Labshake search
Citations for Bimake :
1 - 50 of 52 citations for 6 Methoxy 2 3 4 9 tetrahydro 1H b carbolin 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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bioRxiv - Cancer Biology 2023Quote: ... and phosphatase inhibitor mixture mixes A and B (100X) (Bimake). The lysates were cleared by centrifugation at 13,000 rpm at 4°C in a microcentrifuge for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μL of 1:10 diluted cDNA was added to 10 μL of 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA) and the final concentration of each primer was 250 nM in 20 μL of total volume ...
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bioRxiv - Cancer Biology 2022Quote: ... and reverse transcribed using the All-in-One cDNA Synthesis Kit (Bimake, B24403). qRT-PCR was performed using SYBR Green qPCR Master Mix (Bimake ...
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bioRxiv - Genomics 2020Quote: ... before reverse transcription using All-in-One cDNA Synthesis SuperMix (Bimake, Houston, TX, USA). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2020Quote: ... the reverse transcription (RT) reactions were performed using All-in-One cDNA Synthesis SuperMix kit (Bimake). The cDNA was then used for real-time qPCR using 2x SYBR Green qPCR Master Mix (Bimake ...
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bioRxiv - Biochemistry 2022Quote: Cells were lysed in RIPA buffer (Thermo) containing protease and phosphatase inhibitor cocktails (Bimake B14001 and B15001-B). Protein concentration was quantified by BCA assay (Thermo Fisher) ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... 1 mM tris(2-carboxyethyl)phosphine(TCEP) 10% (v/v) glycerol with additional EDTA-free Protease Inhibitor Cocktail (Bimake), and then incubated with agitation for 20 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... cell extracts (3 ml of the supernatant) were incubated with Anti-Flag Affinity Gel (Bimake) at 4 °C for 3 h ...
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bioRxiv - Neuroscience 2022Quote: ... 3 mM CaCl2 and 12 mM MgCl2) containing protease and phosphatase inhibitor cocktails (Bimake, Houston, TX), meninges and white matter were removed ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time PCR was performed on a QuantStudio 3 Real-time PCR system using gene-specific primers (Table S2) and 2x SYBR Green qPCR Master Mix (Bimake). The amplification conditions were 40 cycles of 95°C for 10 s and 60°C for 30 s ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 mM NaCl and 2 mM MgCl2 supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization ...
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bioRxiv - Immunology 2020Quote: ... The cDNA was mixed with 2 × SYBR Green qPCR Master Mix (B21202, Bimake, Houston, TX, USA), and the real-time PCR reaction was performed on the 7500 Real-Time PCR System (ABI ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed in triplicate or quadruplicate using 2 x SYBR Green qPCR Master Mix (Bimake.com) with specific primers and normalized to reference genes (Table 1).
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bioRxiv - Plant Biology 2021Quote: qRT-PCR was performed with 2X SYBR Green qRT-PCR Master Mix with Rox #2 (Bimake; Houston, TX, USA), as follows per well ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed by using the Agilent Mx3000P qPCR system with the 2×SYBR Green qPCR Master Mix (Bimake). Relative mRNA expression was determined by the delta cycle time with human GAPDH as the internal control in data normalization ...
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bioRxiv - Neuroscience 2020Quote: ... Real-time polymerase chain reaction was carried out in duplicate using 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA), and reactions were run on a CFX Connect Real-Time PCR Detection System (BioRad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Nphp1 cDNA fragments between exons 2 and 11 were PCR-amplified using Universe High-Fidelity Hot Start DNA polymerase (Bimake) and two primers (F1-mNPHP1-RT and R1-mNPHP1-RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Then the cDNA was subjected to quantitative RT-PCR (qRT-PCR) using the SYBR green assay with 2× SYBR green qPCR master mix (Bimake). Thermal profile was 95 °C for 5 min and 40 cycles of 95 °C for 15 sec and 60 °C for 20 sec ...
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bioRxiv - Genomics 2023Quote: ... brought the volume of RIPA to 1 mL and supplemented it with 1× PIC (Bimake, B14001). Subsequent incubation with antibodies ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... seeded in 96-well plates (2×103/well, five replicates) and cell viability was measured with Cell Counting Kit-8 (CCK-8) (Bimake.com, China). The CCK-8 test was repeated every 24 hrs for three days.
