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1 - 50 of 189 citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020

• Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin

  Yisong Qian, et al., bioRxiv - Immunology 2021

  Quote: ... and the p38 inhibitor SB203580 (20 μM, Enzo Life Sciences, Farmingdale, NY) were added into HLMEC cultures 1 h before NP exposure ...
• Evolutionarily conserved chaperone-mediated proteasomal degradation of a disease-linked aspartoacylase variant

  Sarah K. Gersing, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-20S α-subunits (Enzo Life Sciences, MCP231 PW8195), anti-Hsp70 (Invitrogen ...
• HCV infection activates the proteasome via PA28γ acetylation and heptamerization to facilitate the degradation of RNF2, a catalytic component of polycomb repressive complex 1

  Hirotake Kasai, et al., bioRxiv - Microbiology 2024

  Quote: ... and proteasome activator 11S γ subunit (Enzo Life Science). The immunoprecipitates containing HA-RNF1 or HA-RNF2 (one-twentieth of the total immunoloprecipitates ...
• The C. elegans proteasome subunit RPN-12 is required for hermaphrodite germline sex determination and oocyte quality

  Lourds M. Fernando, et al., bioRxiv - Developmental Biology 2020

  Quote: ... a mouse monoclonal proteasome 20S core particle antibody that recognizes alpha 1,2,3,4,5,6,7 subunits (1:200, Enzo Life Sciences, Cat. No. BML-PW8195) and rabbit GFP antibody (1:500 ...
• Identification of a non-canonical function of prefoldin subunit 5 in proteasome assembly

  Somayeh Shahmoradi Ghahe, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-proteasome 20S core subunits (BML-PW9355; Enzo Life Sciences), anti-DNP antibody (MAB2223 ...
• Vimentin provides target search efficiency and mechanical resilience for dendritic cell migration

  Luiza Da Cunha Stankevicins, et al., bioRxiv - Biophysics 2020

  Quote: ... and an anti-biotin monoclonal antibody (Hsc70; 1B5; Enzo Life Sciences, Plymouth, PA, USA).
• The response network of HSP70 defines vulnerabilities in cancer cells with the inhibited proteasome

  Magdalena Oroń, et al., bioRxiv - Cancer Biology 2022

  Quote: ... NP40 1% and protease inhibitors [Thermo]) and immunoprecipitation was performed overnight at 4°C using Proteasome 20S core subunits polyclonal antibody (ENZO Life Sciences BML-PW8155) and IP buffer (137 mM NaCl ...
• A secreted helminth microRNA suppresses gastrointestinal cell differentiation required for innate immunity

  Matias G. Perez, et al., bioRxiv - Microbiology 2024

  Quote: ... and iii) selective p38 MAP kinase inhibitor: SB202190 (10 µM) (Enzo Life Science, ALX-270-268-M001) as previously reported by Smith et al.87 ...
• Selective germline genome edited pigs and their long immune tolerance in Non Human Primates

  Lijin Zou, et al., bioRxiv - Synthetic Biology 2020

  Quote: ... and biotin-conjugated isolectin B4 (Enzo Life Science, USA) using a magnetic field ...
• NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB

  Maurice Diwo, et al., bioRxiv - Microbiology 2020

  Quote: ... and the cytosolic control proteins DnaK (DnaK monoclonal antibody, Enzo Life Sciences).
• Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1

  Katarzyna Szymanska, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and mouse anti-mono- and polyubiquitinylated conjugates clone FK2 and rabbit anti-20S proteasome α7 subunit (Enzo Life Sciences Inc.). Rabbit anti-MKS1 has been described previously 51,52 ...
• Nix induced mitochondrial fission, mitophagy, and myocyte insulin resistance are abrogated by PKA phosphorylation

  Simone Cristina da Silva Rosa, et al., bioRxiv - Cell Biology 2020

  Quote: ... The Kemptide substrate (Enzo Life Sciences; #P-107; LRRASLG) was used as a positive control in each assay ...
• Misoprostol Treatment Prevents Hypoxia-Induced Cardiac Dysfunction Through a 14-3-3 and PKA regulatory motif on Bnip3

  Matthew D. Martens, et al., bioRxiv - Cell Biology 2021

  Quote: ... The Kemptide substrate (Enzo Life Sciences; #P-107; LRRASLG) was used as a positive control in each assay ...
• Cohesin is required for long-range enhancer action

