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Citations for Tonbo Biosciences :
101 - 144 of 144 citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 DbpA Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... staining with APC Anti-Human CD3 (clone OKT3, 20-0037-T100, Tonbo Biosciences), monitoring GFP ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 PE-Cy7 (clone GL-1, Tonbo Biosciences, San Diego, CA), and anti-mouse F4/80 BUV395 (clone T45-2342 ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 1 μg Fc Block (anti-CD16/32, Tonbo Biosciences) for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences). Cells were stimulated for 48 hours prior to electroporation.
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bioRxiv - Immunology 2021Quote: ... in the presence of 5 μg/ml soluble anti-CD28 (CD28.2, Tonbo Biosciences) at a final concentration of 2.5 × 106 cells/ml ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... prior to Fc-block incubation (anti-mouse CD16/CD32, clone 2.4G2, Tonbo Biosciences). CD8+ T cells were purified by negative selection using biotinylated antibodies and magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... incubated with anti-CD16/32 (clone 2.4G2, cat:70-0161-M001, TONBO biosciences) to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-CD3e-PerCP-Cyanine5.5 (145-2C11; 65-0031-U100) from Tonbo Biosciences. Streptavidin FITC (11-4317-87 ...
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bioRxiv - Microbiology 2024Quote: ... Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml; CD28.2; Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C and 5% CO2 before treatment with KL-2.
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bioRxiv - Immunology 2021Quote: ... stained with fluorochrome-conjugated antibodies and fixed with FOXP3/Transcription Factor Fixation Buffer (Tonbo Biosciences, Cat#: TNB-1020-L050). Tissue-derived cells were gated to exclude dead cells ...
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bioRxiv - Bioengineering 2020Quote: ... unstimulated and cytokine-stimulated 2D cultures of RAW264.7 macrophages were stained according to supplier instructions with both PE-conjugated CD86 antibody (Tonbo Biosciences) and FITC-conjugated CD206 antibody (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BV570/BV605 ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BUV395 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD3e (PE-Cy5/AF532 ...
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bioRxiv - Immunology 2021Quote: ... The APC-conjugated anti-FoxP3 mAb was purchased from Tonbo Biosciences (San Diego, CA). The FITC-conjugated mouse lineage marker cocktail (CD3 ...
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bioRxiv - Microbiology 2023Quote: ... at a concentration of 10 µg/mL and anti-CD28 (Tonbo Biosciences #40-0289) supplemented to the media at a concentration of 5 µg/mL.
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bioRxiv - Immunology 2024Quote: ... followed by intranuclear staining with an anti-Foxp3-APC Ab (clone; 3G3, TONBO Bioscience) using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT ...
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bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
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bioRxiv - Microbiology 2019Quote: ... and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences, clone CD28.2; #70-0289-U100). For each electroporation 1 × 106 CD4+ T cells were pelleted by centrifugation at 100 x g for 10 mins and washed once in PBS ...
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bioRxiv - Immunology 2019Quote: ... Non-specific binding was blocked using anti-mouse CD16/CD32 (BD Biosciences or Tonbo Biosciences). Viability staining solutions 7-amino-actinomycin D (7AAD ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with anti-mouse CD16/CD32 (clone: 2.4G2) (Tonbo Biosciences, San Diego, CA, USA) for 5 min on ice and washed ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences; 13-0870). Surface antibodies ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: PE anti-mouse FcεRI (clone MAR-1, Tonbo Biosciences, 1:500), FITC anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then incubated with anti-mouse CD45 (Tonbo Biosciences, San Diego, CA; Cat # 70-0451-U100) at a 1:200 dilution for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were collected after 45 min incubation at 37 °C and 5 % CO2 and stained with antibodies to microglia cell surface markers (CD11b-PE-Cy7, 1:200, Biolegend, #101216; CD45-APC-Cy7, 1:200, Tonbo Biosciences, #25-0459-T100). XO4+ and XO4− microglia uptake of pHrodo-conjugated synaptosomes or pHrodo™ Green E ...
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bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
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bioRxiv - Immunology 2022Quote: Following fluorescent dye- or biotin-conjugated antibodies (BioLegend, San Diego, CA, USA; Thermo Fisher Scientific, Waltham, MA, USA; BD Bioscience, San Jose, CA, USA; TONBO Biosciences, San Diego, CA, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected from 1.0 × 106 lung cells/mL media restimulated overnight with plate-bound anti-CD3 (adsorbed to wells at 1.0 μg/ml in PBS) and soluble anti-CD28 (1.0 μg/ml) (Tonbo Biosciences), as previously described (36 ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... after which 5×104 APCs and 1×105 T cells were co-cultured for 14-18 hours in the presence of 2μg/mL anti-CD28 (40-0281-M001, Tonbo Biosciences). T cell IFNγ production was detected via biotinylated IFNγ detection antibody (551881 ...
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bioRxiv - Immunology 2023Quote: ... were incubated overnight at 4°C with PBS containing plate-bound anti-CD3χ (4 μg/ml, 145-2C11, Tonbo Biosciences) and anti-CD28 (4 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... no. 40-0032-U500) and anti-mouse CD28 (Cat. no. 40-0281-U500) [functional assay grade] were procured from Tonbo Biosciences, San Diego ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were stimulated with 1 nM SIINFEKL (OT-I CD8+ T cells) or in wells coated with anti-CD3 (10 μg/ml; clone: 145-2C11; Tonbo Biosciences) and anti-CD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: BMDCs and MLN cells were stained with the following Abs to identify DCs: anti-CD11c-PE/Cyanine 7 (clone; N418, TONBO Biosciences) and anti-I-A/I-E-PerCP (clone ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (Tonbo Biosciences Cat# 70-0031 (also OWL-A11492), RRID:AB_2621472 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Immunology 2023Quote: 96-well round bottom plates were coated with (0, 1, 10 μg/mL) anti-CD3ε (clone# 17A2, Ref# 40-0032, Tonbo Biosciences, San Diego, CA) or PBS for 2 hours at 37°C and removed by aspiration ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer’s protocol then plated in 96-well round bottom plates and cultured in the presence of anti-CD28 (0, 4, 10 μg/mL, Ref# 40-0281, Tonbo Biosciences, San Diego, CA) and/ or capsaicin ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...