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Citations for Tonbo Biosciences :
1 - 50 of 76 citations for Glyphosate Chemical Purity 96% 2 13C 99%; 15N 98%+ 1000 Ug Ml In Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences, clone CD28.2; #70-0289-U100). For each electroporation 1 × 106 CD4+ T cells were pelleted by centrifugation at 100 x g for 10 mins and washed once in PBS ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged and resuspended in FACS buffer (PBS supplemented with 2% FBS) and stained with Ghost Dye Violet 510 (1:1000, Tonbo Biosciences) on ice for 10 min for discrimination of viable and non-viable cells ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... 20 x 106 splenocytes were processed and stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Up to 20 x 106 lung cells were stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3 coated plates [coated for 2 hours at 37°C with 20µg/mL anti-CD3 (UCHT1, Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2 ...
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bioRxiv - Microbiology 2024Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3–coated plates coated for 2 hours at 37°C with anti-CD3 (20 mg/ml) (UCHT1; Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
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bioRxiv - Immunology 2024Quote: ... PECy7 anti-mouse CD4 (clone GK1.5, Tonbo Biosciences, 1:1000), VioGreen anti-mouse CD8a (clone 53-6-7 ...
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bioRxiv - Immunology 2024Quote: ... PECy7 anti-mouse CD11c (clone N418, Tonbo Biosciences, 1:1000), PerPC/Cy5.5 anti-mouse CD24 (clone M1/69 ...
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bioRxiv - Cancer Biology 2022Quote: ... as well as GhostDye viability dye (Tonbo Biosciences #13-0865; 1:1000). The myeloid panel contained GhostDye (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... and IL-2-APC (Tonbo bioscience, 20-7021) on ice for 30 min ...
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bioRxiv - Genomics 2021Quote: ... live-dead stained with Ghost Dye Red 780 (1:1000 dilution, Tonbo Biosciences, San Diego CA), washed ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then stained using live/dead dye (1:1000, Ghost Dye Red 780, TONBO biosciences for myeloid ...
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bioRxiv - Bioengineering 2023Quote: ... 10ng/mL IL-15 (Tonbo Biosciences, 218157U002), and 10ng/mL IL-7 (Tonbo Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... The myeloid panel contained GhostDye (1:1000) and antibodies directed against CD45 (Tonbo Biosciences #50-0451; 1:250), CD11b (Tonbo Biosciences #75-0112 ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected from 1.0 × 106 lung cells/mL media restimulated overnight with plate-bound anti-CD3 (adsorbed to wells at 1.0 μg/ml in PBS) and soluble anti-CD28 (1.0 μg/ml) (Tonbo Biosciences), as previously described (36 ...
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bioRxiv - Immunology 2020Quote: ... and IL-4 (10 ng/mL; Tonbo Bioscience), or TLR ligand CpG (2.5 μM ...
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bioRxiv - Bioengineering 2023Quote: ... and 10ng/mL IL-7 (Tonbo Biosciences, 218079U002). Stimulated PBMCs were electroporated using the Neon transfection system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions were stained with 1:1000 Ghost dye violet 510 dead cell stain for 405nm excitation (Tonbo biosciences) in PBS for 30 min followed by a wash with 2% NCS HBSS ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BV570/BV605 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD3e (PE-Cy5/AF532 ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BUV395 ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL anti-CD28 (CD28.2, Tonbo Biosciences). CD4 T cells were purified as described above and 3 x 105 CD4 T cells were pelleted in a V-bottom plate and resuspended in 20 µL P3 buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.5μg/ml 7AAD (13-6993-T200, Tonbo Biosciences) in 2.5 mM Ca2+-containing RPMI for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience) plus 20 ng/mL recombinant human BAFF (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... About 500,000 cells were stained as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 510 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Immunology 2020Quote: ... and recombinant IL-4 cytokine (10 ng/mL; Tonbo Bioscience) with or without SCD inhibitor SSI-4 ...
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bioRxiv - Neuroscience 2021Quote: ... and brain-derived neurotrophic factor (BDNF; Tonbo Biosciences, 1μg/ml), with 50% media replacement every second day ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 μg/mL anti-mouse CD16/32 (TONBO Biosciences) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg/ml anti-mouse CD16/CD32 (2.4G2) (Tonbo Biosciences), surface antibodies and Fixable Viability Dye eFluor 780 (eBioscience) ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg/ml anti-mouse CD16/CD32 (2.4G2) (Tonbo Biosciences), surface antibodies and Fixable Viability Dye eFluor 780 (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were washed thoroughly and stained for dead cell exclusion (Ghost Violet 510, 1:1000 dilution Tonbo Biosciences, San Diego, CA) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... recombinant IL-4 and IL-5 (10 ng/mL; Tonbo Bioscience) cytokines ...
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bioRxiv - Immunology 2020Quote: Mice were intravenously (i.v.) injected with 2 µg of α-CD45 (Clone: 30-F11) (Tonbo Biosciences) diluted in 300 µL of sterile PBS five minutes before sacrifice ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then stained using live/dead dye (1:1000, Ghost Dye Red 780, TONBO biosciences for myeloid, 1:500, Ghost Dye Violet 450, TONBO biosciences for lymphoid) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2, Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C / 5% CO2 prior to electroporation.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were suspended in 0.5μg/ml 7-AAD (13-6993-T500, Tonbo Biosciences) in 1mL staining solution [900μL H2O + 100μL NaCitrate (380mM)] for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences). Cells were stimulated for 48 hours prior to electroporation.
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bioRxiv - Cancer Biology 2019Quote: ... or E4 (10ug/ml; Tonbo Biosciences 21-9195, 21-9189, and 21-9190) were added to the media ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences) at a final concentration of 2.5 × 106 cells/ml ...
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bioRxiv - Microbiology 2024Quote: ... Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml; CD28.2; Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C and 5% CO2 before treatment with KL-2.
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bioRxiv - Immunology 2023Quote: ... mice were intravenously injected by retro-orbital injection with 2 μg α-CD45-FITC (30-F11, Tonbo Biosciences) antibody to detect remaining circulating cells in lung samples (52) ...
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bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Microbiology 2020Quote: ... Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2, Tonbo Biosciences). Cells were stimulated for 72 h at 37°C in a 5%-CO2 atmosphere prior to electroporation ...
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bioRxiv - Microbiology 2023Quote: ... at a concentration of 10 µg/mL and anti-CD28 (Tonbo Biosciences #40-0289) supplemented to the media at a concentration of 5 µg/mL.