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1 - 50 of 151 citations for Gamma secretase subunit APH 1B APH1B Antibody FITC since 2020

• Evolutionarily conserved chaperone-mediated proteasomal degradation of a disease-linked aspartoacylase variant

  Sarah K. Gersing, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-20S α-subunits (Enzo Life Sciences, MCP231 PW8195), anti-Hsp70 (Invitrogen ...
• HCV infection activates the proteasome via PA28γ acetylation and heptamerization to facilitate the degradation of RNF2, a catalytic component of polycomb repressive complex 1

  Hirotake Kasai, et al., bioRxiv - Microbiology 2024

  Quote: ... and proteasome activator 11S γ subunit (Enzo Life Science). The immunoprecipitates containing HA-RNF1 or HA-RNF2 (one-twentieth of the total immunoloprecipitates ...
• Giving it a fair shake: A simple, fast, & efficient method to extract amatoxins from the death cap mushroom, Amanita phalloides

  Catharine A. Adams, et al., bioRxiv - Microbiology 2024

  Quote: ... and gamma-amanitin (≥90%, Enzo Life Sciences, Farmingdale, NY, USA) standards in LCMS-grade water were assessed ...
• The C. elegans proteasome subunit RPN-12 is required for hermaphrodite germline sex determination and oocyte quality

  Lourds M. Fernando, et al., bioRxiv - Developmental Biology 2020

  Quote: ... a mouse monoclonal proteasome 20S core particle antibody that recognizes alpha 1,2,3,4,5,6,7 subunits (1:200, Enzo Life Sciences, Cat. No. BML-PW8195) and rabbit GFP antibody (1:500 ...
• Identification of a non-canonical function of prefoldin subunit 5 in proteasome assembly

  Somayeh Shahmoradi Ghahe, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-proteasome 20S core subunits (BML-PW9355; Enzo Life Sciences), anti-DNP antibody (MAB2223 ...
• The response network of HSP70 defines vulnerabilities in cancer cells with the inhibited proteasome

  Magdalena Oroń, et al., bioRxiv - Cancer Biology 2022

  Quote: ... NP40 1% and protease inhibitors [Thermo]) and immunoprecipitation was performed overnight at 4°C using Proteasome 20S core subunits polyclonal antibody (ENZO Life Sciences BML-PW8155) and IP buffer (137 mM NaCl ...
• Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1

  Katarzyna Szymanska, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and mouse anti-mono- and polyubiquitinylated conjugates clone FK2 and rabbit anti-20S proteasome α7 subunit (Enzo Life Sciences Inc.). Rabbit anti-MKS1 has been described previously 51,52 ...
• A mitochondrial SET domain protein is essential in T. brucei and required for mitochondrial morphology and function

  Emily W. Knight, et al., bioRxiv - Microbiology 2024

  Quote: ... 2.5mM CaCl2) and 2 μL Annexin V-FITC and 2 μL propidium iodide (Enzo Life Sciences) and incubated for 15 min ...
• Nanoscale details of mitochondrial fission revealed by cryo-electron tomography

  Shrawan Kumar Mageswaran, et al., bioRxiv - Cell Biology 2021

  Quote: ... cells were treated with FITC-phalloidin (Cat No - ALX-350-268-MC01, Enzo Life Sciences, Ann Arbor, USA). Briefly ...
• DCBLD1 modulates angiogenesis by regulating of the VEGFR-2 endocytosis in endothelial cells

  Qi Feng, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The transparent retina was incubated with the FITC-conjugated Isolectin-B4 (20 μg/mL, Enzo Life Science, Farmingdale, NY, USA). Using the Biologic CMM Analyzer software (French College) ...
• Hsp70 chaperone blocks α-synuclein oligomer formation via a novel engagement mechanism

  Jiahui Tao, et al., bioRxiv - Neuroscience 2020

  Quote: ... and mouse monoclonal antibody G3.1 anti Hsp27 antibody (Enzo Life Science, Inc., Farmingdale, NY) were diluted at 1/1000 ...
• Illuminating host-mycobacterial interactions with functional genomic screening to inhibit mycobacterial pathogenesis

  Yong Lai, et al., bioRxiv - Microbiology 2020

  Quote: ... vinculin monoclonal antibody (Enzo life sciences), and goat anti-mouse IgG HRP conjugate (Thermo Scientific) ...
• Correction of eIF2-dependent defects in brain protein synthesis, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Mauricio M. Oliveira, et al., bioRxiv - Neuroscience 2020

  Quote: ... 6E10 antibody was from Enzo Life Sciences. Biotin-conjugated anti-Iba1 was from Wako Fujifilm ...
• PARP12-catalyzed mono-ADP-ribosylation of Golgin-97 controls the transport of E-cadherin

  Giovanna Grimaldi, et al., bioRxiv - Cell Biology 2020

  Quote: ... Giantin-mouse monoclonal antibody (Enzo Life Sciences) was used at 1:1000 for immunofluorescence ...
• Activation loop phosphorylation and cGMP saturation of PKG regulate egress of malaria parasites

  Konstantinos Koussis, et al., bioRxiv - Microbiology 2024

  Quote: ... Polyclonal human PKG antibodies (Enzo Life Sciences) were used for PfPKG detection (1:1000 or 1:2000 ...
• Ubiquitination of stalled ribosomes enables mRNA decay via HBS-1 and NONU-1 in vivo

  Parissa C. Monem, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Anti-HA antibody (Enzo Life Sciences 16B12) was used at a 1:2000 dilution to detect HA-tagged RPS-10 protein ...
• The mitochondrial genome segregation system of T. brucei: Single p197 molecules connect the basal body with the outer membrane

  Salome Aeschlimann, et al., bioRxiv - Cell Biology 2022

  Quote: ... monoclonal mouse anti-HA antibody (Enzo Life Sciences AG ...
• The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation

  Suchitra Mohanty, et al., bioRxiv - Microbiology 2020

  Quote: ... Ubiquitin antibody was purchased from Enzo Life Sciences. DAPI (4’ ...
• p97/VCP induces GLI1 to control XBP1-dependent endoplasmic reticulum stress transcriptional response

  Kim Barroso, et al., bioRxiv - Cancer Biology 2021

  Quote: ... antibodies against KDEL were from Enzo life science (ADI SPA 827 for the detection of BiP/GRP78 and GRP94) ...
• Increases in ambient temperature progressively disassemble Arabidopsis phytochrome B from individual photobodies with distinct thermostabilities

  Joseph Hahm, Keunhwa Kim, Yongjian Qiu, Meng Chen, bioRxiv - Plant Biology 2020

  Quote: ... including monoclonal mouse anti-phyB antibodies (gift from Akira Nagatani) and polyclonal rabbit anti-RPN6 antibodies (Enzo Life Sciences, BML-PW8370-0100) were used at 1:1000 dilution ...
• Nuclear aging in polyglutamine-induced neurodegeneration

  Dina Pereira, et al., bioRxiv - Neuroscience 2023

  Quote: ... sections were incubated ON at 4 °C in blocking solution with the primary antibodies: rabbit polyclonal anti-ubiquitin antibody (1:300; ADI-SPA-200-F, Enzo Life Sciences), rabbit anti-dopamine and cyclic AMP-regulated neuronal phosphoprotein 32 (DARPP-32 ...
• A TRAF-like E3 ubiquitin ligase TrafE coordinates endolysosomal damage response and cell-autonomous immunity to Mycobacterium marinum

  Lyudmil Raykov, et al., bioRxiv - Microbiology 2022

  Quote: ... anti-Ub (FK2) mouse monoclonal antibody (Enzo Life Sciences), anti-K63-linkage-specific mouse monoclonal antibody (Enzo Life Sciences) ...
• Incorporation of 5 methylcytidine alleviates innate immune response to self-amplifying RNA vaccine

  Mai Komori, et al., bioRxiv - Immunology 2023

  Quote: ... a mouse anti-RIG-I antibody (Enzo Life Sciences) or a rabbit anti-MDA5 antibody (Proteintech) ...
• Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancer

  Natalia Vydra, et al., bioRxiv - Cancer Biology 2021

  Quote: ... 4 °C) with primary antibodies diluted in the DuoLink® Antibody Diluent: rabbit anti-HSP90 (1:200; #ADI-SPA-836, Enzo Life Science) or rabbit anti-HSF1 (1:300 ...
• The UPR^mt Preserves Mitochondrial Import to Extend Lifespan

  Nan Xin, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-HSP-6 antibody (Enzo Life Science ADI-SPS-825); and anti-NDUF3 [17D95] antibody (Abcam ab14711) ...
• Direct phosphorylation of HY5 by SPA1 kinase to regulate photomorphogenesis in Arabidopsis

