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• Autophagy contributes to homeostasis in esophageal epithelium where high autophagic vesicle content marks basal cells with limited proliferation and enhanced self-renewal potential

  Alena Klochkova, et al., bioRxiv - Physiology 2023

  Quote: ... and incubated at 37°C for 30 minutes with Cyto-ID (ENZ-51031-K200; Enzo Life Sciences) at 1:1000 dilution ...
• TMEM65 regulates NCLX-dependent mitochondrial calcium efflux

  Joanne F. Garbincius, et al., bioRxiv - Physiology 2023

  Quote: ... 6µM thapsigargin (Enzo Life Sciences # BML-PE180-0005), and 5mM succinate (Sigma-Aldrich # S3674) ...
• Tripartite interactions of PKA catalytic subunit and C-terminal domains of cardiac Ca²⁺ channel modulate its β-adrenergic regulation

  Shimrit Oz, et al., bioRxiv - Biochemistry 2023

  Quote: ... Antibodies used: mouse anti-Hsp90 (Enzo Life Science, #SPA-830); mouse anti-GFP Tag (Thermo Fisher Scientific ...
• Cardiomyocyte mechanical memory is regulated through the talin interactome and DLC1 dependent regulation of RhoA

  Emilie Marhuenda, et al., bioRxiv - Cell Biology 2023

  Quote: ... Y27632 (Enzo Life Sciences, 10 μM), FAK Inhibitor 14 (Cayman Chemical ...
• The protein phosphatase-2A subunit PR130 is linked to cytotoxic protein aggregate formation in mesenchymal pancreatic ductal adenocarcinoma cells

  Alexandra Nguyen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... chloroquine was from Enzo Life Sciences GmbH ...
• Intramembrane protease SPP defines a cholesterol-regulated abundance control of the mevalonate pathway enzyme SQS

  Dönem Avci, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse monoclonal anti-CLIMP63 (G1/296, Enzo Life Sciences, #ENZ-ABS669-0100), mouse monoclonal anti-β actin (AC-15 ...
• Reduced S-acylation of SQSTM1/p62 in Huntington disease is associated with impaired autophagy

  F. Abrar, et al., bioRxiv - Neuroscience 2023

  Quote: ... the protein was immunoprecipitated using the same antibody and detected with rabbit anti-SQSTM1 (Enzo Life Sciences, BML-PW9860). For HA-tagged proteins ...
• Differential developmental blueprints of organ-intrinsic nervous systems

  I-Uen Hsu, et al., bioRxiv - Neuroscience 2023

  Quote: ... mixture that contains 0.5 mg /mL of Matrigel and 33 μg/mL Collagen I (ALX-522-440-0050, Enzo Life Sciences). Cells were then incubated at 37 °C in 5% CO2 and co-cultured for 4-5 days.
• Remodeling of the protein ubiquitylation landscape in the aging vertebrate brain

  Antonio Marino, et al., bioRxiv - Systems Biology 2023

  Quote: ... membranes were incubated overnight with primary antibodies against total ubiquitin-conjugates (Enzo Life Sciences, BML-PW8810), SQSTM1 (Abcam ...
• Pyroptosis leads to loss of centrosomal integrity in macrophages

  Siyi Bai, et al., bioRxiv - Immunology 2023

  Quote: ... E-64-D (BML-PI107) was sourced from Enzo Life Sciences. L-Leucyl-L-Leucine methyl ester (LLOMe ...
• Ancient genomic linkage couples metabolism with erythroid development

  Alexandra E. Preston, et al., bioRxiv - Cell Biology 2023

  Quote: ... Chloroquine was added to one of the wells for each sample (50μM; Enzo Life Sciences; cat. no., ENZ-51031). The plate was incubated at 37 °C and 5% CO2 for 2 h ...
• MFSD1 in complex with its accessory subunit GLMP functions as a general dipeptide uniporter in lysosomes

  Katharina Esther Julia Jungnickel, et al., bioRxiv - Biochemistry 2023

  Quote: ... KDEL (mouse monoclonal, Enzo Life Sciences), Cox IV (rabbit polyclonal ...
• Vertical transmission of maternal DNA through extracellular vesicles associates with altered embryo bioenergetics during the periconception period