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bioRxiv - Microbiology 2023Quote: ... Infected cells were collected at 2 days post infection and washed three time with PBS supplemented with protease inhibitor cocktail (Bimake, B14001). The cell pellets were resuspended in lysis buffer (Beyotime ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... 1 × Protease Inhibitor Cocktail (Bimake; Cat#: B14001)) on ice for 15 min ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... and 1% phosphatase inhibitor cocktail (B15001, Bimake) at 4°C for 20 minutes ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... 1% EDTA-free protease inhibitor cocktail (B14001, Bimake) and 1% phosphatase inhibitor cocktail (B15001 ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... protease inhibitor cocktail (Bimake, 1 mL/ 100 mL suspension). The cell pellets were dounced to homogeneity and subsequently added with 1mM dopamine (MCE Inhibitors ...
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bioRxiv - Cancer Biology 2021Quote: ... containing proteinase inhibitor an phosphatase inhibitor (1/100; Bimake) by sonication using Branson Sonifier 150 with a duty cycle at 25% ...
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bioRxiv - Biochemistry 2022Quote: ... protease inhibitor cocktail (Bimake, 1 mL/ 100 mL suspension). The protein complex was assembled on membrane by adding 100 μM rotigotine (TargetMol ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Homogenates were centrifuged at 20,000 xg for 15 min at 4 °C to remove insoluble materials ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Homogenates were clarified by centrifugation at 20,000x g for 15 min at 4 °C and supernatants were collected ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100) with EDTA-free protease inhibitors (Bimake) and phosphatase inhibitors (Bimake ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100) supplemented with 1x protease inhibitor (Bimake.com) and SuperNuclease (Sino Biological) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Lysates were centrifuged at 20,000x g for 15 minutes at 4°C to precipitate insoluble materials.
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bioRxiv - Bioengineering 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on ice ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... anti-TUBULIN rabbit antibody (Bimake, Houston, TX, USA, A5105, 1:1000), and then detected with sheep anti-mouse IgG-HRP (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on a rotator at 4°C ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... 1% SDS) supplemented with protease inhibitor cocktail (Bimake, Cat. No. B14002) and phosphatase inhibitor cocktail (Bimake ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14002, SKU# B14002-1 mL x 10, 1 mL x 10, $270.00 Ask -
bioRxiv - Genetics 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on a rotator at 4°C ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Plant Biology 2019Quote: ... 1% (v/v) Protease Inhibitor Cocktail (Bimake, added immediately prior to use) was added to the frozen powder (2 ml per g fresh weight ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% NP-40 and 5% glycerol) supplemented with protease inhibitors (Bimake, Cat# B14012) and phosphatase inhibitors (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% w/v deoxycholic acid (sodium salt)) supplemented with protease inhibitors (Bimake, Cat# B14001) followed by sonication ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2022Quote: ... 1 mM MnCl2 and 100 μM TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM MnCl2 and 100 μM TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% Triton X-100) containing a cocktail of protease and phosphatase inhibitors (Bimake, Houston, TX), sonicated (3 × 45 s in a Sonic Dismembrator apparatus ...
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bioRxiv - Genomics 2019Quote: ... Cells were washed with cold PBS and incubated in 375 µL lysis buffer (10 mM Tris pH 7.5, 10 mM NaCl, 0.2% NP40, 1× protease inhibitors (Bimake), 37.5 U SUPERase.In RNase inhibitor (Invitrogene) ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then washed twice with cold PBS and resuspended in SDS lysis buffer (50 mM Tris– HCl, pH8.1, 10 mM EDTA, 0.5% SDS, 1×Protease inhibitor cocktail (Bimake)) ...
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bioRxiv - Genomics 2021Quote: Total proteins were extracted using the RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) containing 1×complete proteinase inhibitor (Bimake), and protein concentration was defined using BCA protein assay kit (Beyotime) ...
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bioRxiv - Genomics 2023Quote: ... The pellet was resuspended in 550 μL of ice cold ChIP lysis buffer (50 mM Tris-HCl pH 8.0, 1% SDS, 10 mM EDTA) supplemented with 0.5× PIC (Bimake, B14001) and 0.5 mM PMSF ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Genomics 2023Quote: ... Permeabilized cells were resuspended in 550 μL of ice cold ChIP lysis buffer (50 mM Tris-HCl pH 8.0, 1% SDS, 10 mM EDTA) supplemented with 0.5× PIC (Bimake, B14001) and 0.5 mM PMSF ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Immunology 2021Quote: ... Spleens and graft tissues were homogenized in SDS free RIPA buffer of pH 8.0 (10mM Tris HCL, 1mM EDTA, 140mM NaCl and 1% Triton X-100) supplemented with protease inhibitor cocktail (Bimake.com, Houston, TX) using a micro-tube homogenizer (Bel-Art ...