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101 - 150 of 354 citations for Dengue Virus Serotype 1 Envelope Protein Human Fc Tag HEK293 since 2019

• Novel Mechanisms of Strigolactone-Induced DWARF14 Degradation in Arabidopsis thaliana

  Elena Sánchez Martín-Fontecha, et al., bioRxiv - Plant Biology 2024

  Quote: ... α-ubiquitin (1:500, Enzo Life Sciences) or α-actin (1:1000 ...
• Inhibition of the ubiquitin-proteasome system by a bioactivatable compound

  Tatiana A. Giovannucci, et al., bioRxiv - Cell Biology 2021

  Quote: ... 10 μg protein were mixed with 80 μl reaction buffer (lysis buffer without Triton X-100) and 10 μl ubiquitin-AMC (Enzo Life Sciences, BML-SE211-0025) for a final concentration of 1 μM ...
• Pyruvate dehydrogenase kinase expression profile is a biomarker for cancer sensitivity to dichloroacetate-mediated growth inhibition

  Bevan P Gang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 2004) were used to detect proteins of interest with the following primary antibodies: PDK1 (Cat# ADI-KAP-PK112, Enzo Life Sciences, Plymouth Meeting, PA, USA), PDK2 (Cat# AP7039b ...
• Loss of β-ketoacyl acyl carrier protein synthase III activity restores multidrug-resistant Escherichia coli sensitivity to previously ineffective antibiotics

  Yaoqin Hong, et al., bioRxiv - Microbiology 2022

  Quote: ... Immunoblot analyses were performed with rabbit α-KPC2 (1:8,000) and mouse α-DnaK (1:10,000, Enzo Life Sciences, kindly gifted by Associate Professor Renato Morona) ...
• An Aryl Hydrocarbon Receptor from the Caecilian Gymnopis multiplicata Suggests Low Dioxin Affinity in the Ancestor of All Three Amphibian Orders

  Sarah A. Kazzaz, et al., bioRxiv - Pharmacology and Toxicology 2019

  Quote: ... Blots were probed with a 1:1000 dilution of a monoclonal antibody SA210 (Enzo Life Sciences; 1 mg/mL), directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich) ...
• Neural-specific alterations in glycosphingolipid biosynthesis and cell signaling associated with two human ganglioside GM3 Synthase Deficiency variants

  Michelle Dookwah, et al., bioRxiv - Neuroscience 2021

  Quote: ... O-GlcNAcylation of proteins in cell lysates was detected by western blot with anti-O-GlcNAc monoclonal antibody RL-2 (Enzo Life Sciences, ALX-804-11-R100) at 1:1000 dilution with detection by goat anti-mouse secondary at 1:10000 ...
• A comprehensive atlas of the aging vertebrate brain reveals signatures of progressive proteostasis dysfunction

  Domenico Di Fraia, et al., bioRxiv - Systems Biology 2023

  Quote: ... Specific proteasome activity was determined as the difference between the total activity of protein extracts and the remaining activity in the presence of 20 μΜ MG132 (Enzo Life Sciences, BML-PI102-0005). Fluorescence was measured by multiple reads for 60 min at 37°C by TECAN Kinetic Analysis (excitation 380 nm ...
• mTORC1 and JUN are activated after deletion of Prohibitin 1 in Schwann cells and may link mitochondrial dysfunction to demyelination

  Gustavo Della-Flora Nunes, et al., bioRxiv - Neuroscience 2020

  Quote: ... p62 1:500 (Enzo Life Sciences #BML-PW9860). GAPDH or β-tubulin were used as loading controls ...
• NLRP3 controls ATM activation in response to DNA damage

  Mélanie Bodnar-Wachtel, et al., bioRxiv - Cancer Biology 2020

  Quote: ... anti-ASC (1:2000) from ENZO Life Science, anti-γH2AX (JBW301 ...
• Evolutionarily conserved chaperone-mediated proteasomal degradation of a disease-linked aspartoacylase variant

  Sarah K. Gersing, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-Rpn1 (Enzo Life Sciences, p112-1 PW9270), anti-20S α-subunits (Enzo Life Sciences ...
• Autophagy in T cells from aged donors is maintained by spermidine, and correlates with function and vaccine responses

