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Citations for Bio-Rad :
151 - 200 of 363 citations for Yeast Extracts since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Each extract was assayed for protein levels with the Bio-Rad protein assay (Bio-Rad, USA). Enzyme activity (expressed in nkatal/mg protein ...
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bioRxiv - Biochemistry 2021Quote: ... Nuclear protein extracts were obtained as described earlier(30) and quantified by Bradford reagent (Bio-rad) to determine the total amount of protein ...
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bioRxiv - Physiology 2021Quote: ... Approximately 8μg of protein extract was loaded per lane on polyacrylamide gel (4-20% Criterion, BioRad). Proteins were separated and transferred to PVDF membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were separated by electrophoresis on acrylamide gel (456-8085, Bio-Rad, Hercules, CA, USA) and transferred onto PVDF membrane ...
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bioRxiv - Biophysics 2020Quote: ... 10μl of VLPs or cell extracts were then denatured by Laemmli sample buffer (BioRad, Hercules, CA) with 5% BME and boiled at 95C for 10min ...
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bioRxiv - Developmental Biology 2022Quote: ... Whole cell extracts were fractionated by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). After incubation with 5% milk in TBST (10 mM Tris ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... cell extracts were quantified with the Bradford-based Protein Assay kit (Bio-Rad Protein Assay, #5000006). After addition of 6X Laemmli buffer (375 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Denatured protein extracts were separated by SDS-PAGE on 10% TGX Stain-Free™ (Bio-Rad) gels prepared according to the manufacturer’s instructions using a Stain-Free FastCast Acrylamide Kit ...
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bioRxiv - Cell Biology 2019Quote: ... The protein extracts were subjected to electrophoresis through a 4–20% precast polyacrylamide gel (Bio-Rad). Subsequently ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell extracts were sonicated 10 s and protein concentration determined using DCTM Protein Assay (Bio-Rad). Ten micrograms of proteins were loaded and run on NuPAGE 3-8% Tris-Acetate (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... protein extracts were loaded to fast protein liquid chromatography (FPLC, NGC Quest 10 Chromatography System, Biorad) for 3X PBS buffer exchange and purification ...
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bioRxiv - Genetics 2022Quote: ... Protein extracts and supernatants were resolved by electrophoresis in Criterion TGX 10% precast gels (Bio-Rad), with the resulting bands transferred onto PVDF membranes (#1704157 ...
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bioRxiv - Neuroscience 2023Quote: ... 30μg whole-cell extracts were separated by SDS–PAGE and transferred onto Nitrocellulose membrane (Bio-Rad). After blocking in 5% milk in TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the cell extracts was determined using the BCA assay kit (Bio-Rad). Protein concentration for lysates was adjusted to 2 μg/μl before adding 4X loading buffer (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of extracts were resuspended in reducing (5.0 % 2-mercaptoethanol) sample buffer (Bio-Rad) and boiled at 100°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of the extracts was measured using a commercial reagent (Bio-Rad Protein Assay). Acetyltransferase activity was assessed using the phosphocellulose paper binding assay [Haas & Dowding ...
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bioRxiv - Physiology 2023Quote: ... These extracts were loaded onto a 4-20 % SDS-PAGE gel (Bio-Rad, Hercules, California, USA), transferred onto nitrocellulose membrane and revealed with a rabbit anti-target antibody (see Table E2) ...
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bioRxiv - Plant Biology 2024Quote: Total protein extract (10 µg) was separated by SDS-PAGE and transferred to PVDF membranes (BioRad). For detection of biotinylated proteins as result of TurboID activity ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the extracts were determined using a Bio-Rad protein assay kit (Biorad, 5000006). Proteins were diluted in Invitrogen 2X sample buffer (NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the extracts were determined using a Bio-Rad protein assay kit (Biorad, 5000006). The same amounts of proteins were incubated with antibodies (anti-SURF2 or anti-GAPDH antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the extracts were determined using a Bio-Rad protein assay kit (Biorad, 5000006). The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μg of protein extracts were mixed with 4x Laemmli sample buffer with 2-mercaptoethanol (Biorad) and heated at 95°C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... yeast (24 h post-induction) were counted using the TC20™ automated cell counter (Bio-Rad Hercules, CA, USA). Cells were centrifuged at 4,000 × G for 10 min and resuspended in PBS (Thermofisher) ...
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bioRxiv - Bioengineering 2024Quote: ... Transformation of yeast cells by electroporation was carried out as in [27] with a Gene Pulser electroporator (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration of the supernatant (“total protein extract”) was determined by Bradford protein assay kit (Bio-Rad). An equal amount of total protein was loaded on 12% SDS acrylamide gels (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Protein extracts collected after centrifugation were subjected to SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). After blocking with PBS containing 0.1% Tween-20 and 5% milk ...
