost viability dye (1:400; Tonbo Biosciences Cat. 13-0870-T100); CD4-AF488 (1:300 ...
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bioRxiv - Immunology 2021Quote: ... CD3e (PE-Cy5/AF532, Tonbo Bioscience/Thermo Fischer, 1:200, clone 145-2C11), CD4 (PerCP/BV605 ...
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bioRxiv - Immunology 2023Quote: ... CD3e (PE-Cy5/AF532, Tonbo Bioscience/Thermo Fischer, 1:200, clone 145-2C11), CD4 (PerCP/BV605 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in Fc block (1:100, Tonbo Bioscience, 70-0161-U500) followed by indicated antibodies and fixed with 1% PFA in flow cytometry buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 µg total RNA was used to cDNA synthesis (TONBO Biosciences, 31-5300-0100R). qRT-PCR studies were performed using TONBO Biosciences CYBER Fast™ qPCR Hi-ROX Master Mix ...
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bioRxiv - Immunology 2021Quote: ... PD-L1 (PE/BV711/PE-Cy7, Tonbo Biosciences/Biolegend, 1:100, clone 10F.9G2), CD44 (AF700 ...
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bioRxiv - Immunology 2021Quote: ... Intranuclear staining for Tcf-1 was performed using a Foxp3 staining buffer set (Tonbo bioscience) and stained with anti-Tcf-1 (C63D9 ...
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bioRxiv - Microbiology 2020Quote: HeLa cells were pretreated with 500 U ml−1 IFN-β (Tonbo Biosciences 21-8699) or vehicle (0.1% BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells from BALF were stained with CD16/32 (Tonbo biosciences 70-0161-u500, 1:10) to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was used to synthesize cDNA (TONBO Biosciences, 31-5300-0100R). qRT-PCR analysis was carried out with TONBO Biosciences CYBERFast™ qPCRHi-ROX Master Mix ...
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bioRxiv - Bioengineering 2023Quote: ... 1×106 PBMCs per well were stimulated for 48h in a CD3/CD28 (Tonbo Biosciences, 700037U100 and 70289U100 ...
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bioRxiv - Microbiology 2019Quote: ... Ghost Dye™ Violet 450 or Ghost Dye™ Violet 510 (Tonbo Biosciences, 1:200 dilution) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... live-dead stained with Ghost Dye Red 780 (1:1000 dilution, Tonbo Biosciences, San Diego CA), washed ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (Ly6eΔCD4: 1:800 anti-CD4-PECy5 [Tonbo Biosciences, CA]/1:400 anti-CD3ε-FITC [Tonbo Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded by a fixable viability dye (Ghost Red 710, Tonbo Biosciences, 1:6400). The following antibodies were used for flow cytometry:
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bioRxiv - Cell Biology 2024Quote: ... The cells were then stained using live/dead dye (1:1000, Ghost Dye Red 780, TONBO biosciences for myeloid ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent antibodies ...
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bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Immunology 2020Quote: ... The PBMCs were cultured in three different ways: 1) Plate-bound anti-CD3 + anti-CD28 (clones OKT3 and CD28.2 at 2μg/ml each - Tonbo Biosciences) - plates were coated with Goat anti-mouse IgG (clone Poly4053 at 10μg/ml - BioLegend ...
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bioRxiv - Immunology 2023Quote: Freshly thawed villous leukocytes (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... BM cell suspensions were lysed in 1 mL of red blood cell lysis buffer (Tonbo Biosciences, San Diego, CA), washed with 1X phosphate-buffered saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions were stained with 1:1000 Ghost dye violet 510 dead cell stain for 405nm excitation (Tonbo biosciences) in PBS for 30 min followed by a wash with 2% NCS HBSS ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BV570/BV605 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD3e (PE-Cy5/AF532 ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BUV395 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged and resuspended in FACS buffer (PBS supplemented with 2% FBS) and stained with Ghost Dye Violet 510 (1:1000, Tonbo Biosciences) on ice for 10 min for discrimination of viable and non-viable cells ...
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bioRxiv - Microbiology 2023Quote: ... 20 x 106 splenocytes were processed and stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Up to 20 x 106 lung cells were stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... About 500,000 cells were stained as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 510 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were washed thoroughly and stained for dead cell exclusion (Ghost Violet 510, 1:1000 dilution Tonbo Biosciences, San Diego, CA) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... CD45+ lymphocytes were flow sorted and 1 x 106 cells were used for staining with antibody cocktail (Supplementary Table 4) with anti-mouse CD16/32 (TONBO biosciences, 40-0161) for 30 minutes at room temperature in a final volume of 100uL in MaxPAR cell staining buffer (Fluidigm ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences; 13-0870). Surface antibodies ...
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bioRxiv - Immunology 2021Quote: ... 0.025% NaN3) and Fcγ receptors were blocked with 1/200 diluted anti-mouse CD16/32 antibodies (Fc block) (Tonbo Biosciences, San Diego, CA, USA) for 10 min on ice ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 naive DPB1*04:01 positive donors’ PBMCs were stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13- 0870-T100) and 1 µL of DPB1*04:01-S167-180-tetramer ...
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bioRxiv - Immunology 2021Quote: ... Cells were surface stained in an additional 50 uL FACS containing 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent anti-human antibodies ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Immunology 2023Quote: 96-well round bottom plates were coated with (0, 1, 10 μg/mL) anti-CD3ε (clone# 17A2, Ref# 40-0032, Tonbo Biosciences, San Diego, CA) or PBS for 2 hours at 37°C and removed by aspiration ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... 140 mM NaCl) and stained at room temperature with 1 µg/ml DAPI and 5 µl of Annexin V FITC (Tonbo Biosciences, San Diego, CA) for 15 minutes in the dark ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... slides were blocked and stained with a 1:250 dilution of rat anti-mouse CD45 primary antibody (Tonbo Biosciences; clone 30-F11, cat. #70-0451-U100), used in combination with a 1:500 secondary Alexa Fluor 647 donkey anti-rat antibody (Invitrogen Thermo Fisher Scientific ...