  Ghada Alsaleh, et al., bioRxiv - Physiology 2020

  Quote: ... or 1 mM difluoromethylornithine (DFMO, Enzo Life Sciences) or 10 μM GC7 (or as indicated ...
• ECPAS/Ecm29-Mediated 26S Proteasome Disassembly is an Adaptive Response to Glucose Starvation

  Won Hoon Choi, et al., bioRxiv - Biochemistry 2022

  Quote: ... anti-PSMA4 (PW8115, Enzo Life Science, 1/5,000), anti-PSMA5 (PA5-17295 ...
• Forkhead box R1-mediated stress response linked to a case of human microcephaly and brain atrophy

  Andressa Mota, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HSPA1A and HSPA6 (Enzo life sciences, 1:1000), DHRS2 (Abcam ...
• DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway

  Raffaella Gallo, Arpan Rai, Lucas Pelkmans, bioRxiv - Cell Biology 2020

  Quote: ... ERGIC53 (1:400, Enzo Life Sciences, ABS300-0100), G3BP1 (1:400 ...
• Mistargeting of hydrophobic mitochondrial proteins activates a nucleus-mediated posttranscriptional quality control pathway in trypanosomes

  Caroline E. Dewar, et al., bioRxiv - Biochemistry 2021

  Quote: ... mouse HA (Enzo Life Sciences AG; 1:5000), rabbit anti-Ub (Proteintech ...
• Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4

  Michio Inoue, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti- HSP70/HSP72 (Enzo Life sciences, 1:1000), anti-p62 (Proteintech ...
• Early nuclear phenotypes and reactive transformation in human iPSC-derived astrocytes from ALS patients with SOD1 mutations

  Vincent Soubannier, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-SOD1 (1/500; Enzo Life Sciences; Farmingdale ...
• Proteostatic remodeling of small heat shock chaperones - crystallins by Ran-binding protein 2 and the peptidyl-prolyl cis-trans isomerase and chaperone activities of its cyclophilin domain

  Hemangi Patil, Kyoung-in Cho, Paulo A. Ferreira, bioRxiv - Genetics 2024

  Quote: Rabbit anti-hsc70 (1:3,000, ENZO Life Science, Farmingdale ...
• Extracellular Matrix Regulates Neuronal Chloride Concentration via K⁺-Cl⁻-Cotransporter 2

  Tushar Devanand Yelhekar, et al., bioRxiv - Neuroscience 2023

  Quote: ... the proteins were separated by SDS‐PAGE on 7.5 % gels and immunoblotted with anti‐ KCC2 (1:1000) and anti‐calnexin antibody (1:2000; Enzo Life Sciences). Densitometric analysis of corresponding bands was performed by Image Studio software (LI‐COR Biosciences) ...
• Malaria parasite centrins assemble by Ca²⁺-inducible condensation

  Yannik Voß, et al., bioRxiv - Cell Biology 2023

  Quote: To determine if ECCAs are amyloid aggregates live seeded 3D7-DiCre_pFIO+_Cen1-GFP were stained with 1:1000 Hoechst 3342 and 1:3000 Proteostat (Enzo Life Sciences) in cRPMI for 30 min at 37°C ...
• Chemobiosis reveals tardigrade tun formation is dependent on reversible cysteine oxidation

  Amanda L. Smythers, et al., bioRxiv - Biochemistry 2023

  Quote: Aliquots of the superoxide-reactive spin trap 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine (CMH, 1 mM final concentration) (Enzo Life Sciences, Farmingdale, NY) were prepared in modified Krebs-HEPES buffer containing 99.01 mM NaCl ...
• KvS regulatory subunits confer drug resistance to Kv2 channels

  Robert G. Stewart, et al., bioRxiv - Neuroscience 2024

  Quote: ... Kv2.1 expression was induced in Kv2.1-CHO cells with 1 μg/ml minocycline (cat. #ALX-380-109-M050; Enzo Life Sciences), prepared in 70% ethanol at 2 mg/ml ...
• Modification of an atypical clathrin-independent AP-2 adaptin complex of Plasmodium falciparum reduces susceptibility to artemisinin

  Ryan C. Henrici, et al., bioRxiv - Microbiology 2019

  Quote: ... for 30 min and subsequently incubated with primary antibody for 1 h at room temperature (anti-PDI mouse 1:100 (1D3; Enzo Life Sciences), anti-GFP rabbit 1:200 (A-11122 ...
• The 20S proteasome activator PA28γ controls the compaction of chromatin

  Didier Fesquet, et al., bioRxiv - Cell Biology 2020

  Quote: The following antibodies were used at 1:1000 dilution for immunoblotting and 1-3 μg/ml for immunoprecipitation: anti-PA28γ (rabbit polyclonal BML-PW8190, ENZO Life Sciences), anti-α4 (1:2000 ...
• Epigenetic reprogramming shapes the cellular landscape of schwannoma

  S. John Liu, et al., bioRxiv - Cancer Biology 2022

  Quote: ... All tissues were washed with PBS/EDTA and re-suspended 1:1 with PBS/EDTA and 100 mM Cisplatin (Enzo Life Sciences) for 60 seconds before quenching 1:1 with PBS/EDTA/BSA to determine viability29 ...
• Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancer

  Natalia Vydra, et al., bioRxiv - Cancer Biology 2021

  Quote: ... HSP90 (1:2,000, ADI-SPA-836, Enzo Life Sciences), anti-HSP70 (1:2,000 ...
• Actin remodelling controls proteasome homeostasis upon stress

  Thomas Williams, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-20S (Enzo life sciences; Rabbit; 1:2000; PW9355), anti-Mpk1 (Santa Cruz ...
• Actin remodelling controls proteasome homeostasis upon stress

  Thomas Williams, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-Rpt5 (Enzo life sciences; Rabbit; 1:5000; PW8245), anti-20S (Enzo life sciences ...
• A Drosophila model of neuronal ceroid lipofuscinosis CLN4 reveals a hypermorphic gain of function mechanism

  Elliot Imler, et al., bioRxiv - Neuroscience 2019

  Quote: ... 1:2000 (Enzo Life Sciences Cat# BML-PW8810, RRID:AB_10541840); mouse anti-β-tubulin ...
• A Drosophila model of neuronal ceroid lipofuscinosis CLN4 reveals a hypermorphic gain of function mechanism

  Elliot Imler, et al., bioRxiv - Neuroscience 2019

  Quote: ... 1:2000 (Enzo Life Sciences Cat# BML-PW8810, RRID:AB_10541840); goat anti-HRP Alexa Fluor 647-conjugated ...
• A Drosophila model of neuronal ceroid lipofuscinosis CLN4 reveals a hypermorphic gain of function mechanism

  Elliot Imler, et al., bioRxiv - Neuroscience 2019

  Quote: ... 1:2,000 (Enzo Life Sciences Cat# VAP-SV003E, RRID:AB_2095057); mouse anti-dCSP ...
• Non-canonical localization of RubisCO under high light conditions in the toxic cyanobacterium Microcystis aeruginosa PCC7806

  Tino Barchewitz, et al., bioRxiv - Microbiology 2019

  Quote: ... MC 1:250 (Microcystin-LR, mouse; Enzo Life Sciences); McyB 1:100 (rabbit) ...
• The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity

  Dinh Thi Nguyen, et al., bioRxiv - Cell Biology 2021

  Quote: ... calnexin (SPA-865; Enzo Life Sciences Inc.; 1:1000), KDEL (PM-059 ...
• Molecular and Structural Basis of Olfactory Sensory Neuron Coalescence by Kirrel Receptors

  Jing Wang, et al., bioRxiv - Neuroscience 2021

  Quote: ... rat anti-HSC70/HSP73 1:10,000 (Enzo Life Sciences), or rabbit anti-β-actin 1:1,000 (Cell Signalling Technology) ...
• Branched ubiquitin chain binding and deubiquitination by UCH37 facilitate proteasome clearance of stress-induced inclusions

  Aixin Song, et al., bioRxiv - Biochemistry 2021

  Quote: ... ubiquitin conjugates (FK2; Enzo Life Sciences PW8810, 1:1000); K48-specific ubiquitin (Millipore 05-1307 ...
• Salinomycin disturbs Golgi apparatus function and specifically affects cells in epithelial-to-mesenchymal transition

