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Citations for Bio-Rad :
251 - 300 of 467 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Molecular Biology 2020Quote: ... The blots were washed three times with TBS-T for 5 min each time before to development with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging on the ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed in PBS-T for three times 5 min and developed through chemiluminescence using a ChemiDoc XRS (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity-ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using oligo-d(T) primers and avian reverse transcriptase (iScript cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... Blots were washed four times in TBS-T prior to detection with either ProSignal Dura (Prometheus) or Clarity ECL (Bio-Rad) substrate and exposure to radiography film or imaging on a C-Digit imager (LiCOR).
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bioRxiv - Microbiology 2023Quote: ... Blots were washed three more times in TBS-T before being developed with Clarity Max Western ECL reagent and imaged on a ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The blots were washed 3 times in PBS-T and the proteins were visualized using Clarity Western ECL blotting substrate (Bio-Rad) or SuperSignal West Femto (ThermoScientific) ...
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bioRxiv - Microbiology 2023Quote: Membrane was then washed three times with TBS-T and incubated with secondary antibody (Goat Anti-Rabbit IgG StarBright Blue 700 by Bio-Rad) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Physiology 2023Quote: ... The membranes were washed in TBS-T and developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo) in a ChemiDoc imager (Bio-Rad). We normalized the protein bands to beta-tubulin with Image Lab software (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was washed three times with T-TBS and twice with TBS for 5 min each and incubated with Clarity Western ECL substrate (Bio-Rad). After 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and chemiluminescence was assessed with the BioRad ChemiDoc MP Imaging System (BioRad, 12003154).
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was amplified using KAPA HiFi Hotstart ReadyMix PCR kit (Nippon Genetics) with T-100 Thermal Cycler (Biorad, Hercules, CA). The temperature profile of the reaction was 95 °C for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...
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bioRxiv - Genetics 2021Quote: Each 25 μL ddPCR reaction contained 12.5 μL 2x Supermix for Probes (no dUTP) (Bio-Rad Laboratories, #186-3024), cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of protein was then fractionated on Tris–glycine SDS-PAGE gradient (4–15% acrylamide) gels (BioRad; #3450123), transferred onto nitrocellulose membranes (Millipore) ...
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bioRxiv - Developmental Biology 2021Quote: ... was performed in 7 min at 1.3 A and 25 V using a Trans-Blot TurboTM transfer system (BioRad). Recognition and revelation of the his-tagged protein was performed as described for the Dot Blot ...
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bioRxiv - Biochemistry 2022Quote: ... Before the protein transfer was carried out 45 min at 25 V using a Semi Dry Blotter (Bio-Rad), the transfer membrane was pre-incubated 5 min in methanol ...
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bioRxiv - Microbiology 2019Quote: The 25□μl qPCR reactions contained 12.5□μl iQ™ SYBR® Green Supermix (Bio-Rad, Hercules, California, US), 1□μl of each forward and reverse primers ...
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bioRxiv - Genetics 2020Quote: ... Protein gels were transferred to a 0.4-μm nitrocellulose membranes at 1.3 A and 25 V for 15 to 20 minutes in a semi-dry apparatus (BioRad). After protein transfer ...
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bioRxiv - Microbiology 2019Quote: The 25□μl qPCR reactions contained 12.5□μl iQ™ SYBR® Green Supermix (Bio-Rad, Hercules, California, US), 1□μl of each pM forward and reverse primers ...
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bioRxiv - Cell Biology 2019Quote: ... The YFP signal intensities were quantified using Image Lab™ version 6.0.0 build 25 Standard Edition (Bio-Rad Laboratories). High molecular weight regions were defined ...
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bioRxiv - Cancer Biology 2020Quote: ... 25 μg of protein was then fractionated on Tris–glycine SDS-PAGE gradient (4–15% acrylamide) gels (BioRad; #3450123), transferred onto PVDF membranes (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... The BRPF1 bromodomain protein was purified on glutathione agarose resin in a 25 mL Econo-Column® (Bio-Rad) by first washing the beads extensively with wash buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The temperature was varied 25 - 95 °C in 0.5 °C increments by using an iCycle IQ5 real-time PCR system (BioRad). Relative fluorescence was measured using excitation and emission wavelengths of 580 nm and 623 nm ...
