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Citations for Bio-Rad :
301 - 350 of 701 citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 2-3 μg of total protein were loaded onto 10% SDS-polyacrylamide (29:1 Bio-Rad) gels with 10 μM phostag reagent (FUJIFILM Wako Chemicals ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were resolved on 7 cm pH 3-10 immobilized pH-gradient (IPG) strips (Bio-Rad), under mineral oil ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were washed with TBST 3 times and imaged using the ChemiDoc MP imaging system (BioRad).
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bioRxiv - Molecular Biology 2020Quote: ... Normalised samples (1 – 3 µg) were electrophoresed on 12% Mini-Protean TGX Stain-Free gels (BioRad) or 4-20% Criterion TGX Stain-Free Precast gels (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were washed 3 times and then eluted using 2 × Laemmli Sample Buffer (Bio-Rad). The elution was subjected to SDS PAGE to run the sample into the lane ...
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bioRxiv - Systems Biology 2022Quote: ... Each aliquot was heated to different temperatures for 3 minutes in a PCR machine (Bio-Rad), with the temperature range being 42-64 °C and 2-degree intervals ...
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bioRxiv - Biochemistry 2020Quote: ... rinsed 3 times and read by the Bio-Plex (Bio-Rad, Bio-Plex MAGPIX Multiplex Reader). Each experiment was done in duplicate and repeated with media from 3 independent experiments (n=3).
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bioRxiv - Systems Biology 2020Quote: ... The samples were digested for 3 hours in a thermal cycler at 37 °C (BioRad T1000). Samples were cooled to room temperature before TMT labeling (TMT11 plex kit & TMT-Pro 16plex ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted using a 20:7:3 mixture of buffer: 4x Laemmli sample buffer (Biorad):10x NuPage sample reducing agent (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... washed again 3 times in TBS-T and visualized in a ChemiDoc Imaging System (Bio-Rad). Antibodies were diluted in antibody solution containing 1% non-fat milk in TBS-T ...
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bioRxiv - Biochemistry 2021Quote: ... For radioactive samples, gels were dried (80°C, 3 hr) on a gel dryer (Bio-Rad), exposed to a phosphor screen ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol) 3 times and moved into a 25 mL Econo-Column Chromatography Column (Bio-Rad), where they were further washed with a minimum of 200 mL of wash buffer ...
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bioRxiv - Bioengineering 2022Quote: BP-PA hydrogel precursor solution contained 3-7.5% acrylamide (Am, 40% w/v stock, 161042, BioRad), 0.035-0.25% N,N-methylenebisacrylamide crosslinker (Bis ...
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bioRxiv - Neuroscience 2022Quote: ... was used for visualizing RIC-3 signal with a digital imaging system (ChemiDoc MP, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... 3 final 10-minute TBST washes were followed by visualization of proteins with ECL (BioRad, 11705062). Densitometry analysis was performed using ImageLab 6.0 software (BioRad ...
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bioRxiv - Immunology 2023Quote: Fab (3 μg/lane) was loaded on a 4%-12% Bis-Tris precast gel (Bio-rad) in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated in blocking buffer consisting of 1X TBS-T + 3% Blotting-Grade Blocker (BioRad) shaking at room temperature for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... at 2.5 Å for 3 minutes using Trans-Blot® Turbo™ Transfer System (Biorad 1704150). The membranes were blocked at room temperature for 1 hour in Odyssey blocking buffer (LiCor ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated in blocking buffer consisting of 1X TBS-T + 3% Blotting-Grade Blocker (BioRad) shaking at room temperature for 1 hr ...
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bioRxiv - Bioengineering 2023Quote: ... and Glyceraldehyde-3-Phosphate Dehydrogenase (Gapdh) were determined using SsoFast™ EvaGreen® Supermix (Bio-Rad), quantified using CFX96 C1000 thermocycler (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Glycerinaldehyde-3-phosphate dehydrogenase (GAPDH) RNA was used as reference gene (Bio-Rad, assay ID: qRnoCID0057018).
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bioRxiv - Neuroscience 2023Quote: ... Glycerinaldehyde-3-phosphate dehydrogenase (GAPDH) was used as a reference gene (Bio-Rad, assay ID: qRnoCID0057018).