  Marko Marjanović, et al., bioRxiv - Cancer Biology 2022

  Quote: ... ERGIC53 (1:1000, Enzo Life Sciences, ALX-804-602) and β’COP (1:500 ...
• Systemic muscle wasting and coordinated tumour response drive tumourigenesis

  Holly Newton, et al., bioRxiv - Cancer Biology 2019

  Quote: ... mouse anti-polyubiquitin (FK2; Enzo Life Sciences; 1:300), mouse anti-GRP-78 (E-4 ...
• Antisense oligonucleotides against monoacylglycerol acyltransferase 1 (Mogat1) improve glucose metabolism independently of Mogat1

  Andrew J. Lutkewitte, et al., bioRxiv - Physiology 2020

  Quote: ... Calnexin (Enzo Life Sciences, ADI-SPA-860, 1:1000) and mouse γ-tubulin (Sigma-Aldrich ...
• Differential activation of P-TEFb complexes in the development of cardiomyocyte hypertrophy following activation of distinct GPCRs

  Ryan D. Martin, et al., bioRxiv - Molecular Biology 2020

  Quote: ... or anti-Hsp90 (Enzo Life Sciences, AC88; 1:1000) in 5% milk overnight at 4°C ...
• DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway

  Raffaella Gallo, Arpan Rai, Lucas Pelkmans, bioRxiv - Cell Biology 2020

  Quote: ... Ubiquitin (FK2) (1:200, Enzo Life Sciences, BML-PW8810), mCherry (1:1000 ...
• NFIA regulates granule recruitment and exocytosis in the adult pancreas

  Marissa A. Scavuzzo, et al., bioRxiv - Cell Biology 2019

  Quote: ... 1 μM Forskolin (Enzo Life Sciences BML-CN100-0010), 20 μM PMA (company) ...
• Preferential lipolysis of DGAT1 over DGAT2 generated triacylglycerol in Huh7 hepatocytes

  Rajakumar Selvaraj, et al., bioRxiv - Cell Biology 2021

  Quote: ... calnexin (Cnx) (1:1000, Enzo Life Sciences #SPA-865), glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
• YTHDF2 in dentate gyrus is the m⁶A reader mediating m⁶A modification in hippocampus-dependent learning and memory

  Mengru Zhuang, et al., bioRxiv - Neuroscience 2022

  Quote: ... Bassoon (Enzo Life Sciences, ADI-VAM-PS003, 1:1000), PSD95 (Abcam ...
• The serine/threonine kinase Back seat driver prevents cell fusion to maintain cell identity

  Shuo Yang, Aaron N. Johnson, bioRxiv - Developmental Biology 2022

  Quote: ... α -ERK (1:1000, Enzo Life Sciences, ADI-KAP-MA001), and α -Myc (1:1000 ...
• Frataxin deficiency disrupts mitochondrial respiration and pulmonary endothelial cell function

  Miranda K. Culley, et al., bioRxiv - Cell Biology 2022

  Quote: ... using the Endothelin-1 ELISA kit (Enzo Life Sciences).
• PRMT5-mediated regulatory arginine methylation of RIPK3

  Chanchal Chauhan, et al., bioRxiv - Cell Biology 2022

  Quote: ... Nec-1 (BML-AP309-0020, Enzo Life Sciences, 50µM), PP2 (HY-13805 ...
• Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signalling pathway

  Iqbal Dulloo, et al., bioRxiv - Cell Biology 2022

  Quote: ... HA.11 (Enzo Life Sciences, #ABS120-0200; 1:500), BAP31 (Enzo Life Sciences ...
• Predicting the efficacy of BH3 mimetics through the profiling of multiple protein complexes in acute myeloid leukemia

  Changju Chun, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 2 μM JC-1 (ENZ-52304; Enzo Life Sciences), and 10 mM 2-mercaptoethanol (M3148 ...
• Chronic pharmacologic manipulation of dopamine transmission ameliorates metabolic disturbance in syndrome caused by mutated trappc9

  Yan Li, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti-Bassoon (1:400, Enzo life sciences, SAP7F407) and mouse anti-NeuN (1:500 ...
• The reciprocal regulation between mitochondrial-associated membranes and Notch signaling in skeletal muscle atrophy

  Yurika Ito, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-Laminin 2a (diluted 1/500, Enzo Life Sciences), anti-Dystrophin (diluted 1/200 ...