Labshake search
Citations for Bio-Rad :
601 - 650 of 1885 citations for Trefoil Factor 3 TFF3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... alongside an HRP-linked goat anti-rabbit secondary antibody (BioRad).
-
bioRxiv - Developmental Biology 2023Quote: ... Signals were developed with HRP-labeled secondary antibodies (Bio-Rad). Blots were developed using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
-
bioRxiv - Bioengineering 2023Quote: ... Conjugated antibodies used were: FITC anti-CD73 (#MCA6068F, Bio-Rad), APC anti-CD90 (#MCA90APC ...
-
bioRxiv - Cell Biology 2022Quote: ... the vWF antibody was replaced by vWF (Bio-Rad, #AHP062T). Cells were washed three times with 1x PBS followed by incubation with the secondary antibody on the shaker for an hour ...
-
bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...
-
bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...
-
bioRxiv - Immunology 2023Quote: ... goat anti-human– horseradish peroxidase (HRP) secondary antibody (Bio-Rad) at a 1:3,000 dilution was applied for 1 h at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... and goat anti-mouse-HRP antibody (Bio-Rad, 170-6516) was used at 1:3,000 ...
-
bioRxiv - Plant Biology 2024Quote: ... Goat anti-mouse Kappa Light Chain antibody (Bio-Rad, #105001G) and Goat Anti-Rabbit (IgG (H + L)-HRP Conjugate #1706515 ...
-
bioRxiv - Bioengineering 2023Quote: ... Conjugated antibodies used were: FITC anti-CD73 (MCA6068F, Bio-Rad), APC anti-CD90 (MCA90APC ...
-
bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated with respective HRP conjugated secondary antibodies (Biorad) for 1 hour at room temperature and imaged by the ChemiDoc Imaging System (Biorad) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Mouse monoclonal anti-Human Vinculin (V284) antibody (Bio-Rad, MCA465GA), 1:1,000 ...
-
bioRxiv - Biochemistry 2023Quote: ... then with HRP- conjugated anti-mouse secondary antibody (BioRAD 1706516) and visualized with chemiluminescence ...
-
bioRxiv - Biochemistry 2023Quote: ... The secondary antibodies (rabbit and mouse) were purchased from Biorad and the HRP signal was developed using Clarity Western ECL Substrate (Biorad).
-
bioRxiv - Cell Biology 2023Quote: ... 1:5000 anti-Mouse HRP antibody (Bio-rad,5178-2504) and 1:5000 anti-Rabbit HRP antibody (Bio-rad,5196-2504) ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed by HRP-conjugated goat anti-rabbit antibodies (Bio-Rad). ECL substrate (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... either Goat anti-mouse HRP-conjugated antibody (Cat# 1705047, Biorad) or Goat anti-rabbit HRP-conjugated antibody (Cat# 1705046 ...
-
bioRxiv - Cell Biology 2023Quote: ... or Goat anti-rabbit HRP-conjugated antibody (Cat# 1705046, Biorad) or IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (926-68070 ...
-
bioRxiv - Microbiology 2024Quote: ... and secondary antibody (anti-rabbit HRP, 1:10000, Bio-Rad) before washed and imaged (GE Amersham Imager 600).
-
bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, 1706515 or 1706516), diluted in TBS-Tween containing 0.5% BSA ...
-
bioRxiv - Cell Biology 2024Quote: ... a rat anti-mouse CD206 primary antibody (Bio-Rad, USA), or a rat anti-mouse F4/80 primary antibody (Abcam ...
-
bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
-
bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... were added to this for a further 4 h before samples were centrifuged at 10,000 x g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... tuberculosis Rv1630 and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) housekeeping gene was performed using 200 ng RNA in a T100 Thermal Cycler (Bio-Rad). The reaction was carried out with Reverse Transcription Reagents (Applied Biosystems ...
-
bioRxiv - Genetics 2021Quote: ... separated using SDS-PAGE (4 to 12 % gradient gel at 150 V for 2.5-3 h) and transferred to a nitrocellulose membrane overnight (∼16 h) at 20 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5 % fat-free milk in 20 mmol/l Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
-
bioRxiv - Physiology 2022Quote: ... membranes were rinsed 3 times with 1X TBST and the blots were incubated in Clarity ECL western blot substrate solution (Bio-Rad) for 5 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The proportion of each splice isoform was quantified using the ImageJ software.
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 2-3 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
-
bioRxiv - Bioengineering 2019Quote: Proliferation of MSCs was evaluated by determining the total cell count per well (n=3 wells/treatment/time-point) using a TC20 Automated Cell Counter (Bio-Rad).
-
bioRxiv - Cell Biology 2019Quote: Home-made gels: The home-made Laemmli resolving gel (10 ml) contains 3 ml of 40% Acrylamide/Bis (cat#: 1610148, Bio-Rad), 2.5 ml of 1.5M Tris-HCl pH 8.8 (cat# ...
-
bioRxiv - Zoology 2019Quote: ... 0.5 ml of the heptane phase were mixed with ethanol in a 1:3 ratio (v/v) and the absorbance at 233 nm was measured on a SmartSpec Plus spectrophotometer (Bio-Rad) with extraction blanks used as references ...
-
bioRxiv - Cell Biology 2019Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Thermal cycler, BIO-RAD), followed by cooling for 2 minutes at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad, Hercules, CA, USA).
-
bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with gl3 specific primers (Supplemental Table 3) and iQ™ SYBR® Green Supermix (BioRad, USA) and conducted on a BioRad CFX with 96-well reaction blocks under the following PCR conditions ...
-
bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
-
bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
-
bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...
-
bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before detection using Clarity Western ECL Substrate (Bio-Rad 170-5061) and imaging on a ChemiDoc MP (Bio-Rad 17001402).
-
bioRxiv - Bioengineering 2022Quote: Samples were washed in PBS and fixed in 3% paraformaldehyde (PFA) for 15 minutes at room temperature (RT) and permeabilized by 0.5% Triton x-100 (Bio-Rad, USA) for 10 minutes ...
-
bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... Specific primers against the genes of interest (Table 3) were designed and qPCR was performed to amplify the genes of interest using the CF96X Real Time System (BioRad, USA). The qPCR data were analyzed using the ΔΔCt method as described previously150 ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 ml of supernatant was diluted with 3 ml of distilled H2O and applied to freshly prepared Dowex columns (AG1-X8; Bio-Rad). Columns were washed twice with distilled H2O ...
-
bioRxiv - Developmental Biology 2022Quote: ... antibody-bead complexes were washed 3 times with lysis buffer before eluting the samples by boiling for 10 minutes in 4x loading dye (Bio-Rad) containing 10% β-mercaptoethanol ...