  David Bolumar, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-calnexin (1:1,000; ADI-SPA-865; Enzo Life Sciences, Farmingdale, NY, USA), mouse anti-calreticulin (1:1,000 ...
• An mTOR-independent Macroautophagy Activator Ameliorates Tauopathy and Prionopathy Neurodegeneration Phenotypes

  Leonard Yoon, et al., bioRxiv - Cell Biology 2023

  Quote: ... at 1:100 dilution, Amyloid β A4 (CT, 1-42) (8G7) (Enzo Life Sciences) at 1:200 dilution ...
• Clonal stochasticity in early NK cell response to mouse cytomegalovirus is generated by mature subsets of varying proliferative ability

  Darren Wethington, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were stained for viability by mixing 1:1 with PBS/EDTA + 100 mM cisplatin (Enzo Life Sciences) for 1 minute before quenching with PBS/EDTA + 0.5% BSA (Staining Media) ...
• CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnover

  Amera L. Martinez, et al., bioRxiv - Cell Biology 2023

  Quote: ... or 5 µg/ml Control:Fc (Enzo Life Science; ALX-203-004-C050) overnight at 4C ...
• Ancient genomic linkage couples metabolism with erythroid development

  Alexandra E. Preston, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were then processed using the CYTO-ID Autophagy Detection Kit (Enzo Life Sciences; cat. no., ENZ-51031), as per the manufacturer’s instructions ...
• Synapsin E-domain is essential for α-synuclein function

  Alexandra Stavsky, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cumulative exocytosis was achieved by blocking SV reacidification by adding 1 μM bafilomycin A1 (Enzo Life Sciences, Cat#BML-CM110-0100) to the bathing medium itemized above ...
• Decoding transcriptomic signatures of Cysteine String Protein alpha-mediated synapse maintenance

  Na Wang, et al., bioRxiv - Neuroscience 2023

  Quote: ... Primary antibodies used for this study are: Bassoon (Enzo Life Sciences Cat# ADI-VAM-PS003, RRID: AB_10618753), Homer 1 (Synaptic Systems Cat# 160003 ...
• Delineation of the pathogenic presynaptic mechanisms of synaptotagmin-1 variants

  Holly Melland, et al., bioRxiv - Neuroscience 2023

  Quote: ... 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), DL- 2-Amino-5-phosphonopentanoic acid (AP5), and Bafilomycin A1 were distributed by Sapphire Bioscience (Sydney, Australia) from Enzo Life Sciences (Lausen, Switzerland), Cayman Chemical (Ann Arbor ...
• Sleep Disruption in a Mouse Model of Chronic Traumatic Brain Injury

  Andrew R. Morris, et al., bioRxiv - Neuroscience 2023

  Quote: ... and alpha fodrin/alpha-II spectrin (cat # BML-FG6090-0500, Enzo Life Sciences). Protein bands were visualized using horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Abcam ...
• Inducible degradation-coupled phosphoproteomics identifies PP2A^Rts1 as a novel eisosome regulator

  Andrew G. DeMarco, et al., bioRxiv - Molecular Biology 2023

  Quote: ... phytosphingosine (Enzo Life Sciences) and canavanine (Cayman Chemical) ...
• Parkinson’s disease-related Miro1 mutation induces mitochondrial dysfunction and loss of dopaminergic neurons in vitro and in vivo

  Axel Chemla, et al., bioRxiv - Neuroscience 2023

  Quote: ... and 0.75 µM purmorphamine (PMA, Enzo Life Science, cat. no ALX-420-045) until reaching 80% confluency ...
• Effects of transient, persistent, and resurgent sodium currents on excitability and spike regularity in vestibular ganglion neurons

  Selina Baeza-Loya, Ruth Anne Eatock, bioRxiv - Neuroscience 2023

  Quote: ... we thawed stock solutions of pharmacological agents: NaV channel blockers tetrodotoxin (TTX; Enzo Life Sciences, Farmingdale ...
• Pulmonary Matrix Derived Hydrogels from Patients with Idiopathic Pulmonary Fibrosis Induce a Proinflammatory State in Lung Fibroblasts In Vitro