  Lauren Kane, et al., bioRxiv - Genetics 2021

  Quote: ... 160-240 ng of biotin- and Green496-dUTP-labeled (Enzo Life Sciences) (2-colour ...
• High yield purification of an Isoleucine zipper modified CD95 Ligand with either biotin or DNA-oligomer binding domain for efficient Cell Apoptosis Induction

  Xiaoyue Shang, et al., bioRxiv - Cell Biology 2024

  Quote: ... CD95L-IZ-biotin or soluble CD95L (Enzo Life Sciences Inc., Lörrach, Germany) was diluted from 0.1 nM to 100 nM in 0.5 % BSA/PBST and incubated with CD95-His at r.t ...
• A claudin5-binding peptide enhances the permeability of the blood-brain-barrier

  Martina Trevisani, et al., bioRxiv - Neuroscience 2024

  Quote: ... and the housekeeping protein calnexin (rabbit polyclonal antibody, Enzo Life Sciences, ADI-SPA-860, 1:1000). After 3x washes with 0.05% TBS-T ...
• Cardiac myosin binding protein-C palmitoylation is associated with increased myofilament affinity, reduced myofilament Ca²⁺ sensitivity and is increased in ischaemic heart failure

  Alice Main, et al., bioRxiv - Cell Biology 2022

  Quote: ... p-cMyBP-C (1:1000, pSer282, Enzo Life Sciences, ALX-215-057-R050), myosin heavy chain (1:200 ...
• Epac activation reduces trans-endothelial migration of undifferentiated neuroblastoma cells and cellular differentiation with a CDK inhibitor further enhances Epac effect

  Rabiu Inuwa, Diana Moss, John Quayle, Rasha Swadi, bioRxiv - Cancer Biology 2024

  Quote: ... PKI (P 210) (myristoylated PK (14-22)) amide) was bought from Enzo Life Sciences Inc ...
• The association of human astrovirus with extracellular vesicles facilitates cell infection and protects the virus from neutralizing antibodies

  Baez-N Carlos, et al., bioRxiv - Microbiology 2021

  Quote: ... respectively, while monoclonal antibody specific to anti-protein disulfide isomerase (PDI, clone 1D3) was obtained from Enzo Life Sciences, Inc (C ...
• Knockdown of Atg1 or Atg18 in adult adipocytes impairs lipophagy and reduces lifespan of fruit flies (Drosophila melanogaster)

  Changqi Zhu, et al., bioRxiv - Genetics 2023

  Quote: ... Mouse monoclonal antibody anti-Mono- and poly-ubiquitinylated proteins (FK2) from Enzo Life Sciences (1:200 dilution was used), Alexa Fluor 488 anti-mouse IgG (H+L ...
• Reduced S-acylation of SQSTM1/p62 in Huntington disease is associated with impaired autophagy

  F. Abrar, et al., bioRxiv - Neuroscience 2023

  Quote: ... the protein was immunoprecipitated using the same antibody and detected with rabbit anti-SQSTM1 (Enzo Life Sciences, BML-PW9860). For HA-tagged proteins ...
• Epigenetic control and inheritance of rDNA arrays

  Tamara Potapova, et al., bioRxiv - Genomics 2024

  Quote: The PCR amplicon was labeled with Biotin-16-dUTP using the nick translation kit (Enzo Life Sciences) and detected with streptavidin conjugated to Cy5 (Thermo)
• Structure of Blm10:13S proteasome intermediate reveals parallel assembly pathways for the proteasome core particle

  Mandeep Kaur, et al., bioRxiv - Biochemistry 2024

  Quote: ... Proteins were separated on SDS-PAGE and Western blots were done using antibodies against Blm10 (Rabbit polyclonal; Enzo Life Sciences BML-PW0570), Pba1/2 17 ...
• Pyruvate dehydrogenase kinase expression profile is a biomarker for cancer sensitivity to dichloroacetate-mediated growth inhibition

  Bevan P Gang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 2004) were used to detect proteins of interest with the following primary antibodies: PDK1 (Cat# ADI-KAP-PK112, Enzo Life Sciences, Plymouth Meeting, PA, USA), PDK2 (Cat# AP7039b ...
• HuD (ELAVL4) gain-of-function impairs neuromuscular junctions and induces apoptosis in in vitro and in vivo models of amyotrophic lateral sclerosis

