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• GM-CSF-mediated epithelial-immune cell crosstalk orchestrates pulmonary immunity to Aspergillus fumigatus

  Kathleen A. M. Mills, et al., bioRxiv - Immunology 2024

  Quote: ... red blood cells were lysed using RBC lysis buffer (Tonbo Biosciences), cells were blocked with anti-CD16/CD32 ...
• The ESCRT protein CHMP5 promotes T cell leukemia by controlling BRD4-p300-dependent transcription

  Katharine Umphred-Wilson, et al., bioRxiv - Cancer Biology 2024

  Quote: ... incubated with 7-AAD (Tonbo Bioscience #13-6993-T500) for 20 minutes at RT ...
• Modeling platinum resistance in a stem-like patient-derived ovarian cancer sample

  Tise Suzuki, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and 7AAD (13-6993-T500; Tonbo Biosciences, San Diego, CA, USA). One hundred thousand cells were plated per well in a 12-well plate ...
• Using human genetics to develop strategies to increase erythropoietic output from genome-edited hematopoietic stem and progenitor cells

  Sofia E. Luna, et al., bioRxiv - Genetics 2024

  Quote: ... cells were also stained with Ghost Dye Red 780 (Tonbo Biosciences, San Diego, CA, USA) to measure viability.
• Brainstem Dbh⁺ Neurons Control Chronic Allergen-Induced Airway Hyperreactivity

  Yujuan Su, et al., bioRxiv - Cell Biology 2024

  Quote: ... The cells were then stained using live/dead dye (1:1000, Ghost Dye Red 780, TONBO biosciences for myeloid ...
• In Situ-Crosslinked Zippersomes Enhance Cardiac Repair by Increasing Accumulation and Retention

  Natalie E. Jasiewicz, et al., bioRxiv - Bioengineering 2024

  Quote: ... This was followed by an HRP secondary antibody (Tonbo Biosciences, San Diego, CA, Cat # 72-8104-M001). Chromogen was developed with a DAB HRP substrate (Vector Labs ...
• In Situ-Crosslinked Zippersomes Enhance Cardiac Repair by Increasing Accumulation and Retention

  Natalie E. Jasiewicz, et al., bioRxiv - Bioengineering 2024

  Quote: ... Sections were then incubated with anti-mouse CD45 (Tonbo Biosciences, San Diego, CA; Cat # 70-0451-U100) at a 1:200 dilution for 30 minutes at room temperature ...
• Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence

  Dan Cappabianca, et al., bioRxiv - Bioengineering 2024

  Quote: ... Cells were then stained for viability with either GhostRed 780 (Catalog # 50-105-2988, Tonbo Biosciences, San Diego, CA) or Live-Dead Blue (Catalog # L23105 ...
• Regulation of fatty acid delivery to metastases by tumor endothelium

  Deanna N. Edwards, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Ghost Dye Violet 510 (Tonbo Biosciences #13-0870). Blocking with anti-CD16/32 (Tonbo #70-0161 ...
• An AhR-Ovol1-Id1 regulatory axis in keratinocytes promotes skin homeostasis against atopic dermatitis

  Zeyu Chen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... APC/Cyanine7 anti-mouse Ly6g (Tonbo Biosciences, Cat# 25-1276, Clone 1A8), APC anti-mouse CD11c (Biolegend ...
• CD4+ and CD8+ T cells are required to prevent SARS-CoV-2 persistence in the nasal compartment

  Meenakshi Kar, et al., bioRxiv - Immunology 2024

  Quote: ... the cell suspensions were stained using surface stain antibodies including Live/Dead Ghost Dye stain (Tonbo Biosciences) for 20 minutes at 4°C ...
• Heterologous Prime-Boost with Immunologically Orthogonal Protein Nanoparticles for Peptide Immunofocusing

  Sonia Bhattacharya, et al., bioRxiv - Immunology 2024

  Quote: ... Anti-mouse CD16/CD32 Fc Block antibody purchased at a concentration of 2mg/mL (Tonbo Biosciences 40-0161-M001) was added at 0.5 uL per sample (in 100uL total volume per sample of block antibody diluted in PBS ...
• Polymeric nanoparticles delivery of AMPK activator 991 prevents its toxicity and improves muscle homeostasis in Duchenne Muscular Dystrophy

  Ilaria Andreana, et al., bioRxiv - Pathology 2024

  Quote: ... dead cells were first labeled with Ghost Dye Red 780 (13-0865-T500, Tonbo Biosciences) for 30 min at 4°C ...
• Macrophages protect against sensory axon degeneration in diabetic neuropathy

  Sara Hakim, et al., bioRxiv - Neuroscience 2024

  Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...
• Macrophages protect against sensory axon degeneration in diabetic neuropathy

  Sara Hakim, et al., bioRxiv - Neuroscience 2024

  Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
• Roles of C/EBPα, GATA2, TGF-β-signaling, and epigenetic regulation in the expression of basophil-specific protease genes

  Ryotaro Tojima, et al., bioRxiv - Immunology 2024

  Quote: ... Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences), and recombinant mouse TGF-β (5231 ...
• Roles of C/EBPα, GATA2, TGF-β-signaling, and epigenetic regulation in the expression of basophil-specific protease genes

  Ryotaro Tojima, et al., bioRxiv - Immunology 2024

  Quote: ... which were obtained from indicated sources: PE anti-mouse FcεRI (clone MAR-1, Tonbo Biosciences, 1:500), FITC anti-mouse CD117 (clone 2B8 ...
• Combined Dendritic Cell And Anti-TIGIT Immunotherapy Potentiate Trail+ Memory NK Cells Against HIV-1 Infected Cells

  I Sánchez-Cerrillo, et al., bioRxiv - Immunology 2024

  Quote: The analysis of NK cell subpopulations was performed using a Ghost Dye Red 780 viability dye (TONBO Biosciences), anti-CD56 ...
• DMSO derives Trophectoderm and Clonal Blastoid from Single Human Pluripotent Stem Cell

  Samhan Alsolami, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Each sample was stained with 10 μL propidium iodide (PI) staining solution (Tonbo Biosciences, cat# 13-6990-T200) for 20 min ...
• PP2A catalytic subunit alpha is critically required for CD8⁺ T cell homeostasis and anti-bacterial responses

  Xian Zhou, et al., bioRxiv - Immunology 2024

  Quote: ... Cell viability was assessed using the Fixable Dye Ghost 540 (Tonbo Bioscience), or 7-AAD ...
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... anti-CD8-PE/Cy7 (#60–0081, TONBO Biosciences, San Diego, CA), anti-CD25-PerCP (#65-0251 ...
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... anti-CD19-APC (#20-0193, TONBO Biosciences), anti-Ki67-FITC (#11-5698-82 ...
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... anti-CD25-PerCP (#65-0251, TONBO Biosciences), anti-Foxp3-PE (#12-5773-82 ...
• Metabolic Deficiencies Underlie Plasmacytoid Dendritic Cell Exhaustion After Viral Infection

  Trever T. Greene, et al., bioRxiv - Immunology 2024

  Quote: ... or Ghost dye (Tonbo Biosciences, San Diego, CA) was used to exclude dead cells ...
• Kaempferol exerts anti-inflammatory effects by accelerating Treg development via AhR-mediated and PU.1/IRF4-dependent transactivation of the Aldh1a2/RALDH2 gene in dendritic cells

  Miki Takahashi, et al., bioRxiv - Immunology 2024

  Quote: BMDCs and MLN cells were stained with the following Abs to identify DCs: anti-CD11c-PE/Cyanine 7 (clone; N418, TONBO Biosciences) and anti-I-A/I-E-PerCP (clone ...
• Kaempferol exerts anti-inflammatory effects by accelerating Treg development via AhR-mediated and PU.1/IRF4-dependent transactivation of the Aldh1a2/RALDH2 gene in dendritic cells

  Miki Takahashi, et al., bioRxiv - Immunology 2024

  Quote: ... followed by intranuclear staining with an anti-Foxp3-APC Ab (clone; 3G3, TONBO Bioscience) using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT ...
• Kaempferol exerts anti-inflammatory effects by accelerating Treg development via AhR-mediated and PU.1/IRF4-dependent transactivation of the Aldh1a2/RALDH2 gene in dendritic cells

  Miki Takahashi, et al., bioRxiv - Immunology 2024

  Quote: ... using the Foxp3/Transcription Factor Staining Buffer Kit (#TNB-0607-KIT, TONBO Biosciences) was performed ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... PECy7 anti-mouse CD4 (clone GK1.5, Tonbo Biosciences, 1:1000), VioGreen anti-mouse CD8a (clone 53-6-7 ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... PE anti-mouse CD62L (clone MEL-14, Tonbo Biosciences, 1:100), FITC anti-mouse CD86 (clone GL-1 ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... APCCy7 anti-human/mouse CD11b (clone M1/70, Tonbo Biosciences, 1:2000), PECy7 anti-mouse CD11c (clone N418 ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: DAPI (#11034-56, Nacalai Tescue) and Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences) were added to the cells just before staining with specific antibodies against cell surface molecules ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... PECy7 anti-mouse CD11c (clone N418, Tonbo Biosciences, 1:1000), PerPC/Cy5.5 anti-mouse CD24 (clone M1/69 ...
• The nutrient-sensing Rag-GTPase complex in B cells controls humoral immunity via TFEB/TFE3-dependent mitochondrial fitness

