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Citations for Bio-Rad :
51 - 100 of 818 citations for cleaved Caspase 9 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The purified mAbs were buffer-exchanged into PBS using a desalting column (Bio-Rad). mAbs were quantified using their theoretical molar extinction coefficients that are calculated based on the contents of aromatic residues and with absorbance at 280 nM measured using a Nanodrop machine.
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Ab (mAb)-anti-sialoadhesin (Bio-rad, Inc., Hercules, CA, USA; 1:250), goat pAb anti-integrin-α3 (Santa Cruz biotechnology ...
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bioRxiv - Biophysics 2023Quote: Apoptosis was probed using the Bio-Rad Magic Red Caspase 3-7 kit (Bio-Rad, ICT 935). HEK 293T cells stably expressing FGFR1-YFP were seeded in 96 well plates and allowed to grow to ∼70% confluency ...
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bioRxiv - Physiology 2021Quote: ... 9% or 12% -SDS polyacrylamide gel electrophoresis and transferred to PVDF Immobilon membranes (Biorad). After 1h-saturation in TBS/0.2% Tween/2% milk (RT) ...
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bioRxiv - Neuroscience 2022Quote: Samples were loaded on a home-made 9 or 10% Bis-Acrylamide (Bio-Rad) gel and the gel was subsequently transferred by wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... HRP signal was detected in 9:1 mix of Clarity:Clarity Max ECl reagent (BioRad) and captured using a Chemidoc Touch imager (BioRad).
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bioRxiv - Cancer Biology 2021Quote: ... Acid-treated fiber spreads were incubated with 1/1000 rat anti-BrdU mAb (Bio-Rad) for 1.5 hour to detect CldU ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with: IFN-γ-AF647 mAb (clone CC302, Bio-Rad Antibodies, Kidlington, UK), TNF-α-BV421 mAb (clone Mab11 ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with: IFN-γ-Alexa Fluor 647 mAb (clone CC302, Bio-Rad Antibodies,) and TNF-α-Brilliant Violet 421 mAb (clone Mab11 ...
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bioRxiv - Immunology 2020Quote: ... Binding of mAb to glycan was determined with A Bio-Plex 200 HTS (Bio-Rad) machine with the Bioplex manager software (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant XRCC4-6xHis was immobilised on a GLC chip (BioRad Laboratories) in the vertical orientation ...
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bioRxiv - Immunology 2019Quote: Lung homogenates and a pre-stained recombinant protein ladder (Bio-Rad) were run in a 12% SDS-polyacrylamide gel and were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
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bioRxiv - Synthetic Biology 2023Quote: ... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
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bioRxiv - Bioengineering 2021Quote: ... The mAb-containing fractions were finally polished with CHT chromatography with type II resin (Bio-Rad). The CHT columns were equilibrated and washed with phosphate running buffer and eluted with running buffer containing NaCl.
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Macrophage accumulation in atherosclerosis was assessed by staining with anti-CD68 mAb (FA-11, Bio-Rad) and MOMA-2 (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.
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bioRxiv - Molecular Biology 2019Quote: ... Either regular (Figure 4 and 5) or stain-free (Figure 8 and 9, Bio-Rad #1610182) 10 % acrylamide gels were used ...
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bioRxiv - Synthetic Biology 2023Quote: ... equipped with an Aminex HPX-87C column (300 × 7.8 mm, 9 μm; Bio-Rad, CA, USA). The column was maintained at 40°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the DSG-crosslinked SATB2 interaction protein complexes were cleaved from Protein A/G beads by boiling in Laemmli Sample Buffer from Bio-Rad without adding reducing reagents (DTT or 2-Mercaptoethanol ...
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bioRxiv - Microbiology 2019Quote: ... were coated with 1 μg/ml recombinant YFV NS1 (Biorad, cat # PIP052A) in 50 mM carbonate buffer (pH ...
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bioRxiv - Neuroscience 2021Quote: ... using known concentrations of recombinant mouse IFN-γ (Bio-Rad, Oxford, UK) and a capturing of anti- IFN-γ mAb ...