  JG Fernandez Davila, et al., bioRxiv - Molecular Biology 2023

  Quote: ... CCK-8 (Enzo Life Sciences) was added (10 uL/well ...
• Engineering a plant polyketide synthase for the biosynthesis of methylated flavonoids

  Bo Peng, et al., bioRxiv - Biochemistry 2023

  Quote: ... Fatty acid synthase (FAS) inhibitor cerulenin was purchased from Enzo life sciences (USA).
• Calcium transients trigger switch-like discharge of prostaglandin E₂ in an extracellular signal-regulated kinase-dependent manner

  Tetsuya Watabe, et al., bioRxiv - Cell Biology 2023

  Quote: ... BAPTA-AM was obtained from Enzo Life Sciences. PD0325901 ...
• Suppressor of Cytokine Signaling 3 Derived Peptide as a Therapeutic for Inflammatory, and Oxidative Stress Induced Damage to the Retina

  Chulbul M Ahmed, et al., bioRxiv - Immunology 2023

  Quote: ... Supernatants were harvested and used in triplicate to measure the concentration of NO using Greiss reagent (Alexis Biochemicals, Plymouth Meeting, PA), following the manufacturer’s protocol ...
• KSHV Terminal Repeat Regulates Inducible Lytic Gene Promoters

  Yoshihiro Izumiya, et al., bioRxiv - Microbiology 2023

  Quote: ... Hygromycin B solution was purchased from Enzo Life Science (Farmingdale, NY). The following antibodies were used for CUT & RUN ...
• An organic extract from ascidian Ciona robusta induces cytotoxic autophagy in human malignant cell lines

  Alessandra Gallo, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: Autophagy was monitored in live cells using the Cyto-ID Autophagy Detection kit (Enzo Life Sciences, Milan, Italy) (Russo et al. ...
• Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutation

  Yeranuhi Hovhannisyan, et al., bioRxiv - Pathology 2023

  Quote: ... cultured for 3 days at which 1 µg/ml ouabain (Enzo Life Sciences) was added for clonal selection during 3 more days ...
• Predicting the efficacy of BH3 mimetics through the profiling of multiple protein complexes in acute myeloid leukemia

  Changju Chun, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 2 μM JC-1 (ENZ-52304; Enzo Life Sciences), and 10 mM 2-mercaptoethanol (M3148 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... Escherichia coli LPS (Serotype O555:B5, TLRgrade, Cat# ALX-581-013-L002) was purchased from Enzo Life Sciences. Pam3CSK4 (Cat# tlrl-pms) ...
• Multi-parameter phenotyping of platelets and characterisation of the effects of agonists using machine learning

  Ami Vadgama, et al., bioRxiv - Immunology 2023

  Quote: ... 0.3-30μM U46619 (Enzo Life Sciences); 3-100μM protease-activated receptor 4 agonist (PAR-4 ...
• Host E3 ubiquitin ligase ITCH mediates Toxoplasma gondii effector GRA35-triggered NLRP1 inflammasome activation and cell-autonomous immunity

  Yifan Wang, et al., bioRxiv - Immunology 2023

  Quote: ... and Mouse monoclonal anti-Ubiquitin antibodies used for immunofluorescence assay were purchased from Enzo Life Sciences (Cat# ENZ-ABS840-0100). Rabbit polyclonal anti-RNF213 antibodies were purchased from Sigma-Aldrich (Cat# HPA003347) ...
• Incorporation of 5 methylcytidine alleviates innate immune response to self-amplifying RNA vaccine

  Mai Komori, et al., bioRxiv - Immunology 2023

  Quote: ... a mouse anti-RIG-I antibody (Enzo Life Sciences) or a rabbit anti-MDA5 antibody (Proteintech) ...
• MARCO in alveolar macrophages negatively regulates Ace expression and aldosterone production

  Conan J O O’Brien, et al., bioRxiv - Immunology 2023

  Quote: We measured plasma aldosterone and corticosterone using reverse ELISA kits (Enzo Life Sciences) according to manufacturer’s instructions ...
• Social Determinants modulate NK cell activity via obesity, LDL, and DUSP1 signaling