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bioRxiv - Developmental Biology 2019Quote: Nuclear extracts were fractionated by SDS-PAGE and transferred to nitrocellulose by semi-dry transfer (Bio-Rad). Membrane were blocked with 5% nonfat milk in PBS-0.1% Tween 20 for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were deposited onto a 4-15% Mini-PROTEAN® TGXTM precast protein gel (Bio-Rad) for migration ...
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bioRxiv - Cancer Biology 2020Quote: Cell extracts were harvested by direct addition of 2x SDS sample buffer (1610747, BioRad Laboratories, Alcobendas, Spain) containing 100 mM DTT to the culture plate and incubation on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: 30 μg protein extracts were separated on 7.5% gradient polyacrylamide gel (Mini-PROTEAN TGX gel, Bio-Rad) by electrophoresis (SDS-PAGE) ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein extract were loaded onto 4-15% Mini-PROTEAN TGX precast gels (Bio-Rad), separated by SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μl of the protein extract was used to carry out a Bradford (Bio-Rad, 500-0205) assay ...
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bioRxiv - Cancer Biology 2019Quote: ... Extracts were separated by SDS– PAGE and transferred to 0.2 µm pore size nitrocellulose membranes (Bio-Rad). Blots were probed with primary antibodies to caspase-3 (#9662) ...
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bioRxiv - Plant Biology 2020Quote: ... The remaining extract was used for total protein determination using the Bradford microplate microassay procedure (Bio-Rad).
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bioRxiv - Genomics 2021Quote: ... whole-cell extract in the supernatant was measured using the Bio-Rad Protein Assay (Bio-Rad, 5000006). 15 μg of protein extracts were mixed with NuPAGE LDS Sample Buffer (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... Total soluble protein content of the protein extracts was determined using Bradford protein assay (Bio-Rad, USA) with bovine serum albumin (BSA ...
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bioRxiv - Genomics 2022Quote: ... 20 µg total protein extract per sample were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cytoplasmic extract prepared from the harvested cells was used for immunoprecipitation (IP) using ProteinG magnetic beads (Biorad). Beads were first equilibrated with IP buffer (25 mM Tris-Cl ...
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bioRxiv - Microbiology 2023Quote: ... 7 µg of each cell extract was loaded on a 12% stain-free SDS-PAGE gel (BioRad) and separated by gel electrophoresis at 175 V for 45 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 µg of protein extract was loaded on 10% polyacrylamide gels (TGX Stain-Free FastCast, Bio-Rad). Membranes were blocked with 5% BSA-TBST (5% BSA Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... The extracts were then desalted on Bio-Gel® P-6DG gel (BIO-RAD, Hercules, CA, USA) equilibrated with 20 mM sodium phosphate buffer pH 7.4 containing 0.15 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pML104-GAL1 and repair DNA were co-transformed into yeast by electroporation under Gene Pulser Xcell (Bio-Rad) conditions ...
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bioRxiv - Molecular Biology 2020Quote: Yeast genomic DNA was prepared in an agarose plug as described in the Instruction Manual (Bio-Rad, CHEF-DR II) with slight modifications ...
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bioRxiv - Genomics 2022Quote: Chromosomes from stationary yeast cell cultures were prepared in agar molds using the Certified Megabase Agarose (Bio-Rad CAT. 1613108) and pulsed-field gel electrophoresis (PFGE ...
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bioRxiv - Cell Biology 2023Quote: ... Yeast morphology was observed and recorded using a Gel Doc XR+ Gel Documentation System (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Biophysics 2023Quote: ... and yeast cell growth was monitored by measuring the OD600 at 2 h increments in a microplate reader (Benchmark Plus, BioRad). The remainder of the 10 ml cultures was used to assess Pgp expression by Western blot analysis of microsomal membrane preparations using the monoclonal C219 anti-Pgp antibody.
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bioRxiv - Developmental Biology 2020Quote: ... Protein extract equivalent to 50 μg was loaded per lane onto precast gradient gels (4-20% Bio-rad). Separated proteins were then transferred to nitrocellulose membranes ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... protein extracts were prepared in lysis buffer with 2% SDS and resolved in a 4-12% gel (Biorad). Membranes were probed overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 15 µg of protein extract were loaded with 5x Laemmli buffer on Mini Protean TGX gels (Bio-Rad) and run at 100V for 1.5 h ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of whole cell extracts and tissue lysates were determined using a Bradford Protein assay (Bio-Rad). 50μg of sample proteins per well were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF ...