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Citations for Enzo Life Sciences :
301 - 348 of 348 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Primary incubation took place overnight at 4 °C (anti-pSer282; 1:2,000, ENZO Life Sciences, AL-215-057-R050). Secondary antibody incubation was done at room temperature for 1h (HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2024Quote: ELISA was used to quantify HO-1 levels in rat midbrain samples (Enzo Life Sciences cat#ADI-EKS-810A) and human CSF samples (Abcam cat#ab207621) ...
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bioRxiv - Developmental Biology 2020Quote: ... a mouse monoclonal proteasome 20S core particle antibody that recognizes alpha 1,2,3,4,5,6,7 subunits (1:200, Enzo Life Sciences, Cat. No. BML-PW8195) and rabbit GFP antibody (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated overnight at 4°C in rabbit anti-amyloid beta antibody (1:200; clone 6E10, Enzo Life Sciences), followed by Alexa-488-labelled goat anti-rabbit secondary antibody (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... The antibody for detection of HO-1 (ADI-SPA-896) was purchased from Enzo life science (New York, NY, USA). The antibodies for GAPDH (ab8245) ...
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bioRxiv - Genetics 2021Quote: ... Oxygen consumption rates were calculated following injection of the following compounds: oligomycin (1 μg/mL) (Enzo Life Sciences, BML-CM111), carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Physiology 2022Quote: ... iPSC-cardiomyocytes were immunolabeled with a 1:200 dilution of the primary antibody #ALX-215-050-R100 (Enzo Life Sciences). To target the hERG1b isoform ...
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bioRxiv - Cell Biology 2019Quote: ... The antibodies used were 1:1000 mono- and poly-ubiquitinated conjugates monoclonal antibody (FK2) (Enzo Life Sciences Cat# BML-PW8810, RRID:AB_10541840); 1:10,000 Peroxidase AffiniPure Donkey Anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: Blocked blots were washed three times in 1 × TBST washes before incubation with pre-chilled mouse anti-DnaK antibody (1:10,000 Ab in 3 % BSA and 0.01 % sodium azide in TBST; Enzo Life Sciences, Inc.) for one hour ...
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bioRxiv - Cell Biology 2022Quote: ... Parallel sets of cultures were maintained in an identical growth medium with the addition of 1 nM Rapa (Enzo Life Sciences). Immortalized Li Fraumeni skin fibroblasts were cultured as described in Medcalf et al. ...
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bioRxiv - Immunology 2022Quote: ... The GM1/KDEL-ELISA plates were incubated with a primary mouse anti-KDEL mAb (10C3, Enzo Life Sciences; 1:1000 diluted) at 37 °C for 1 hr followed by the multi-species SP ads-HRP-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Substrate working solution was prepared by mixing 1% v/v of 10 mM Z-LR-AMC (BML-P229, Enzo Life Sciences) in DMSO (1.02952.1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and the rat monoclonal anti-GRP94 (clone 9G10) (catalog #: ADI-SPA-850-F, 1:1,000) antibodies were purchased from Enzo Life Sciences. The rabbit polyclonal anti-XBP1 antibody (M-186 ...
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bioRxiv - Biochemistry 2023Quote: ... and Z)-1-[2-(2-Aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA/NONOate) (ALX-430-014-M005, Enzo Life sciences) involved plating cells in 96-well plates at a final density of 1x106 cells/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Cumulative exocytosis was achieved by blocking SV reacidification by adding 1 μM bafilomycin A1 (Enzo Life Sciences, Cat#BML-CM110-0100) to the bathing medium itemized above ...
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bioRxiv - Biochemistry 2023Quote: ... CAS: 1001600-56-1) and zVAD-FMK was obtained from Enzo Life Sciences (Cat. # ALX-260-020; CAS: 220644-02-0). Propidium Iodide ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used for western blotting and immunoprecipitation experiments: Caspase8 (1:2000, Enzo Life Science, ALX-804-447-C100), MLKL (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... followed by neutralization with 1 vol 2M NaOH) and total phosphate was determined with the Biomol Green kit (Enzo Life Sciences). Results are then expressed as nmol phosphate normalized to the amount of bacterial cells expressed as OD at 595 nm.
