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Citations for Tonbo Biosciences :
101 - 144 of 144 citations for Anti NFIA Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... staining with APC Anti-Human CD3 (clone OKT3, 20-0037-T100, Tonbo Biosciences), monitoring GFP ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 PE-Cy7 (clone GL-1, Tonbo Biosciences, San Diego, CA), and anti-mouse F4/80 BUV395 (clone T45-2342 ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 1 μg Fc Block (anti-CD16/32, Tonbo Biosciences) for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences). Cells were stimulated for 48 hours prior to electroporation.
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bioRxiv - Immunology 2021Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences) at a final concentration of 2.5 × 106 cells/ml ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... prior to Fc-block incubation (anti-mouse CD16/CD32, clone 2.4G2, Tonbo Biosciences). CD8+ T cells were purified by negative selection using biotinylated antibodies and magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... incubated with anti-CD16/32 (clone 2.4G2, cat:70-0161-M001, TONBO biosciences) to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-CD3e-PerCP-Cyanine5.5 (145-2C11; 65-0031-U100) from Tonbo Biosciences. Streptavidin FITC (11-4317-87 ...
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bioRxiv - Microbiology 2024Quote: ... Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml; CD28.2; Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C and 5% CO2 before treatment with KL-2.
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bioRxiv - Immunology 2021Quote: ... stained with fluorochrome-conjugated antibodies and fixed with FOXP3/Transcription Factor Fixation Buffer (Tonbo Biosciences, Cat#: TNB-1020-L050). Tissue-derived cells were gated to exclude dead cells ...
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bioRxiv - Bioengineering 2020Quote: ... unstimulated and cytokine-stimulated 2D cultures of RAW264.7 macrophages were stained according to supplier instructions with both PE-conjugated CD86 antibody (Tonbo Biosciences) and FITC-conjugated CD206 antibody (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BV570/BV605 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD3e (PE-Cy5/AF532 ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BUV395 ...
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bioRxiv - Immunology 2021Quote: ... The APC-conjugated anti-FoxP3 mAb was purchased from Tonbo Biosciences (San Diego, CA). The FITC-conjugated mouse lineage marker cocktail (CD3 ...
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bioRxiv - Microbiology 2023Quote: ... at a concentration of 10 µg/mL and anti-CD28 (Tonbo Biosciences #40-0289) supplemented to the media at a concentration of 5 µg/mL.
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bioRxiv - Immunology 2024Quote: ... followed by intranuclear staining with an anti-Foxp3-APC Ab (clone; 3G3, TONBO Bioscience) using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT ...
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bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
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bioRxiv - Microbiology 2019Quote: ... and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences, clone CD28.2; #70-0289-U100). For each electroporation 1 × 106 CD4+ T cells were pelleted by centrifugation at 100 x g for 10 mins and washed once in PBS ...
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bioRxiv - Immunology 2019Quote: ... Non-specific binding was blocked using anti-mouse CD16/CD32 (BD Biosciences or Tonbo Biosciences). Viability staining solutions 7-amino-actinomycin D (7AAD ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with anti-mouse CD16/CD32 (clone: 2.4G2) (Tonbo Biosciences, San Diego, CA, USA) for 5 min on ice and washed ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences; 13-0870). Surface antibodies ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: PE anti-mouse FcεRI (clone MAR-1, Tonbo Biosciences, 1:500), FITC anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then incubated with anti-mouse CD45 (Tonbo Biosciences, San Diego, CA; Cat # 70-0451-U100) at a 1:200 dilution for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were collected after 45 min incubation at 37 °C and 5 % CO2 and stained with antibodies to microglia cell surface markers (CD11b-PE-Cy7, 1:200, Biolegend, #101216; CD45-APC-Cy7, 1:200, Tonbo Biosciences, #25-0459-T100). XO4+ and XO4− microglia uptake of pHrodo-conjugated synaptosomes or pHrodo™ Green E ...
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bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
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bioRxiv - Immunology 2022Quote: Following fluorescent dye- or biotin-conjugated antibodies (BioLegend, San Diego, CA, USA; Thermo Fisher Scientific, Waltham, MA, USA; BD Bioscience, San Jose, CA, USA; TONBO Biosciences, San Diego, CA, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected from 1.0 × 106 lung cells/mL media restimulated overnight with plate-bound anti-CD3 (adsorbed to wells at 1.0 μg/ml in PBS) and soluble anti-CD28 (1.0 μg/ml) (Tonbo Biosciences), as previously described (36 ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... after which 5×104 APCs and 1×105 T cells were co-cultured for 14-18 hours in the presence of 2μg/mL anti-CD28 (40-0281-M001, Tonbo Biosciences). T cell IFNγ production was detected via biotinylated IFNγ detection antibody (551881 ...
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bioRxiv - Immunology 2023Quote: ... were incubated overnight at 4°C with PBS containing plate-bound anti-CD3χ (4 μg/ml, 145-2C11, Tonbo Biosciences) and anti-CD28 (4 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... no. 40-0032-U500) and anti-mouse CD28 (Cat. no. 40-0281-U500) [functional assay grade] were procured from Tonbo Biosciences, San Diego ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were stimulated with 1 nM SIINFEKL (OT-I CD8+ T cells) or in wells coated with anti-CD3 (10 μg/ml; clone: 145-2C11; Tonbo Biosciences) and anti-CD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: BMDCs and MLN cells were stained with the following Abs to identify DCs: anti-CD11c-PE/Cyanine 7 (clone; N418, TONBO Biosciences) and anti-I-A/I-E-PerCP (clone ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (Tonbo Biosciences Cat# 70-0031 (also OWL-A11492), RRID:AB_2621472 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Immunology 2023Quote: 96-well round bottom plates were coated with (0, 1, 10 μg/mL) anti-CD3ε (clone# 17A2, Ref# 40-0032, Tonbo Biosciences, San Diego, CA) or PBS for 2 hours at 37°C and removed by aspiration ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer’s protocol then plated in 96-well round bottom plates and cultured in the presence of anti-CD28 (0, 4, 10 μg/mL, Ref# 40-0281, Tonbo Biosciences, San Diego, CA) and/ or capsaicin ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...