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bioRxiv - Immunology 2020Quote: ... These recombinant proteins were incubated with Affi-Prep Polymyxin Media (Bio-Rad) for 4 h at 4 °C to remove endotoxins and endotoxin-bound proteins ...
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bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-Rad using HuCAL technology ...
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bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-rad using the HuCAL technology ...
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bioRxiv - Microbiology 2021Quote: ... PCR conditions followed the general Bio-Rad recommendations.9 Data was analyzed with QuantaSoft_v1.7 software (Bio-Rad) including automatic Poisson correction.9,11
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bioRxiv - Developmental Biology 2019Quote: ... and gene-specific primers (Supplementary Table 9) on a CFX384 Touch Real-Time PCR Detection System (BioRad). TBP (TATA-binding protein ...
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bioRxiv - Systems Biology 2023Quote: ... then cells stained for analysis by flow cytometry 9 or 10 days after dCas9 infection (BioRad ZE5). In some cases ...
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bioRxiv - Immunology 2022Quote: ... Permeabilised cells were immediately labelled by incubation with anti-bovine IFN-γ-APC mAb (CC302; Bio-Rad Antibodies) diluted in BD PermWash solution ...
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
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bioRxiv - Biophysics 2023Quote: ... Cleavage of LD650-labelled GPΔTM was verified by in-gel fluorescence imaging of uncleaved and cleaved protein on a 4-20% polyacrylamide using a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA) followed by Coomassie staining ...
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bioRxiv - Biochemistry 2021Quote: ... The conjugated beads were then packed into a 9 mL glass column (Bio-Rad laboratories, Hercules, CA, USA). The dimeric APPI (15 mL ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Final recombinant protein concentrations were determined using Quick Start Bradford Protein Assays (Bio-Rad) with bovine serum albumin as the reference protein for standard curves ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
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bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of protein samples were separated by 9% SDS-PAGE gel and transferred onto nitrocellulose membranes (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Plant Biology 2021Quote: ... The following components were added to a reaction tube: 9 μL of iQ SYBR Green Supermix solution (Bio-Rad), 1 μL of 5 mM specific primers ...
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bioRxiv - Immunology 2022Quote: We continued from aforementioned prep steps as follows: after addition of mAbs plates were covered with foil Microseal (Bio-Rad), incubated on ice for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
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bioRxiv - Immunology 2023Quote: ... and 5 h later cells were stained with mAbs against CD4-PerCP-Cy5.5 (as in Table S2) and CD8β-FITC (clone PPT23, Bio-Rad). The cells were fixed and permeabilized with BD Cytofix/Cytoperm (BD Biosciences ...
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bioRxiv - Bioengineering 2020Quote: ... EG depletion was monitored using on an Aminex HPX-87H ion exclusion column (300 mm x 7.8 mm, particle size 9 μm; Bio-rad). The column was maintained at 40°C and samples were isocratically eluted using 0.014 N H2SO4 at a flow rate of 0.55 ml min-1 and read on a refractive index detector (RID) ...
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bioRxiv - Immunology 2022Quote: ... Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9-13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 6-week-old rosette leaves and bolting flower buds of Col-0 and one of the RPF2-atp1 (RPF2-atp1-9) transformed lines (T3) with PureZol reagent (BioRad). Three independent libraries for each genotype were made from total RNA treated with 250 ng of Turbo DNase (Ambion ...
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bioRxiv - Synthetic Biology 2022Quote: ... All reagents except the double-stranded beacon were first assembled in a total volume of 9 μL and run at 37 °C in a CFX96 real-time PCR detection system (Biorad). Both WT SpCas9 and SpCas9-NG are highly active at this temperature ...
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bioRxiv - Biochemistry 2022Quote: ... rabbit (BIO-RAD, product number ...
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bioRxiv - Immunology 2023Quote: ... Rabbit complement (BioRad) was added and incubated for 60 min ...