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Citations for Bio-Rad :
251 - 300 of 363 citations for Yeast Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were electrophoresed under reducing conditions on 4– 15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The protein extracts were resolved on 4-15% Criterion™ Tris-HCl precast gels (#3450029, Bio-Rad Laboratories, CA, USA) and blotted onto PVDF membranes (#1704157 ...
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bioRxiv - Genetics 2021Quote: ... Proteins (7 μl of extract) were separated on Laemmli SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, 1620112). Membranes were stained with Ponceau-S for visualization ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Neuroscience 2021Quote: ... The whole extracts (20 µg) were loaded on 10% SDS-PAGE gel and blotted on PVDF (Polyvinylidene fluoride) membrane (Biorad) with wet apparatus (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... Denatured protein extracts were separated by SDS-PAGE on 10% TGX Stain-Free™ Fast-Cast™ gels (Bio-Rad). Separated proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Genomics 2019Quote: ... media were collected for insulin determination and rat islet cells were lysed with acid-ethanol (0.2 mM HCl in 75% ethanol) to extract total insulin content or with protein lysis buffer to measure total protein content (Bradford, BioRad). The amount of insulin released in the medium and remaining in the cells was measured by insulin Elisa kit (Mercodia) ...
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bioRxiv - Genomics 2020Quote: 20 μg of protein extracts in Laemmli buffer were run on a 4-20% gradient pre-cast gel (Bio-Rad) and transferred to nitrocellulose using Turbo transfer packs (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... The testicular extract was run on a SDS-PAGE 4-15% mini-protean TGX precast gel (Bio-Rad, 456-1084) and transferred to a nitrocellulose membrane ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins from bacterial extracts were separated by electrophoresis on 15% polyacrylamide gels and transferred onto nitrocellulose membranes using a wet blotting apparatus (BioRad). Membranes were blocked with 5% milk in PBST (Phosphate buffer saline ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were stored at -20°C or immediately resolved on an 7.5% SDS-PAGE pre-cast gel (Bio-Rad, #4568023) and transferred onto a 0.2 μm nitrocellulose membrane (GE healthcare ...
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bioRxiv - Physiology 2021Quote: Equal amounts of skeletal muscle protein extract (45µg) were separated on Mini-Protean TGX precast protein gels (Biorad, CA, USA) and transferred to a PVDF membrane using a Trans-Blot semi-dry transfer system (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrations of the extracts were determined using the Bio-Rad Protein assay kit (Bio-Rad Laboratories Inc. Hercules, CA). The extracts were then aliquoted into individual tubes ...
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bioRxiv - Physiology 2021Quote: ... About 30 μg of protein from the sample extracts were electrophoresed in 12% Mini-PROTEAN TGXTM precast gels (Bio-Rad) using the electrophoresis system mini-PROTEAN Tetra Cell (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Soluble extract was collected and total protein concentration determined using Protein Assay Dye Reagent Concentrate (BIO-RAD, Cat No 5000006) with BSA as standard ...
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bioRxiv - Molecular Biology 2020Quote: ... nuclear or histone extracts were resolved by SDS-PAGE and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (BioRad). Membranes were blocked in 5% milk in 1x PBS with 0.1% Tween 20 (PBST ...
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bioRxiv - Pathology 2022Quote: ... The soluble protein extracts were separated by SDS-PAGE (4-15% mini-protein TGX precast gel, 12 or 15 well, BioRad) and transferred to a PVDF membrane (Trans-blot Turbo mini-Format 0.2 μM PVDF ...
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bioRxiv - Microbiology 2020Quote: One hundred μg of the different PAO1 cytoplasmic extracts were loaded onto conventional SDS-PAGE 10% Bis-Tris gels (mini-protean TGX Stain-free precast Gels, BioRad). The gel was stained with Coomassie blue and each lane was cut into 10 bands ...
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bioRxiv - Biochemistry 2020Quote: Aliquots (250 μg total protein) of hippocampal extracts of WAR animals subjected to freeze/thaw were treated with 5 μL of dithiothreitol (Biorad) 50 μg/μL ...
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bioRxiv - Cell Biology 2019Quote: Cell extracts were run on an SDS-PAGE gel and then transferred to a PVDF membrane (Bio-Rad, Hercules, CA). Membranes were probed with primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed directly in sample buffer and protein extracts were separated on a 4-20% polyacrylamide gel (Bio-Rad). Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53 ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in RIPA buffer and protein extracts from equal numbers of cells were resolved on a 12% SDS-PAGE gel (Biorad), followed by transfer onto Immun-Blot PVDF membranes (Biorad) ...
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bioRxiv - Biochemistry 2022Quote: ... the amount of GFP-bound protein was assessed by loading final extract in sample buffer (without boiling) on a Mini-PROTEAN TGX Precast Gel (BioRad), visualised with LED488 illumination (G-box ...
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bioRxiv - Biochemistry 2022Quote: ... Whole-cell extracts were prepared from cells grown in YPD and analyzed by SDS-PAGE using stain-free precast gels (Biorad) followed by westernblotting with monoclonal anti-GFP antibodies (clones 7.1 & 13.1 ...
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bioRxiv - Physiology 2022Quote: Equal amounts of protein extracts were resolved by SDS–PAGE using 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of crude cell extracts was measured using the Bradford method with BSA as a standard (Bio-Rad). The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem ...
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bioRxiv - Pathology 2023Quote: ... Equal amounts of protein extract (50 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Bio-Rad, CA, USA) and transferred electrophoretically to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants containing the protein extracts were transferred into fresh 1.5 ml tubes and protein concentration was measured by Bio-Rad Protein Assay Kit using BSA as protein standard (5000002 ...
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bioRxiv - Cell Biology 2023Quote: cDNA was prepared after extracting total RNA from young adult worms or cell extracts using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations of the cellular extracts were determined using the DC Protein Assay (ref 5000112, Bio-Rad, Hercules, California, USA). Equal amounts of proteins (20 μg ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations of brain and olfactory extracts were determined using the RC DC Protein Assay kit (Bio-Rad, Hercules, CA) as described (Miller et al. ...
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bioRxiv - Physiology 2024Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated in 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼45 min followed by 110 V for ∼65 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Neuroscience 2023Quote: Proteins in the nuclear extracts (60 μg protein) were separated by SDS–PAGE gels (456-1043, Bio-Rad, Hercules, CA), transferred onto PVDF membranes (162-0174 ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 μg protein extracts were separated by sodium dodecyl sulfate (SDS) gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were mixed with Laemmli sample buffer and separated by SDS-PAGE using 4-20% TGX gels (Bio-Rad) and transferred to 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... the total protein concentration in the nuclear and cytoplasmic extracts was measured using Protein Assay Dye Reagent Concentrate (Biorad, 5000006). 1 mg nuclear or 3 mg cytoplasmic extract per pulldown was incubated in triplicates with 15 μl of a 50% slurry of GFP-trap beads (ChromoTek ...
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bioRxiv - Microbiology 2024Quote: Equal amounts of Total Protein extracts were migrated on a 12.5% SDS-PAGE gel and transferred onto Immun-Blot PVDF blotting membrane (Bio-Rad). Transfer was performed with the Bio-Rad Transfer System (30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The protein concentrations in each cell extract were measured with the Bradford assay (SmartSpec Plus spectrophotometer, Bio-Rad, Hercules, CA) using the Bradford dye reagent and bovine standards (BioRad ...
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bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations of the cellular extracts were determined using the DC Protein Assay (ref 5000112, Bio-Rad, Hercules, California, USA). Equal amounts of proteins (20 μg ...
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bioRxiv - Cell Biology 2024Quote: ... Extracts were then electrophoresed on 12% Tris-acrylamide SDS-PAGE gel and blotted onto a PVDF membrane (#1620177, Bio-Rad). Membranes were blocked with 5% milk solution for 1 hour at room temperature and probed with primary antibody overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: DNA quantification of the mock casework extracts was performed on a CFX96 Touch real-time PCR instrument (Bio-Rad Laboratories). The RB1 qPCR assay [36] was applied with 10X Immobuffer (Meridian Bioscience ...
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bioRxiv - Biophysics 2021Quote: To extract lipids, purified SaPgsA (5.82 mg ml-1, 200 μl) was mixed with 40 mg SM2 Biobeads (Bio-rad, USA) and gently shaking overnight at 4 °C to remove detergent ...
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bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...