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bioRxiv - Microbiology 2021Quote: ... Mab competent cells were incubated on ice with ~500 ng of DNA for 10 minutes and transformation was performed with the following settings (2.5kV, 25 uF, 1000ohms) using an electroporator (BioRad). Cells were recovered in 1ml of Middlebrook 7H9 broth incubated for 4 hrs at 37°C and inoculated onto Middlebrook 7H10 agar supplemented with appropriate antibiotics ...
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bioRxiv - Cell Biology 2022Quote: ... the tubes were dialyzed twice for 48-72h against buffer I (25mM Tris pH 7.4, 25 mM NaCl, 1 mM β-mercaptoethanol and 0.5 g of Bio-Beads (Biorad). After dialysis ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... 25 µg of total protein was separated on 4-20% Criterion TGX precast midi protein gels (Bio-Rad #5671094) and transferred onto nitrocellulose membranes (Thermo Fisher Scientific #IB23001 ...
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bioRxiv - Genetics 2022Quote: ... PMOs were buffer exchanged into 25 mM ammonium bicarbonate buffer pH 7.5 using Biospin 6 (Bio-rad, Hercules, California) columns according to the manufacturer’s protocol and then spinning through an 0.22 µm cellulose acetate centrifugal spin filter ...
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bioRxiv - Neuroscience 2019Quote: ... 50 μg of target DNA was precipitated onto 25 mg of 1.6 μm diameter gold microcarriers and bullets generated in accordance with the manufacturer’s instructions (Bio-Rad). At the time of recording ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA template was diluted 25 times and subsequently used for qRT-PCR employing iQTM SYBER Green supermix (Bio-Rad) on a BioRad CFX96 Real-Time PCR machine ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 hpf (n=40) or 48 hpf (n=25) embryos using the Aurum Total RNA Mini Kit (Bio-Rad). cDNA was then prepared with the RevertAid reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein transfer was performed in transfer buffer (0.192 M glycine, 25 mM Tris base, 20 % EtOH in ddH2O) in a TransBlot Turbo System (BioRad). After the transfer all proteins were visualized by Ponceau staining ...
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bioRxiv - Microbiology 2020Quote: ... previously activated by ethanol and pre-equilibrated with transfer buffer (25 mM Tris, 192 mM glycine, 0.1% SDS, 20% ethanol) with a Trans-Blot Turbo system (BioRad). The PVDF membrane was blocked for 1 h in PBS supplemented with 0.1 % Tween-80 from Sigma Aldrich (‘PBS-Tween’ ...
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bioRxiv - Microbiology 2020Quote: ... 25 µg of protein was run per sample on 4-15% Mini-PROTEAN TGX stain-free precast gels (BioRad) at 150V ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25 percent of beads by boiling in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from 75 percent of beads using TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... a standard starting volume of approximately 25 μL of DNA was transferred to a 96-well plate (BioRad, HSP9601). 1μL of unnormalized DNA was removed and transferred to a black 96-well plate (VWR ...
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bioRxiv - Microbiology 2022Quote: ... in water and 3.75 μL of sample was mixed with 0.18 μL of 25 mg/mL primers and 7.5 μL of iQ SYBR master mix (Bio-Rad). 15 μL reactions were analyzed with a CFX connect real-time PCR detection system in technical duplicate and at least biological triplicate ...
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bioRxiv - Microbiology 2022Quote: 25 μl of the secreted proteins were ran on a Mini-PROTEAN® TGX™ precast gel (Bio-Rad). The whole lane of the gel for each sample was sliced into 4 mm sections and washed with 1 ml of 50% ethanol in 50 mM ammonium bicarbonate (ABC) ...
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bioRxiv - Neuroscience 2023Quote: ... 25 µl cell lysate and 50 µg protein for tissue lysates was loaded on 4-15% (Bio-Rad, 5678084) and 10% TGX Stain-Free Protein Gels (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes via semi-dry transfer (Bio-Rad Trans Blot Turbo; 25 V, 30 min) for western immunoblot analysis ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL of cells were added to an electroporation cuvette (0.1 cm gap, Bio-Rad, catalog no. 165-2089), and electroporated using the Ec1 setting (1.8 kV ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl of non-boiled protein sample were loaded on 12% Mini-Protean TGX pre-cast gels (Bio-Rad) and the proteins separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was carried out at 80-150 V constant voltage per gel in running buffer (25 mM Tris·Cl, 250 mM glycine, 0.1% SDS) in a minichamber (MiniPROTEAN System, BIO-RAD).
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bioRxiv - Cell Biology 2023Quote: ... Proteins (25 μg/lane) were separated on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). For the detection of α- syn ...