  Wenli Wang, et al., bioRxiv - Plant Biology 2020

  Quote: ... anti-Tubulin antibodies (Enzo Life Sciences, Cat. # BML-PW8770-0025). Secondary HRP bound antibodies were visualized with Super Signal West Pico Chemiluminescent substrate (Pierce Biotechnology Inc. ...
• Breaking antimicrobial resistance by disrupting extracytoplasmic protein folding

  R. Christopher D. Furniss, et al., bioRxiv - Microbiology 2021

  Quote: ... and mouse anti-DnaK 8E2/2 antibody (Enzo Life Sciences) (dilution 1:10,000 in 5% w/v skimmed milk/TBS-T) ...
• Function of the bacteriophage P2 baseplate central spike Apex domain in the infection process

  John-Mark Miller, et al., bioRxiv - Molecular Biology 2023

  Quote: ... GroEL polyclonal antibody (Enzo Life Sciences, ADI-SPS-875-D), OmpF antibody (orb308741 ...
• Antibiotic potentiation and inhibition of cross-resistance in pathogens associated with cystic fibrosis

  Nikol Kadeřábková, et al., bioRxiv - Microbiology 2023

  Quote: ... and mouse anti-DnaK 8E2/2 antibody (Enzo Life Sciences) (dilution 1:10,000 in 5% w/v skimmed milk/TBS-T) ...
• A TRAF-like E3 ubiquitin ligase TrafE coordinates endolysosomal damage response and cell-autonomous immunity to Mycobacterium marinum

  Lyudmil Raykov, et al., bioRxiv - Microbiology 2022

  Quote: ... anti-K63-linkage-specific mouse monoclonal antibody (Enzo Life Sciences). Nuclei were stained with 1 μM DAPI (Invitrogen) ...
• Evasion of APOBEC1-mediated Intrinsic Immunity by a Herpesvirus Uracil DNA Glycosylase Is a Determinant of Viral Encephalitis

  Akihisa Kato, et al., bioRxiv - Microbiology 2023

  Quote: ... and rat monoclonal antibody to Hsc70 (1B5; Enzo Life Science). Mouse polyclonal antibody to vUNG and rabbit polyclonal antibodies to UL12 and ICP22 were reported previously18 ...
• Tripartite interactions of PKA catalytic subunit and C-terminal domains of cardiac Ca²⁺ channel modulate its β-adrenergic regulation

  Shimrit Oz, et al., bioRxiv - Biochemistry 2023

  Quote: ... Antibodies used: mouse anti-Hsp90 (Enzo Life Science, #SPA-830); mouse anti-GFP Tag (Thermo Fisher Scientific ...
• Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus

  Qiwang Xiang, et al., bioRxiv - Molecular Biology 2024

  Quote: ... K63-linkage-specific antibody (Enzo Life Sciences, BML-PW0600-0100); K48-linkage-specific antibody (Cell Signaling Technology ...
• IL8 Drives an Elaborate Signal Transduction Process for CD38 to Produce NAADP from NAAD and NADP⁺ in Endolysosomes to Effect Cell Migration

  Tae-Sik Nam, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-goat or anti-mouse antibodies (1:5000; Enzo Life Sciences) in the blocking buffer at room temperature for 2 h ...
• Tryptophan residues in TDP-43 and SOD1 mediate the cross-seeding and toxicity of SOD1

  Edward Pokrishevsky, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-SOD1-100 antibody (#ADI-SOD-100, Enzo Life Sciences), mouse anti-HA antibody (#ab130275 ...
• Epac1 regulates cellular SUMOylation by promoting the formation of SUMO-activating nuclear condensates

  Wenli Yang, et al., bioRxiv - Cell Biology 2022

  Quote: ... or anti-SUMO1 antibody (Enzo Life Sciences, Inc., Cat.# MBL-PW8330). For the UBA2 or UBC9 thioester bond formation assay ...
• Loss of Spindly sensitizes EML4-ALK v3 lung cancer cells to HSP90 inhibitors

  Marco P. Licciardello, et al., bioRxiv - Cancer Biology 2022

  Quote: ... HSP72 (ADI-SPA-810) antibody was purchased from Enzo Life Sciences. Spindly antibody (CSB-PA842662LA01HU ...
• Branched ubiquitin chain binding and deubiquitination by UCH37 facilitate proteasome clearance of stress-induced inclusions