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bioRxiv - Neuroscience 2021Quote: ... The other set of slices were stained with mouse anti-HSP70/HSP72 (ADI-SPA-810-D; Enzo Life Sciences; 1:400 dilution), and rabbit anti-cleaved caspase-3 (Asp175 ...
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bioRxiv - Microbiology 2019Quote: ... and immunoblotted with the following antibodies using standard procedures: mouse monoclonal anti-HLA-DRA (1:1000; Clone: 302CT2, Enzo Life Sciences, UK) or rabbit monoclonal a-tubulin (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... Transduced mice from the experimental group received an intraperitoneal injection of CNO (dissolved in sterile saline to a dose of 1 mg/kg; catalog: BML-NS105, Enzo Life Sciences) 30 min before each session of the conditioning ...
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bioRxiv - Cell Biology 2021Quote: ... pH=7.6) as well as a standard curve of purified 26S Proteasome (0.5, 1 and 10 g/L; Enzo Life Sciences Inc., USA) were included in each assay ...
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bioRxiv - Cell Biology 2022Quote: ... To target the hERG1 C-terminal domain we immunolabeled cardiomyocytes with a 1:150 dilution of primary antibody #ALX-215-049-R100 from Enzo Life Sciences and 1:250 dilution of secondary antibody AF647 ...
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bioRxiv - Microbiology 2022Quote: ... overnight cultures were adjusted to 1 OD600nm by diluting the suspensions with PBS and treated with anti-K1 rabbit antibody (ENZ-ABS559-0100 Enzo Life Sciences) (1/100 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Oocytes were collected at metaphase II and K71M-Gwl was recovered by incubating the oocyte extract with Nickel beads in the presence of 1 μM OA (Enzo Life Sciences). Nickel beads were washed in kinase buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated ON at 4 °C in blocking solution with the primary antibodies: rabbit polyclonal anti-ubiquitin antibody (1:300; ADI-SPA-200-F, Enzo Life Sciences), rabbit anti-dopamine and cyclic AMP-regulated neuronal phosphoprotein 32 (DARPP-32 ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... 4 °C) with primary antibodies diluted in the DuoLink® Antibody Diluent: rabbit anti-HSP90 (1:200; #ADI-SPA-836, Enzo Life Science) or rabbit anti-HSF1 (1:300 ...
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bioRxiv - Neuroscience 2019Quote: ... Antibodies were diluted in PBST and used at the following concentrations: rabbit anti-CSPα 1:20,000 (Enzo Life Sciences Cat# VAP-SV003E, RRID:AB_2095057); mouse anti-dCSP ...
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bioRxiv - Neuroscience 2022Quote: ... N5-(1-Imino-3-butenyl)-L-ornithine (L-VNIO, 100 nM) was from Enzo Life Science (ALX-270-216-M005, Farmington, NY, USA). KT-5823 (500 nm ...
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bioRxiv - Biochemistry 2020Quote: 10 µM Rab1b was incubated with 0.1 µM AnkX in the presence of 1 mM CDP-choline (Enzo Life Sciences, Farmingdale, New York) for 2 h at 23 °C as published before (Goody et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... non-proteinuric pregnant women groups by sandwich ELISA (sFlt-1 and VEGF ELISA kit: R&D Systems Inc., Minneapolis, MN, U.S.A., GRP 78: Enzo Life Sciences, Inc.).