  Xingxing Zhu, et al., bioRxiv - Immunology 2024

  Quote: ... Cell viability was examined by Fixable viability dye (Tonbo Bioscience) or 7-AAD (Thermo Fisher Scientific ...
• The nutrient-sensing Rag-GTPase complex in B cells controls humoral immunity via TFEB/TFE3-dependent mitochondrial fitness

  Xingxing Zhu, et al., bioRxiv - Immunology 2024

  Quote: ... 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience) plus 20 ng/mL recombinant human BAFF (Biolegend ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: ... Ly6G-PE (Tonbo Biosciences, clone RB6-8C5), and F4/80-FITC (Tonbo Biosciences ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: ... and F4/80-FITC (Tonbo Biosciences, clone BM8.1,). Relevant isotype controls were used for each experiment ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: ... CD11c-PerCp/Cy5.5 (Tonbo Biosciences, clone N418), Ly6G-PE (Tonbo Biosciences ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: ... CD8-PE/Cy7 (Tonbo Biosciences, clone 53-6.7), NK1.1-PE (Tonbo Biosciences ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: ... CD11b-APC/Cy7 (Tonbo Biosciences, clone M1/70), CD11c-PerCp/Cy5.5 (Tonbo Biosciences ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: ... CD4-violetFluor450 (Tonbo Biosciences, clone GK1.5), CD8-PE/Cy7 (Tonbo Biosciences ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: Cells were incubated with anti-mouse primary antibodies to assess immune populations with the following conjugated antibodies: B220-APC (Tonbo Biosciences, San Diego, CA; clone RA3-6B2), CD19-PE/Dazzle594 (BioLegend ...
• Assessing a Role for Sarcospan in Immune Function

  Isela C. Valera, et al., bioRxiv - Immunology 2024

  Quote: ... NK1.1-PE (Tonbo Biosciences, clone PK136), CD335/NKp46-APC (BioLegend ...
• A regulatory B cell subpopulation expressing CD301b lectin promotes breast cancer growth

  Amy V. Paschall, Zahra Nawaz, Fikri Y Avci, bioRxiv - Immunology 2024

  Quote: ... and Ghost Red 710 (Tonbo Biosciences). For intracellular staining ...
• Targeting PD-L1 in solid cancer with myeloid cells expressing a CAR-like immune receptor

  Kayla Myers Chen, et al., bioRxiv - Immunology 2024

  Quote: ... APC Streptavidin (Tonbo Biosciences); Zombie NIR viability dye from BioLegend ...
• Targeting PD-L1 in solid cancer with myeloid cells expressing a CAR-like immune receptor

  Kayla Myers Chen, et al., bioRxiv - Immunology 2024

  Quote: ... APC mouse PD-L1 from Tonbo Biosciences. In addition ...
• First-in-class Transactivator-Free, Doxycycline-inducible IL-18-engineered CAR-T cells for relapsed/refractory B-cell lymphomas

  Pedro Justicia-Lirio, et al., bioRxiv - Immunology 2024

  Quote: ... cell viability was determined using Ghost Dye Violet 510 (TONBO biosciences). Absolute cell quantification was performed using CountBright Absolute Counting Beads (ThermoFisher) ...
• Cytotoxic CD4⁺ T cells in the bone marrow compromise healthy ageing by enhancing granulopoiesis

  Enrique Gabandé-Rodríguez, et al., bioRxiv - Immunology 2024

  Quote: ... or the Ghost Dye™ Violet 540 (Tonbo Biosciences, 13-0879) for 20 min at 4°C ...
• Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal

  William J. Cisneros, et al., bioRxiv - Microbiology 2024

  Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3–coated plates coated for 2 hours at 37°C with anti-CD3 (20 mg/ml) (UCHT1; Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml ...
• Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal

  William J. Cisneros, et al., bioRxiv - Microbiology 2024

  Quote: ... Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml; CD28.2; Tonbo Biosciences). Cells were stimulated for 72 hours at 37°C and 5% CO2 before treatment with KL-2.
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... and viability dye Ghost Dye™ Violet 540 (Tonbo Bioscience, San Diego, CA, USA, cat. 130879T100). Samples were analyzed on a CytoFLEX flow cytometer (Beckman-Coulter ...