  Yvonne Baumer, et al., bioRxiv - Immunology 2023

  Quote: ... Chloroquine (Enzo Life Sciences) was used at 1μM.
• Influenza A virus activates the unfolded protein response and induces the accumulation of insoluble protein aggregates that are essential for efficient viral propagation

  Mariana Marques, et al., bioRxiv - Microbiology 2023

  Quote: ... staining with Proteostat Aggresome Dectetion kit (Enzo Life Sciences International) was performed for 30 min ...
• Suppression of progesterone by influenza A virus mediates adverse maternal and fetal outcomes in mice

  Patrick S. Creisher, et al., bioRxiv - Microbiology 2023

  Quote: ... P4 was quantified by ELISA according to the manufacturer’s instructions (Enzo Life Sciences ADI-900-11). PGF2α (Alpco 74-PGFHU-E01 ...
• Hormonal plasticity to food restriction is heritable

  Jenny Q Ouyang, Ádám Z Lendvai, bioRxiv - Evolutionary Biology 2023

  Quote: ... we used enzyme-linked immunosorbent assay kits (Enzo Life Sciences; Farmingdale, NY, USA) following the manufacturer’s instructions ...
• ARID1A Mutations Protect Follicular Lymphoma from FAS-dependent Immune Surveillance by Reducing RUNX3/ETS1-Driven FAS-Expression

  Martina Antoniolli, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or 300 ng/mL purified soluble human recombinant FAS ligand (SUPERFASLIGAND®, Enzo Life Sciences, Inc., Farmingdale, New York, USA) for 24 h ...
• Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

  Julien Scaviner, et al., bioRxiv - Cancer Biology 2023

  Quote: ... activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France). Briefly ...
• ‘Off-pore’ nucleoporins relocalize heterochromatic breaks through phase separation

  Chiara Merigliano, et al., bioRxiv - Cell Biology 2023

  Quote: ... and selecting in the presence of 100 µg/ml Hygromycin B (Enzo Life Sciences) or 2 mg/ml Puromycin (Sigma-Aldrich) ...
• CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnover

  Amera L. Martinez, et al., bioRxiv - Cell Biology 2023

  Quote: ... or 5 µg/ml Fc-ICAM-1 (Enzo Life Science; ALX-203-004-C050). NK cells were fixed and permeabilized with CytoFix/CytoPerm (BD Biosciences ...
• No evidence for the presence and control of tumor growth by cytoplasmic stress granules in pancreatic cancer

  Maxime Libert, et al., bioRxiv - Cancer Biology 2023

  Quote: Measure of 15-d-PGJ2 levels was conducted using an ELISA kit (ADI-900-023, Enzo Life Sciences) following the manufacturer’s instructions ...
• Beneficial effects of intermittent fasting in NASH and subsequent HCC development are executed by concerted PPARα and PCK1 action in hepatocytes

  Suchira Gallage, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Corticosterone was measured from flash frozen serum with an ELISA kit (Enzo Life Sciences) according to the manufacturer’s instructions ...
• Pyruvate dehydrogenase kinase expression profile is a biomarker for cancer sensitivity to dichloroacetate-mediated growth inhibition

  Bevan P Gang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 2004) were used to detect proteins of interest with the following primary antibodies: PDK1 (Cat# ADI-KAP-PK112, Enzo Life Sciences, Plymouth Meeting, PA, USA), PDK2 (Cat# AP7039b ...
• Jasmonic acid and methyl jasmonate attenuate neuroinflammation via crosstalk with the prostaglandin E₂/receptor EP2 signaling axis

  Emily L Ward, Philip E Chen, Alaa Hussien-Ali, bioRxiv - Cell Biology 2023

  Quote: ... an enzyme-linked immunosorbent assay kit (UG_ADI-900-066) against intracellular cyclic-AMP was utilized from Enzo Life Sciences (St. Louis, MO, USA).
• Jasmonic acid and methyl jasmonate attenuate neuroinflammation via crosstalk with the prostaglandin E₂/receptor EP2 signaling axis

  Emily L Ward, Philip E Chen, Alaa Hussien-Ali, bioRxiv - Cell Biology 2023

  Quote: ... A total of N=4 experiments were performed and strips of the 96-well microtiter plate (Enzo Life Sciences) were read using a microplate reader at 405nm (BioTek PowerWave 340).