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bioRxiv - Microbiology 2023Quote: ... blocked in PBS 3% milk and labelled with primary antibodies diluted in blocking buffer overnight at 4 □C in humid chamber (mouse α-conjugated ubiquitin - 1:100: BML-PW8810-0100 - Enzo life science, mouse α-p62 - 1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... and then Kv2.1 expression was induced in Kv2.1-CHO cells with 1 μg/ml minocycline (Enzo Life Sciences, cat. #ALX-380-109-M050;), prepared in 70% ethanol at 2 mg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Equal loading and transfer of proteins was verified with a reversible protein stain (Ponceaus S) and by re-probing membranes with a housekeeping protein calnexin (1:20,000, #ADI-SPA-860, Enzo Life Sciences, Farmingdale, NY, USA). Integrated band density of each protein sample was measured using Image Studio Lite software (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Oxygen consumption rates were measured following sequential injection of the following compounds into each well: oligomycin (1□μg/mL) (Enzo Life Sciences, BML-CM111), carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were used in the following concentrations: mouse monoclonal antibody (mAb) anti-α-Bassoon (Enzo Sap7F407, Enzo Life Sciences, Lörrach, Germany, 1:500), rabbit polyclonal antibody anti-α-Homer 1 (Synaptic Systems ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pre-treated for 1 h with 10 μM VAS-2870 (NOX-i; #BML-El395-0010, Enzo Life Sciences, Inc.; Farmingdale, NY, USA) or an equivalent amount of DMSO as a control ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... collected from COS-7 cells was added to the media (1:10) or 500 nM of SAG (Enzo Life Sciences, ALX-270-426-M001) dissolved in DMSO was added to the media ...
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bioRxiv - Neuroscience 2023Quote: Clozapine N-oxide (CNO; made 1 mg/ml in vehicle, given as 10 ml/kg for final dose of 10 mg/kg; Enzo Life Sciences, Farmingdale, NY) or vehicle (0.5% dimethyl sulfoxide ...
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bioRxiv - Biochemistry 2023Quote: ... Tardigrades were incubated for 1 h with a working concentration of 100 uM of all inhibitors from the SCREENWELL Redox Library (Enzo Life Sciences, Farmingdale, NY) and monitored for physiological changes ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-calnexin (1:1,000; ADI-SPA-865; Enzo Life Sciences, Farmingdale, NY, USA), mouse anti-calreticulin (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mM DTT for 10 min (Karademir et al., 2018) and then with fluorogenic substrates Suc-LLVY-AMC (Enzo Life Sciences, BML-P802-0005), Z-LLE-AMC (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... We normalized the expression of EAAT2 to the expression of primary rabbit anti-calnexin (ABI-SPA-860, Enzo Life Sciences Inc., NY, USA, 1:10000). Blots were incubated with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:10000 ...
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bioRxiv - Cell Biology 2021Quote: ... the last 20 min of antibody labeling was carried out together with DNA labeling using NUCLEAR-ID red DNA stain at 1:250 dilution (Enzo Life Sciences, Cat#ENZ-52406). For microscopy ...
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bioRxiv - Cell Biology 2020Quote: ... the following compounds and concentrations (DMSO stock solutions dissolved in EM buffer) were applied: 1 µM Cytochalasin D (Enzo Life Sciences Inc, #BML-T109-0001), 10 µM SMIFH2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated over night at 4°C with primary mouse mono- and polyubiquitinylated conjugates FK2 monoclonal antibody (1/500; Enzo Life Sciences Cat# BML-PW8810, RRID:AB_10541840) in 2,5% BSA in PBST ...
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bioRxiv - Neuroscience 2020Quote: Cultures were treated with pre-warmed 37°C serum-free media containing a 1:1000 dilution of DMSO for vehicle control or 20 μM SP600125 pan-JNK inhibitor (Enzo Life Sciences BML-EI305-0010; Farmingdale, NY, USA) and immediately transferred to a Zeiss 710 Confocal Microscope with stable environmental controls maintained at 37°C with 5% humidified CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate (input) was incubated with anti-HA or anti-cMyc antibodies (9E10; Santa Cruz Biotechnology, Dallas, TX, USA / 16B12 Enzo Life Sciences, Farmingdale NY, USA; dilution 1:40) in combination with 50 μl Protein G Dynabeads (Thermo Fischer Scientific ...