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Citations for Applied Biological Materials :
51 - 100 of 143 citations for hsa mir 146a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The viral titer was determined by RT‒qPCR using the titer kit from Applied Biological Materials prior to use.
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... and the HRP signal was visualized with ECL Western Blotting Detection reagents (ABM) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was performed using the ABM all-in-one 5X RT Mastermix kit (ABM, Richmond, Canada). Primer Express 3.0 software was used for designing primers and quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... PCR were run using a Taq DNA polymerases Kit (ABM, Richmond, B.C) and a thermal cycler (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA was synthesized from the isolated RNA by reverse transcription using RevertAid™ first strand cDNA synthesis kit (5× All-In-One RT MasterMix, ABM) according to the manufacturer’s instructions in a RNase-free environment ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from erythroid/megakaryocytic progenitor or K562 cells using the RNA Isolation Kit (Omn-02, Omiget, China) and reverse-transcribed into cDNA with the All-In-One 5X RT MasterMix (G592, ABM, Canada). For qRT-PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... The mRNA and miRNA reverse transcription were performed within a 5×All-In-One RT MasterMix kit (Applied Biological Materials, BC, Canada) and TaqMan microRNA Reverse Transcription Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: cDNA synthesis was carried out with isolated total RNA using 5 X All-In-One RT MasterMix kit (Applied Biological Materials, G490) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... cDNA was obtained using RT MasterMix (Applied Biological Materials) and used to perform a SYBR-based real-time PCR (BioLine ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was then generated using the 5X RT Mastermix (ABM, Vancouver, Canada). Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using BrightGreen 2X qPCR MasterMix (ABM ...
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bioRxiv - Physiology 2023Quote: ... RNA samples underwent reverse transcription (All-in-one RT Master Mix – ABM). qPCR was performed with BrightGreen Express reagent (ABM ...
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bioRxiv - Microbiology 2019Quote: ... Total cDNA was synthesized using the All-In-One RT Master Mix (ABM). For RT-qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription was performed using All-in-One 5X RT MasterMix (ABM, G592). Primer Express 3.0 software was used to design primers and qRT-PCR was performed as previously described (83,84 ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was then synthesized using All-In-One RT MasterMix (Applied Biological Materials). qPCR was carried out using TB Green Premix Ex Taq II (Takara Bio ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcription was carried out using ‘5× All-in-one RT Mastermix’ (#g486; ABM), following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: All 2D and 3D cultures were assessed for mycoplasma monthly via the highly sensitive PCR-based kit from ABM (cat. G238). Where applicable ...
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bioRxiv - Microbiology 2019Quote: ... staining or PCR (G238, ABM).
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bioRxiv - Genomics 2020Quote: ... the First-strand cDNA was synthesized using a 5X All-In-One RT MasterMix (ABM, Canada). The relative quantification was done by ChamQTM Universal SYBR® qPCR Master Mix (Vazyme ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized by reverse transcription using All-In-One 5X RT Master Mix (ABM-G592) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of cDNA using 5× All-In- One RT MasterMix (Applied Biological Materials Inc.) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Some 500 ng of RNA were reverse transcribed using the ABM 5X RT MasterMix (Applied Biological Materials), as directed by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... RNA was reverse transcribed into first-strand cDNA using All-in-one RT MasterMix (Applied Biological Materials). qPCR of cDNA was then performed using P7/P8 primers (5249-5358) ...
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bioRxiv - Plant Biology 2022Quote: ... before reverse transcription to cDNA using a 5×All-In-One RT MasterMix (Applied Biological Materials Inc.). PhnsLTP1 ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription to generate cDNA was performed using the ABM All-In-One 5X RT MasterMix (ABM). Primer Express 3.0 software was used for designing primers for gene expression analysis by quantitative real-time polymerase chain reaction (Table S4) ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was synthesized from total RNA by using 5×All-In-One RT MasterMix (ABM, China) following the standard protocols ...
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bioRxiv - Microbiology 2023Quote: ... PCR reaction mixtures (25 μl) consisted of 12.5 μl of MegaFi Fidelity 2X PCR MasterMix (ABM Inc., Richmond, Canada), 1.5 μl of each primer (final concentration 0.6 μM) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was converted into cDNA using 5x ALL-In-One RT Master Mix (Applied Biological Materials Inc, G490) following the manufacturers’ instructions and diluted 20 times with nuclease-free H2O ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared by reverse transcription with All-in-One 5X RT MasterMix (G592, Applied Biological Materials Inc) with 1000ng RNA ...
