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Citations for Bio-Rad :
601 - 650 of 6179 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... or ∼50 ng (8-14 months post-hatch) of total RNA as template in one-step reverse transcription droplet digital PCR (RT-ddPCR) [66] (BioRad) with TaqMan probes specific to vgll3 alleles to quantify vgll3 absolute expression (Supplementary table 1) ...
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bioRxiv - Bioengineering 2020Quote: A reverse transcription polymerase chain reaction (RT-PCR) was carried out on the RNA using the mix iScript Reverse Transcription Supermix (Biorad) and the following thermocycler program ...
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bioRxiv - Neuroscience 2022Quote: ... was used for the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) by CFX96 Touch qPCR Detection System (Bio-Rad, 1855196). Data was analysed by CFX Manager software (Bio-Rad).
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bioRxiv - Developmental Biology 2020Quote: ... GEM-RT-PCR was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; CT022510) to produce barcode cDNA using the following program ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted as described above, followed by RT-PCR using the Allplex SARS-CoV-2 assay (SeeGene, South-Korea) on a CFX96 thermocycler (Biorad), simultaneously detecting four different target genes ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... PCR plates were sealed using optically clear films and transferred to a C1000 thermal cycler with CFX-96 RT-PCR head (BioRad). The fluorescence intensity was measured over a temperature gradient from 25 to 95 °C at a constant rate of 0.05 °C/s ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed using i-Taq™ DNA Polymerase (Intron) and a T100™ Thermal Cycler system (Bio-Rad). Real-time quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... on cDNA samples using the LightCycler 480 SYBR Green I Master kit in the CFX384 RT-PCR Detection System (Biorad). The identities of the resulting amplicons were checked by melting-curve analysis using the CFX384 software ...
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bioRxiv - Microbiology 2023Quote: ... PCR plates were then sealed and RT-LAMP was carried out using a CFX96 Touch Real-Time PCR Detection System thermocycler (BioRad) following a thermal program ...
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bioRxiv - Microbiology 2023Quote: ... PCR plates were then sealed and RT-LAMP was carried out using a CFX96 Touch Real-Time PCR Detection System thermocycler (BioRad) following a thermal program of continuous 65°C with fluorescence read every 30 s for 180 cycles ...
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bioRxiv - Microbiology 2023Quote: Quantitative RT-qPCR was routinely performed on 0.5 µl cDNA in a CFX96 real-time PCR cycling machine (BioRad, USA) using H ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... A quantitative real-time PCR (RT-qPCR) assay was performed on a LightCycler 480 II system using 2x SYBR Green qPCR master mix (BioRad). Maize GAPDH (Zm00001eb233140 ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed on RNA to produce cDNA for RT-PCR using an iScript Reverse Transcription Supermix (Bio-Rad) using a Bio-Rad T100 Thermal Cycler ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative mRNA expression was determined by real-time RT-PCR using iTaq Universal SYBR Green Supermix (Bio-Rad, 172-5124). Primers used were ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1µg of RNA in 20µl reaction volume was reverse transcribed using the iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad). RT-qPCR was performed for TNFA ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using the BioRad SsoAdvanced Universal SYBR green Supermix according to the manufacterer’s protocol (BioRad #172571). Briefly ...
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bioRxiv - Genetics 2024Quote: Relative quantification of gene expression was analysed by RT-qPCR using CFX96 Touch Real-Time PCR Detection System (Bio-Rad). For the amplification of gene fragments with a length of 150 to 250 bp ...
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bioRxiv - Microbiology 2024Quote: ... The RT-qPCR assay was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules CA) under the following conditions ...
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bioRxiv - Biochemistry 2023Quote: ... The mRNAs levels were measured by RT-PCR (25 µL containing 4 µL of cDNA) on a CFx94 (Bio-Rad) using the Syber Green kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... was used with the primers listed in Supplementary Information Table 2 and samples were run on CFX96 Real-Time PCR Detection System (Bio-rad).
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bioRxiv - Microbiology 2019Quote: ... was determined for different genes (primers are listed in Table 2) by quantitative PCR (qPCR) using iTaq SYBR Green (Bio-Rad) with 400 pg of cDNA and 0.25 μM each primer in 10 μL reactions ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs were performed using 2ul of 1:100 diluted first PCRs as a template with qPCR primers (Table S4) and iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA samples were diluted 1:10 and real-time PCR was performed using IQ SYBR Green supermix and gene-specific primers in the iCycler real-time PCR detection system (Bio-Rad). The Hs-FAP-α primer was designed by using Primer3Plus ...
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bioRxiv - Cancer Biology 2021Quote: ... and indicated primers (2 μL) were added in the PCR reactions (ddPCR Supermix for Probes (No dUTP) mix from Bio-Rad) in a total of 25 μL ...