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bioRxiv - Cell Biology 2024Quote: ... washed 3 × 10 min with TBST and exposed with a ECL kit (Bio-rad, cat # 1705061) on X-ray films (Kodak ...
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bioRxiv - Microbiology 2024Quote: ... 10% glycerol) then boiled with 1/3 volume of 4x Laemmli sample buffer (Bio-Rad, 1610747) for 10 mins ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cytokine levels were measured using the magnetic bead-based Bio-Plex Pro Human cytokine 27-Plex (Bio-Rad) according to manufacturer’s instructions and measured the cytokines using the Bio-Plex 200 instrument ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analyzed in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Quantification of band intensities in 3 Western blot replicates was performed using Image Lab software (Bio-Rad). PALS-5 band intensities for each sample were normalized to the ratio of the tubulin expression levels between N2 DMSO control and a given sample.
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Microbiology 2019Quote: ... A 3 μl volume of this master mix was loaded into a 384 well plate (BioRad HSP3805) using a multichannel pipette according to the layouts in Figure 2 ...
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bioRxiv - Neuroscience 2020Quote: ... SDS-gels (3% stacking gel, 12% separation gel) were run in a Mini PROTEAN® System (BioRad) at 100 V for 10 min and 200 V till end of the separation ...
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bioRxiv - Systems Biology 2020Quote: Plates were incubated for 2-3 days at 30 °C and imaged using a ChemiDoc MP (BioRad). Individual colonies from the plates generated to enable high second stress survival quantitation were counted using in-house developed MATLAB scripts ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3 times and imaged by chemiluminescence (Pierce) by using a Chemidoc imaging system (BioRad). The images were further analyzed for band intensities using ImageJ software ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates from 3×106 cells were separated on 4-20 % gradient Tris-glycine polyacrylamide gels (BioRad 4568094), electroblotted onto a PVDF membrane (Pierce 88518) ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 M NaCl) for 30 minutes and the DNA was transferred to a Zetaprobe membrane (Bio-Rad) in 20X SSC ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified product was run on 3% certified™ low range ultra agarose (Bio-Rad Laboratories Ltd) in TBE buffer and a size-selection was performed to ensure that the cDNA used for sequencing primarily contains miRNAs rather than other RNA contaminants ...
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bioRxiv - Immunology 2020Quote: ... and detergent removed by adsorption to polystyrene BioBeads SM-2 (3 mg; Bio-Rad; overnight, 4°C). Proteoliposomes were collected by ultracentrifugation (180,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... the blot was rinsed 3 times in TBS-T and incubated with StrepTactin-HRP conjugate (Bio-Rad) and HRP-conjugated secondary antibody for 1 hour at 22°C ...
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bioRxiv - Microbiology 2020Quote: ... or 500 ng to 3 µg for integrative plasmids (MicroPulserTM electroporator Biorad in 2 mm cuvettes (Eurogentec) at 25 µF ...
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Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... 10–15 μL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V in 1x Tris-Acetate buffer ...
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bioRxiv - Immunology 2022Quote: ... 3 technical replicates per sample were measured on a CFX384 Touch Real-Time PCR Detection System (Biorad). Cycles were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... 10–15 µL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V to separate Rad53 isoforms ...
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bioRxiv - Molecular Biology 2022Quote: ... and added to 7 cm ReadyStrip IPG Strips with a linear pH range of 3-10 (BioRad) for passive rehydration overnight at 20 °C in a Protean IEF Cell equipment (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Isoelectric focusing was performed in immobilized pH gradient strips (17 cm, pH 3–10 NL; Bio-Rad). The focused strips were stored at −80°C until second-dimension electrophoresis was performed ...
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bioRxiv - Plant Biology 2023Quote: ... Leaves from 3 dai (days after infiltration) were used for GFP detection by ChecmiDoc MP machine (BioRad), and ImageJ Fiji was used for quantification of GFP intensity ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBST 3 times and imaged using the ChemiDoc MP imaging system (Bio-Rad) after activation with ECL substrate solution.
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bioRxiv - Molecular Biology 2023Quote: ... ∼3 million cells were harvested and proteins were extracted by boiling in 2x Laemmli sample buffer (BioRad). Protein levels were measured by Western blotting with different antibodies (Rabbit anti- BRWD3 ...