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bioRxiv - Cell Biology 2019Quote: ... tested routinely by PCR (Applied Biological Materials, Canada) and ...
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bioRxiv - Microbiology 2022Quote: ... 1X Bestaq PCR buffer (Applied Biological Materials Inc.), dNTP mix containing 200 mM concentrations of each nucleotide (Promega ...
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bioRxiv - Genomics 2019Quote: ... The cDNA was prepared from 100-200 ng of total RNA using 5xAll-In-One RT Master mix (Applied Biological Materials Inc). Real-time PCR was performed using Fast SYBR Green Master Mix ...
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bioRxiv - Cell Biology 2022Quote: ... tested regularly for mycoplasma infection by PCR (ABM, Richmond, BC, Canada) and maintained in RPMI 1604 media supplemented with 10% FBS and 1% L-Glutamine in a 37°C incubator with 5% CO2.
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bioRxiv - Microbiology 2022Quote: ... 1X Bestaq PCR buffer (Applied Biological Materials Inc., Richmond, BC, Canada), dNTP nucleotide mix containing 200 mM concentrations of each nucleotide (Promega ...
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bioRxiv - Physiology 2020Quote: ... The cDNA was prepared from total RNA (50-100ng) with ABM 5x All-In-One RT Master Mix (G486, Applied Biological Materials Inc.). The cDNAs of SENP1 and cyclophilin A were amplified using preamplification primers and Platinum Taq DNA polymerase (10966-018 ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were routinely tested for Mycoplasma via PCR test (Applied Biological Materials).
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bioRxiv - Immunology 2021Quote: ... PCR products were visualized in agarose gel using a DNA fluorescent intercalator Safe-Green (ABM, USA) through electrophoresis.
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bioRxiv - Cell Biology 2019Quote: ... All cell lines were shown to be mycoplasma-free by DAPI staining and PCR test (ABM, G238).
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bioRxiv - Molecular Biology 2024Quote: ... and cells were passaged weekly and regularly tested for mycoplasma contamination by PCR-based assay (G238; ABM). To investigate the expression of miR-146a-5p under inflammatory stress ...
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bioRxiv - Biochemistry 2022Quote: Our parental HeLa cell line was authenticated by genomic sequencing of 24 loci (University of Utah Sequencing Core) and confirmed to be mycoplasma-free by routine PCR testing (ABM) following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using BrightGreen 2X qPCR MasterMix (ABM, Vancouver, Canada) on a QuantStudio 3 real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... the parental HeLa cell lines were authenticated by genomic sequencing of 24 loci (University of Utah Sequencing Core) and confirmed to be mycoplasma-free by routine PCR testing (ABM) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... and titered using lentivirus qPCR titer kit (ABM). 1963B and BT20 cells were transduced with the virus at an MOI of 10 following puromycin selection (2 ug/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNA generated (OneScript Hot cDNA Synthesis Kit, ABM) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma negativity of the original cell lines (hTERT RPE-1 and 293T) grown in antibiotics-free media were confirmed by a PCR based test (G238, Applied Biological Materials).
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma negativity of the original cell lines (hTERT RPE-1 and 293T) grown in antibiotics-free media were confirmed by a PCR based test (G238, Applied Biological Materials).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... carried out with OneScript® Plus cDNA Synthesis Kit (ABM), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... the PCR products and digested plasmids were fused into complete expression plasmids using the Ligation-Free Cloning System (Applied Biological Materials, Inc., Canada).
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bioRxiv - Bioengineering 2020Quote: ... by following the procedures described elsewhere.55 PCR products were purified using DNA purification solid-phase reversible immobilization magnetic beads (G95, Applied Biological Materials Inc.) and the purified products were sent out for Sanger sequencing (Genewiz Inc.).
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bioRxiv - Neuroscience 2022Quote: ... Restriction enzymes BamHI and EcoRI were used to digest the PCR product of SLC19a1 and expression plasmids (pLenti-EF1α-GFP-2A-Puro; Applied Biological Materials, Richmond, Canada). Subsequently ...
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bioRxiv - Microbiology 2020Quote: The OneScript Reverse Transcriptase cDNA Synthesis kit by Applied Biological Materials Inc ...