  Beatrice Silvestri, et al., bioRxiv - Neuroscience 2024

  Quote: ... to single cells and replated in Nutristem P/S supplemented with 10 µM Y-27632 ROCK inhibitor (Enzo Life Sciences). The next day differentiation was induced in DMEM/F12 (Dulbecco’s Modified Eagle’s Medium/ Nutrient Mixture F-12 Ham ...
• Neural-specific alterations in glycosphingolipid biosynthesis and cell signaling associated with two human ganglioside GM3 Synthase Deficiency variants

  Michelle Dookwah, et al., bioRxiv - Neuroscience 2021

  Quote: ... O-GlcNAcylation of proteins in cell lysates was detected by western blot with anti-O-GlcNAc monoclonal antibody RL-2 (Enzo Life Sciences, ALX-804-11-R100) at 1:1000 dilution with detection by goat anti-mouse secondary at 1:10000 ...
• Highly specific intracellular ubiquitination of a small molecule

  Weicheng Li, et al., bioRxiv - Biochemistry 2024

  Quote: ... purified 100 nM HA-tUI protein were mixing with 10 µM ubiquitin protein (Enzo life sciences) for 5 min at room temperature in blocking buffer before adding to the sample ...
• An early Shh-H₂O₂ feedback loop controls the progression of the regenerative program during adult zebrafish fin regeneration

  Marion Thauvin, et al., bioRxiv - Developmental Biology 2021

  Quote: ... An NAD(P)H oxidase inhibitor (Nox-i, #BML-El395-0010) was purchased from Enzo Life Sciences (Inc. Farmingdale, NY, USA). Cyclopamine (HH-i) ...
• The effect of estradiol during the early stages of osteoclast differentiation is associated with the accumulation of phosphorylated p53 in mitochondria and the inhibition of mitochondrial metabolism

  Adriana Marques-Carvalho, et al., bioRxiv - Cell Biology 2023

  Quote: ... 0.1% CHAPS and 100 μM caspase substrate (Ac-DEVD-p-nitroanilide (pNA) (ALX-260-03, Enzo Life Science, Farmingdale, NY, USA) for caspase 3 or Ac-LEHD-pNA (ALX-260-081 ...
• Cross-species analysis of ARPP19 phosphorylation during oocyte meiotic maturation charts the emergence of a new cAMP-dependent role in vertebrates

  Ferdinand Meneau, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... Recombinant proteins and OA (Enzo Life Sciences) were diluted in PBS and centrifuged at 14,000 rpm at 4°C for 5 minutes before injection ...
• The mitochondrial unfolded protein response promotes senescence in human microglia by increasing S-adenosylmethionine availability for polyamine synthesis

  Maria Jose Perez J., et al., bioRxiv - Neuroscience 2024

  Quote: Protein aggregation was measured with the PROTEOSTAT® Protein Aggregation Assay Kit (ENZ-51023-KP050, Enzo Life Sciences, Farmingdale, NY, USA) with modifications to the manufacturer’s protocol ...
• Age-associated failure of efficient cardiac proteostatic adaptations to chronic cAMP-stress is associated with accelerated heart aging

  Maria Grazia Perino, et al., bioRxiv - Cell Biology 2023

  Quote: The isolation of protein aggregates and the assessment of protein aggregation was conducted using the Proteostat kit (Enzo Life Science, ENZ-51023), as previously published14 ...
• Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4

  Michio Inoue, et al., bioRxiv - Neuroscience 2022

  Quote: ... and anti-ubiquitinylated proteins (Enzo Life Sciences, 1:100). The sections were probed in appropriate secondary antibodies ...
• Hsp70 chaperone blocks α-synuclein oligomer formation via a novel engagement mechanism

  Jiahui Tao, et al., bioRxiv - Neuroscience 2020

  Quote: ... and mouse monoclonal antibody G3.1 anti Hsp27 antibody (Enzo Life Science, Inc., Farmingdale, NY) were diluted at 1/1000 ...
• Restore mitophagy is essential to prevent cardiac oxidative stress during hypertrophy

  Victoriane Peugnet, et al., bioRxiv - Cell Biology 2021

  Quote: ... ubiquitinylated proteins (BML-PW8810-0500, Enzo Life Science, 1/2500). The horseradish peroxidase-labelled secondary antibodies used were ...
• HAP40 orchestrates huntingtin structure for differential interaction with polyglutamine expanded exon 1

  Rachel J Harding, et al., bioRxiv - Biochemistry 2021

  Quote: HTT protein samples were mixed with recombinant Caspase6 (Enzo Life Sciences) in a ratio of 100 U caspase6 to 1 pmol of HTT in 20 mM HEPES pH 7.4 ...
• Elucidating key determinants of engineered scFv antibody in MMP-9 binding using high throughput screening and machine learning

