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Citations for Tonbo Biosciences :
101 - 139 of 139 citations for Anti CD117 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with anti-mouse CD16/CD32 (clone: 2.4G2) (Tonbo Biosciences, San Diego, CA, USA) for 5 min on ice and washed ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (Ly6eΔCD4: 1:800 anti-CD4-PECy5 [Tonbo Biosciences, CA]/1:400 anti-CD3ε-FITC [Tonbo Biosciences ...
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bioRxiv - Immunology 2021Quote: ... or fixed overnight at 4°C for intracellular staining the next day using a FOXP3 kit (Tonbo Biosciences). The staining was performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Permeabilization and intracellular staining steps were performed with the FoxP3 permeabilization buffer kit (Tonbo Biosciences, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse CD8α-APC antibody (0.7 μL; cat. #20-1886, Clone 2.43, Tonbo Biosciences, San Diego, CA) was added then incubated for 10 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: PE anti-mouse FcεRI (clone MAR-1, Tonbo Biosciences, 1:500), FITC anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then incubated with anti-mouse CD45 (Tonbo Biosciences, San Diego, CA; Cat # 70-0451-U100) at a 1:200 dilution for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Samples were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Kit (Tonbo Biosciences, San Diego, CA, U.S.), then incubated with primary-conjugated antibodies for 45 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with the Foxp3 Transcription factor staining Buffer kit according to manufacturer’s recommendations (Tonbo Biosciences), followed by intracellular staining with anti-Foxp3-PE (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in the study are as follows: APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences), Alexa Fluor 700 anti-mouse CD45 (Clone ...
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bioRxiv - Immunology 2023Quote: ... Additional antibodies used for this experiment included anti-IFNψ-APC (Tonbo Biosciences Cat# 20-7311 (also OWL-A01038), RRID:AB_2621616) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and resuspended in Transcription Factor Fix/Perm solution prepared as per manufacturer’s protocol (TNB-0607-KIT; Tonbo Biosciences). Following 30 mins incubation at RT ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with 1x Flow Cytometry Perm Buffer prepared as per manufacturer’s instructions (TNB-0607-KIT; Tonbo Biosciences) before staining with intracellular marker antibodies for 30 mins at 4°C in the dark (Table S2).
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected from 1.0 × 106 lung cells/mL media restimulated overnight with plate-bound anti-CD3 (adsorbed to wells at 1.0 μg/ml in PBS) and soluble anti-CD28 (1.0 μg/ml) (Tonbo Biosciences), as previously described (36 ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse CD16/CD32 Fc Block antibody purchased at a concentration of 2mg/mL (Tonbo Biosciences 40-0161-M001) was added at 0.5 uL per sample (in 100uL total volume per sample of block antibody diluted in PBS ...
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bioRxiv - Microbiology 2020Quote: ... the ELISA kits to determine the levels of IFNγ and TNFα from house mouse (Mus muscuslus) (Tonbo Bioscience, San Diego, CA) were utilized to detect those cytokines in white-footed mice ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... after which 5×104 APCs and 1×105 T cells were co-cultured for 14-18 hours in the presence of 2μg/mL anti-CD28 (40-0281-M001, Tonbo Biosciences). T cell IFNγ production was detected via biotinylated IFNγ detection antibody (551881 ...
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bioRxiv - Immunology 2023Quote: ... were incubated overnight at 4°C with PBS containing plate-bound anti-CD3χ (4 μg/ml, 145-2C11, Tonbo Biosciences) and anti-CD28 (4 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... no. 40-0032-U500) and anti-mouse CD28 (Cat. no. 40-0281-U500) [functional assay grade] were procured from Tonbo Biosciences, San Diego ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for 20 minutes at RT with mouse PE conjugated 5-OP-RU MR1-tetramer (NIH Tetramer core) and the following antibodies: anti-CD4-FITC/APC-Fire810 (clone GK1.5, Tonbo Biosciences/Biolegend), anti-CD8α-AF700 (clone 53-6.7 ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were stimulated with 1 nM SIINFEKL (OT-I CD8+ T cells) or in wells coated with anti-CD3 (10 μg/ml; clone: 145-2C11; Tonbo Biosciences) and anti-CD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: BMDCs and MLN cells were stained with the following Abs to identify DCs: anti-CD11c-PE/Cyanine 7 (clone; N418, TONBO Biosciences) and anti-I-A/I-E-PerCP (clone ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (Tonbo Biosciences Cat# 70-0031 (also OWL-A11492), RRID:AB_2621472 ...
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bioRxiv - Immunology 2021Quote: ... The following stains and antibody-fluorophore combinations were used to assess cellular phenotype: anti-B220-PE-Cy7 or -AlexaFluor700 (Tonbo Biosciences, RA3-6B2) at 0.5 μl per 1 x 106 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... CD45+ lymphocytes were flow sorted and 1 x 106 cells were used for staining with antibody cocktail (Supplementary Table 4) with anti-mouse CD16/32 (TONBO biosciences, 40-0161) for 30 minutes at room temperature in a final volume of 100uL in MaxPAR cell staining buffer (Fluidigm ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Immunology 2021Quote: ... 0.025% NaN3) and Fcγ receptors were blocked with 1/200 diluted anti-mouse CD16/32 antibodies (Fc block) (Tonbo Biosciences, San Diego, CA, USA) for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Immunology 2023Quote: 96-well round bottom plates were coated with (0, 1, 10 μg/mL) anti-CD3ε (clone# 17A2, Ref# 40-0032, Tonbo Biosciences, San Diego, CA) or PBS for 2 hours at 37°C and removed by aspiration ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer’s protocol then plated in 96-well round bottom plates and cultured in the presence of anti-CD28 (0, 4, 10 μg/mL, Ref# 40-0281, Tonbo Biosciences, San Diego, CA) and/ or capsaicin ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... slides were blocked and stained with a 1:250 dilution of rat anti-mouse CD45 primary antibody (Tonbo Biosciences; clone 30-F11, cat. #70-0451-U100), used in combination with a 1:500 secondary Alexa Fluor 647 donkey anti-rat antibody (Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Cells were incubated with anti-mouse primary antibodies to assess immune populations with the following conjugated antibodies: B220-APC (Tonbo Biosciences, San Diego, CA; clone RA3-6B2), CD19-PE/Dazzle594 (BioLegend ...