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bioRxiv - Cell Biology 2021Quote: 500 picogram of eluted DNA was used as a template for qPCR analysis in a 20µL reaction with the following primers on a BioRad CFX96 Touch Real-Time PCR Detection System (BioRad, cat. #1855196) using Power SYBR Green PCR master mix (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with genespecific primers using SsoAdvanced Universal Sybr green Supermix (1725271, Bio-Rad) according to the manufacturer’s protocol (Supplementary Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was carried out with the gene-specific mouse primers using SsoAdvanced SYBR Green supermix (Bio-Rad, Hercules, CA, USA) and CFX96 thermal cycler (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the cDNA library was amplified in the presence of specific primers and 2x SYBR Green qPCR Master Mix (bimake) with a CFX96 real-time PCR detection system (Bio-Rad). The relative expression level of specific genes was analyzed relative to the mean critical threshold (CT ...
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bioRxiv - Immunology 2022Quote: ... and the primers for murine IFNAR 1 and beta-Actin in a MyiQ™ Single-Color Real-Time PCR System (BioRad). Products of RT-PCR were separated by electrophoresis on a 2.5% agarose gel in 1x TBE buffer ...
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bioRxiv - Microbiology 2022Quote: ... with target specific primers and the assay ran on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). A 139-bp fragment was amplified for FBN11228 (F-CCAGCATCGGTACAACGGAA and R-AAGCTCGTGTTTTCGTGCTG) ...
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bioRxiv - Neuroscience 2021Quote: ... diluted cDNA or genomic DNA was subjected to quantitative real-time PCR in triplicate with the indicated primers using Ssofast EvaGreen Supermix (Bio-Rad) in a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCRs were performed in 96-well plates using the PrecisionPLUS qPCR Mater Mix (Primer Design) and the CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The Affymetrix Human Transcriptome Array 2.0 (HTA2 ...
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bioRxiv - Cell Biology 2019Quote: ... with 10 ng of cDNA with specific primers of interest on a CFX Connect real-time PCR Detection System (Bio-Rad). Relative amounts of mRNA were calculated by comparative CT analysis with 18S ribosomal RNA used as internal control ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with gene-specific primers using SsoAdvanced Universal Sybr green Supermix (1725271, Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... supplemented with gene-specific primer pairs (Supplementary Table S10) on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The ACTB transcript was used for the normalization of the target gene.
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using 10-20 ng cDNA as template and gene specific primer pairs shown in Table S8 in CFX Connect Real-Time PCR System (Bio-Rad). The PCR conditions were 95◻°C for 15◻min ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral copy numbers were determined using SARS-CoV-2 RdRp gene specific primers and probe (see [28]) and droplet digital PCR (Bio-Rad Laboratories Inc. ...
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bioRxiv - Genetics 2022Quote: ... a master mix containing primers but not the template was made and aliquoted to 96-well PCR plates (Bio-Rad, HSP9601). Right before loading ...
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bioRxiv - Genetics 2023Quote: ... After 24h genomic DNA was analyzed through gene amplification using a forward and a mutation specific reverse primer by standard PCR (Bio-Rad) using Firepol Taq Polymerase [BioDyne] ...
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bioRxiv - Plant Biology 2024Quote: ... and 500nmol of each primer and annealing temperature of 59 °C on a CFX Connect Real-Time PCR Detection System (Bio-Rad) at 95 °C for two minutes followed by forty cycles of 95 °C for 15 seconds and 59 °C for 30 seconds ...
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bioRxiv - Bioengineering 2024Quote: ... by quantitative real-time PCR using primers that amplify the bGH PolyA sequence (Table S3) and the SsoFast Evagreen mix (Bio-Rad). The virus was stored at -80°C.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was quantified by qPCR with a QuantStudio3 Real-Time PCR machine and transcript-specific primers and iTaq UniverSYBR Green Supermix (Bio-Rad). RNA was normalized to 18S rRNA levels and corrected for primer efficiency.
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bioRxiv - Immunology 2024Quote: ... quantitative real-time polymerase chain reaction (qRT-PCR) using gene-specific primers and universal SYBR Green Supermix (#1725274, Bio-Rad Laboratories) was performed ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was twice washed with 2X SSC (0.15 M NaCl and 0.015 M sodium citrate) and 0.5 % SDS (Bio-Rad) for 30 minutes at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... For the reverse transcriptase (RT) reaction,SYBR Green RT reagents (Bio-Rad, USA) were used ...
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bioRxiv - Molecular Biology 2020Quote: ... Validated PrimePCR primers (Bio-Rad) (Ccl2 qMmuCED0048300 ...
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bioRxiv - Bioengineering 2021Quote: ... PrimePCR™ Primers (Bio-Rad) used were as listed in Table 1 ...