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Citations for Tonbo Biosciences :
151 - 200 of 201 citations for Mouse Anti Toxoplasma gondii SAG 1 protein 6207 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... as indicated: Ghost viability dye (1:400; Tonbo Biosciences Cat. 13-0870-T100); CD4-AF488 (1:300 ...
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bioRxiv - Immunology 2021Quote: ... CD3e (PE-Cy5/AF532, Tonbo Bioscience/Thermo Fischer, 1:200, clone 145-2C11), CD4 (PerCP/BV605 ...
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bioRxiv - Immunology 2023Quote: ... CD3e (PE-Cy5/AF532, Tonbo Bioscience/Thermo Fischer, 1:200, clone 145-2C11), CD4 (PerCP/BV605 ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected from 1.0 × 106 lung cells/mL media restimulated overnight with plate-bound anti-CD3 (adsorbed to wells at 1.0 μg/ml in PBS) and soluble anti-CD28 (1.0 μg/ml) (Tonbo Biosciences), as previously described (36 ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in Fc block (1:100, Tonbo Bioscience, 70-0161-U500) followed by indicated antibodies and fixed with 1% PFA in flow cytometry buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 µg total RNA was used to cDNA synthesis (TONBO Biosciences, 31-5300-0100R). qRT-PCR studies were performed using TONBO Biosciences CYBER Fast™ qPCR Hi-ROX Master Mix ...
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bioRxiv - Immunology 2021Quote: ... PD-L1 (PE/BV711/PE-Cy7, Tonbo Biosciences/Biolegend, 1:100, clone 10F.9G2), CD44 (AF700 ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... after which 5×104 APCs and 1×105 T cells were co-cultured for 14-18 hours in the presence of 2μg/mL anti-CD28 (40-0281-M001, Tonbo Biosciences). T cell IFNγ production was detected via biotinylated IFNγ detection antibody (551881 ...
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bioRxiv - Immunology 2023Quote: ... were incubated overnight at 4°C with PBS containing plate-bound anti-CD3χ (4 μg/ml, 145-2C11, Tonbo Biosciences) and anti-CD28 (4 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Intranuclear staining for Tcf-1 was performed using a Foxp3 staining buffer set (Tonbo bioscience) and stained with anti-Tcf-1 (C63D9 ...
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bioRxiv - Microbiology 2020Quote: HeLa cells were pretreated with 500 U ml−1 IFN-β (Tonbo Biosciences 21-8699) or vehicle (0.1% BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells from BALF were stained with CD16/32 (Tonbo biosciences 70-0161-u500, 1:10) to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was used to synthesize cDNA (TONBO Biosciences, 31-5300-0100R). qRT-PCR analysis was carried out with TONBO Biosciences CYBERFast™ qPCRHi-ROX Master Mix ...
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bioRxiv - Bioengineering 2023Quote: ... 1×106 PBMCs per well were stimulated for 48h in a CD3/CD28 (Tonbo Biosciences, 700037U100 and 70289U100 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for 20 minutes at RT with mouse PE conjugated 5-OP-RU MR1-tetramer (NIH Tetramer core) and the following antibodies: anti-CD4-FITC/APC-Fire810 (clone GK1.5, Tonbo Biosciences/Biolegend), anti-CD8α-AF700 (clone 53-6.7 ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were stimulated with 1 nM SIINFEKL (OT-I CD8+ T cells) or in wells coated with anti-CD3 (10 μg/ml; clone: 145-2C11; Tonbo Biosciences) and anti-CD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: BMDCs and MLN cells were stained with the following Abs to identify DCs: anti-CD11c-PE/Cyanine 7 (clone; N418, TONBO Biosciences) and anti-I-A/I-E-PerCP (clone ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (Tonbo Biosciences Cat# 70-0031 (also OWL-A11492), RRID:AB_2621472 ...
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bioRxiv - Microbiology 2019Quote: ... Ghost Dye™ Violet 450 or Ghost Dye™ Violet 510 (Tonbo Biosciences, 1:200 dilution) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... live-dead stained with Ghost Dye Red 780 (1:1000 dilution, Tonbo Biosciences, San Diego CA), washed ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded by a fixable viability dye (Ghost Red 710, Tonbo Biosciences, 1:6400). The following antibodies were used for flow cytometry:
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bioRxiv - Immunology 2021Quote: ... The following stains and antibody-fluorophore combinations were used to assess cellular phenotype: anti-B220-PE-Cy7 or -AlexaFluor700 (Tonbo Biosciences, RA3-6B2) at 0.5 μl per 1 x 106 cells ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then stained using live/dead dye (1:1000, Ghost Dye Red 780, TONBO biosciences for myeloid ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer’s protocol then plated in 96-well round bottom plates and cultured in the presence of anti-CD28 (0, 4, 10 μg/mL, Ref# 40-0281, Tonbo Biosciences, San Diego, CA) and/ or capsaicin ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent antibodies ...
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bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Immunology 2023Quote: Freshly thawed villous leukocytes (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... BM cell suspensions were lysed in 1 mL of red blood cell lysis buffer (Tonbo Biosciences, San Diego, CA), washed with 1X phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions were stained with 1:1000 Ghost dye violet 510 dead cell stain for 405nm excitation (Tonbo biosciences) in PBS for 30 min followed by a wash with 2% NCS HBSS ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BV570/BV605 ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BUV395 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD3e (PE-Cy5/AF532 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged and resuspended in FACS buffer (PBS supplemented with 2% FBS) and stained with Ghost Dye Violet 510 (1:1000, Tonbo Biosciences) on ice for 10 min for discrimination of viable and non-viable cells ...
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bioRxiv - Microbiology 2023Quote: ... 20 x 106 splenocytes were processed and stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Up to 20 x 106 lung cells were stained in 15 mL conical tubes as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 450 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... About 500,000 cells were stained as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 510 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% penicillin-streptomycin-fungizone [PSF]) or basal media supplemented with 10 ng/mL of IL-1β (human recombinant; Tonbo Bioscience 218018U010) for 2 weeks ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were washed thoroughly and stained for dead cell exclusion (Ghost Violet 510, 1:1000 dilution Tonbo Biosciences, San Diego, CA) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences; 13-0870). Surface antibodies ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 naive DPB1*04:01 positive donors’ PBMCs were stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13- 0870-T100) and 1 µL of DPB1*04:01-S167-180-tetramer ...
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bioRxiv - Immunology 2021Quote: ... Cells were surface stained in an additional 50 uL FACS containing 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent anti-human antibodies ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... 140 mM NaCl) and stained at room temperature with 1 µg/ml DAPI and 5 µl of Annexin V FITC (Tonbo Biosciences, San Diego, CA) for 15 minutes in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...