  Masoud Kalantar, et al., bioRxiv - Bioengineering 2024

  Quote: ... purchased His6x-MMP-9cd protein from Enzo Life Sciences (Farmingdale, NY) was used.
• Illuminating host-mycobacterial interactions with functional genomic screening to inhibit mycobacterial pathogenesis

  Yong Lai, et al., bioRxiv - Microbiology 2020

  Quote: ... vinculin monoclonal antibody (Enzo life sciences), and goat anti-mouse IgG HRP conjugate (Thermo Scientific) ...
• Pharmacological chaperones restore proteostasis of epilepsy-associated GABA_A receptor variants

  Ya-Juan Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... a potent protein synthesis inhibitor (Enzo Life Sciences, catalog #: ALX-380-269). Cells were then chased for the indicated time ...
• Correction of eIF2-dependent defects in brain protein synthesis, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Mauricio M. Oliveira, et al., bioRxiv - Neuroscience 2020

  Quote: ... 6E10 antibody was from Enzo Life Sciences. Biotin-conjugated anti-Iba1 was from Wako Fujifilm ...
• PARP12-catalyzed mono-ADP-ribosylation of Golgin-97 controls the transport of E-cadherin

  Giovanna Grimaldi, et al., bioRxiv - Cell Biology 2020

  Quote: ... Giantin-mouse monoclonal antibody (Enzo Life Sciences) was used at 1:1000 for immunofluorescence ...
• Activation loop phosphorylation and cGMP saturation of PKG regulate egress of malaria parasites

  Konstantinos Koussis, et al., bioRxiv - Microbiology 2024

  Quote: ... Polyclonal human PKG antibodies (Enzo Life Sciences) were used for PfPKG detection (1:1000 or 1:2000 ...
• Ubiquitination of stalled ribosomes enables mRNA decay via HBS-1 and NONU-1 in vivo

  Parissa C. Monem, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Anti-HA antibody (Enzo Life Sciences 16B12) was used at a 1:2000 dilution to detect HA-tagged RPS-10 protein ...
• Drosophila p38 MAPK Interacts with BAG-3/starvin to Regulate Age-dependent Protein Homeostasis

  Sarah M. Ryan, et al., bioRxiv - Genetics 2020

  Quote: ... Protein aggregates were identified using mouse anti-polyubiquitin 1:1000 (Enzo Life Sciences). Three muscles from each individual fly were imaged as z-series and flattened into a single image as a max projection using confocal microscopy for a total of 27 muscles per genotype ...
• Fibroblasts carrying intermediate C9orf72 hexanucleotide repeat expansions from iNPH patients show impaired energy metabolism but no cell pathologies

  Dorit Hoffmann, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-poly-ubiquitinated proteins (FK1, 1:1000, BML-PW8805-0500, Enzo Life Sciences), anti-TDP-43 (1:1000 ...
• The mitochondrial genome segregation system of T. brucei: Single p197 molecules connect the basal body with the outer membrane

  Salome Aeschlimann, et al., bioRxiv - Cell Biology 2022

  Quote: ... monoclonal mouse anti-HA antibody (Enzo Life Sciences AG ...
• The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation

  Suchitra Mohanty, et al., bioRxiv - Microbiology 2020

  Quote: ... Ubiquitin antibody was purchased from Enzo Life Sciences. DAPI (4’ ...
• p97/VCP induces GLI1 to control XBP1-dependent endoplasmic reticulum stress transcriptional response

  Kim Barroso, et al., bioRxiv - Cancer Biology 2021

  Quote: ... antibodies against KDEL were from Enzo life science (ADI SPA 827 for the detection of BiP/GRP78 and GRP94) ...
• Prolonged disturbance of proteostasis induces cellular senescence via temporal mitochondrial dysfunction and enhanced mitochondrial biogenesis in human fibroblasts

  Yasuhiro Takenaka, et al., bioRxiv - Cell Biology 2021

  Quote: Protein aggregation was visualized using ProteoStat Aggresome Detection Reagent (Enzo Life Science, Farmingdale, NY), according to the manufacturer’s protocol ...
• The C-type Lectin Receptor Dectin-2 is a receptor for Aspergillus fumigatus galactomannan

  Jennifer L. Reedy, et al., bioRxiv - Immunology 2022

  Quote: ... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...