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Citations for Bio-Rad :
1 - 50 of 8735 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Total protein concentration of purified DENV VLP was measured by Quick Start Bradford Protein Assay (Bio-Rad). Purity of the DENV VLP was assessed by SDS-PAGE followed by Coomassie dye-based staining using QC colloidal Coomassie stain (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was detected using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Immunology 2023Quote: VLPs were denatured by heating in 2xLaemmli buffer containing 2-mercaptoethanol (Bio-Rad) for 10 minutes at 95°C ...
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bioRxiv - Immunology 2023Quote: ... VLPs were denatured by heating in 4xLaemmli buffer containing 2-mercaptoethanol (Bio-rad) for 10 minutes at 95°C ...
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bioRxiv - Immunology 2024Quote: VLPs were denatured by heating in 2xLaemmli buffer containing 2-mercaptoethanol (Bio-Rad) for 10 minutes at 95°C ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Biophysics 2020Quote: ... 10μl of VLPs or cell extracts were then denatured by Laemmli sample buffer (BioRad, Hercules, CA) with 5% BME and boiled at 95C for 10min ...
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bioRxiv - Biochemistry 2022Quote: ... blots were imaged using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Immunology 2021Quote: ... Protein 1ug/ml were immobilized onto NC membranes (0.22-m pore size; Bio-Rad). The membrane kept for drying for 5-10 min ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred onto a 0.45-m-pore-size polyvinylidene difluoride (PVDF) membrane (Bio-Rad, #1620177) and processed for immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Synthetic Biology 2022Quote: ... Concentration of the VLPs was estimated by Bradford assay and densitometry using SDS PAGE analysis of serially diluted VLP preparations on Mini-PROTEAN Precast Gels (Bio-Rad), stained with GelCode Blue Stain (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the DENV VLP was assessed by SDS-PAGE followed by Coomassie dye-based staining using QC colloidal Coomassie stain (Bio-Rad). The morphologies of DENV VLPs were analyzed at the National Institute of Infectious Disease in Japan (NIID ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Immunology 2019Quote: ... LNs were then left in PBS/0.2% Triton X-100/20% DMSO (Sigma)/0.3 M glycine (BioRad) overnight at RT and blocked in PBS/0.2% Triton X-100/10% DMSO/6% donkey serum (Jackson Immunoresearch ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Labeling efficiency was determined by measuring the absorbance of pyrene (χ346nm = 42,000 M-1cm-1) and protein concentration by 660 nm assay (Bio-Rad). An SDS-PAGE shift assay for isolating single-mSA bound ...
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bioRxiv - Biophysics 2024Quote: ... supernatant was filtered with 0.45 µm filter (Fisher) and loaded to HiTrap Q column (Cytiva) on an NGC Fast Protein Liquid Chromatography (FPLC) station (Bio-Rad). The flow-through fraction was mixed with CM Sepharose FF resin (GE HealthCare Lifesciences ...
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Microbiology 2023Quote: ... extracellular proteins were separated on SDS 15% (m/v) polyacrylamide gels and electroblotted to Immun-Blot polyvinylidene difluoride membranes (Bio-Rad, USA) using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Cell Biology 2023Quote: ... Total nascent peptides were detected by in-gel imaging at 647 µm and total protein was detected by far red epi imaging after staining with 0.1% Coomassie brilliant blue (BioRad ChemiDoc MP Imaging System). For detection of nascent insulin and tubulin ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were transferred to C. sporogenes by conjugation as described before (Heap et al., 2009) using E. coli CA434 (E. coli HB101 (Bio-Rad Laboratories, The Netherlands) harboring the broad host IncPß+ conjugational plasmid pRK24 (Williams et al. ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was twice washed with 2X SSC (0.15 M NaCl and 0.015 M sodium citrate) and 0.5 % SDS (Bio-Rad) for 30 minutes at 37 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... diluted 1:200 in 10 mM Tris-HCl pH 7.5 and 9 μL loaded (along with 1 μL 1 M DTT and 10 μL 2X Laemmli Buffer) into an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-rad). For Western blot ...
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bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2019Quote: ... The gel was then soaked in three times its volume of denaturation solution (0.5 M NaOH, 1.5 M NaCl) for 30 minutes and then transferred to Zetaprobe (Bio-Rad) membrane by means of a vacuum at 55 mBar for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
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bioRxiv - Plant Biology 2021Quote: ... plants were irradiated with UV-B lamps using fixtures mounted 30 cm above the plants (2 W m−2 UV-B and 0.6 W m−2 UV-A, Bio-Rad ChemiDoc™XRS UV-B lamps ...
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bioRxiv - Bioengineering 2021Quote: ... and 0.3 M glycine (Bio-Rad, 161-0718) in 1X PBS ...
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bioRxiv - Plant Biology 2021Quote: ... 60 mg Tungsten M-10 microcarrier (Bio-RAD) was placed into a 1.5 ml Eppendorf tube with 1 mL 70% ethanol ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 M 2-Mercaptoethanol (Bio-Rad), and boiled for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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bioRxiv - Cancer Biology 2019Quote: ... 2 M thiourea and 65 mM dithiothreitol (BIORAD, Australia). Isoelectric focusing (IEF ...
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bioRxiv - Neuroscience 2022Quote: ... and beta mercaptoethanol (0.05 M, BioRad Laboratories Cat#1610710) and loaded into a NuPAGE 4%–12% or 10% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The blood was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad) before use in the mosquitoes feeding process ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Fast Virus 1-step Master Mix) using CFX-96 (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2023Quote: Samples were loaded onto e-PAGEL gel (ATTO) or TGX FastCast gel (Bio-Rad), electrophoresed ...
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bioRxiv - Microbiology 2021Quote: Total viral RNA was extracted from 0.1 ml virus stocks using Aurum Total RNA Mini Kit (Bio-Rad) and eluted in 50 μl elution buffer pre-heated to 70°C ...
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bioRxiv - Microbiology 2021Quote: ... All serum samples were confirmed as Dengue virus-positive by means of NS1 diagnostic ELISA test (Platelia, Biorad). Serum samples were filtered using Millipore 0.22 μm PES syringe filters ...
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bioRxiv - Neuroscience 2024Quote: ... then transferred to PVDF 0.22 m membrane (Bio-Rad, Hercules, CA). The membrane was blocked for 1 hour with 5% bovine serum albumin (BSA ...
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bioRxiv - Genomics 2022Quote: ... denatured proteins and Precision Plus Protein™ Dual Xtra Prestained Protein Standards (Biorad 1610377) were loaded into 16.5% Mini-PROTEAN® Tris-Tricine Gels (Biorad 4563066 ...
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bioRxiv - Microbiology 2022Quote: ... Total protein was determined in all samples by Protein Assay (Protein Assay II, BioRad) according to the manufacturer’s instructions ...