  Aixin Song, et al., bioRxiv - Biochemistry 2021

  Quote: ... Primary antibodies used include: RPT6 (Enzo Life Sciences PW9265, 1:250); K48-specific ubiquitin (Millipore 05-1307 ...
• Heme oxygenase-2 (HO-2) binds and buffers labile heme, which is largely oxidized, in human embryonic kidney cells

  David A. Hanna, et al., bioRxiv - Cell Biology 2021

  Quote: ... rabbit polyclonal HO-1 antibody (Enzo Life Sciences BML-HC3001-0025); mouse monoclonal calnexin antibody (Invitrogen MA3-027) ...
• PIF4/HEMERA-mediated daytime thermosensory growth requires the Mediator subunit MED14

  Abhishesh Bajracharya, et al., bioRxiv - Plant Biology 2022

  Quote: ... and polyclonal rabbit anti-RPN6 antibodies (Enzo Life Sciences, BMLPW8370-0100) were used at 1:1000 dilution ...
• Perturbation of de novo lipogenesis hinders MERS-CoV assembly and release, but not the biogenesis of viral replication organelles

  M. Soultsioti, et al., bioRxiv - Microbiology 2024

  Quote: ... mouse monoclonal anti-PDI antibody (Enzo Life Sciences, ADI-SPA-891), mouse monoclonal ERGIC-53 G1/93 Ab (Enzo Life Sciences/ Alexis Biochemicals ...
• Impaired maturation of wild-type superoxide dismutase 1 associated with neurodegeneration in Parkinson disease brain and a novel murine model

  Amr H. Abdeen, et al., bioRxiv - Neuroscience 2024

  Quote: ... were conjugated to 100 µg polyclonal SOD1 antibody (Enzo Life Sciences, Farmingdale ...
• Molecular basis of autoimmune disease protection by MDA5 variants

  Rahul Singh, et al., bioRxiv - Biochemistry 2024

  Quote: ... or anti-MDA5 antibody (Enzo Life Sciences, RRID:AB_2893162, 1:1000 dilution).
• Lysine methylation shields an intracellular pathogen from ubiquitylation

  Patrik Engström, et al., bioRxiv - Microbiology 2020

  Quote: ... fixed cells were permeabilized with 0.2% Triton-X for 5 min and then stained with the anti-Rickettsia I7205 antibody (1:500 dilution; gift from Ted Hackstadt) and anti-polyubiquitin FK1 antibody (Enzo Life Sciences, BML-PW8805-0500; 1:250 dilution), followed by Alexa 488 anti-rabbit antibody (Invitrogen ...
• A genetic compensatory mechanism regulated by c-Jun and Mef2d modulates the expression of distinct class IIa HDACs to ensure peripheral nerve myelination and repair

  Sergio Velasco-Aviles, et al., bioRxiv - Cell Biology 2021

  Quote: Antibodies used for western blotting: calnexin (Enzo Life Sciences, rabbit 1:1000), c-Jun (Cell Signaling Technology ...
• NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB

  Maurice Diwo, et al., bioRxiv - Microbiology 2020

  Quote: ... and the cytosolic control proteins DnaK (DnaK monoclonal antibody, Enzo Life Sciences).
• Nutrient copper signaling promotes protein turnover by allosteric activation of ubiquitin E2D conjugases

  C.M. Opazo, et al., bioRxiv - Cell Biology 2021

  Quote: ... a mouse monoclonal antibody (diluted 1:500; FK1, Enzo Life Sciences, USA) or a mouse monoclonal antibody (diluted 1:500 ...
• Chaperone Requirements for De Novo Folding of Saccharomyces cerevisiae Septins

  Daniel Hassell, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Primary antibodies: anti-GroEL (Enzo Life Sciences #ADI-SPS-870-D, RRID:AB_2039163), anti-DnaK (Enzo Life Sciences #ADI-SPA-880-F ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... The primary antibodies used (Bassoon, #ADI-VAM-PS003-F, Enzo Life Sciences GmbH ...
• Polyubiquitinated PCNA promotes alternative lengthening of telomeres by inducing break-induced replication

  Sangin Kim, et al., bioRxiv - Cell Biology 2023

  Quote: ... the blot was incubated with HRP-conjugated secondary antibodies (Enzo Life Sciences) for 30 min ...