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Citations for Bio-Rad :
601 - 650 of 1299 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Approximately 90-100 μg of each sample was loaded on 4-20% precast gradient gel (Bio-Rad) and resolved proteins were transferred to a PVDF membrane ...
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bioRxiv - Neuroscience 2019Quote: ... 100 ng Total RNA was converted to cDNA using a iScript™ cDNA Synthesis Kit (Bio-Rad). qRT-PCR was performed on an CFX96 real-time PCR detection system in 10 μL reactions containing SsoAdvanced™ Universal SYBR® Green Supermix and 200-300 μM of primer and 1 μL of 1:5 diluted cDNA in technical triplicates ...
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bioRxiv - Immunology 2021Quote: ... cDNA was diluted 1 μg / 100 ul and qPCR was performed on a CFX96 Instrument (Bio-Rad) using SYBR Green incorporation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted using 250 μL of 5% Chelex 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng RNA was reverse-transcribed into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad, #1708841). Minigene isoforms were amplified by Taq PCR from 2 uL cDNA using forward and reverse primers complementary to the vector sequence (RG6_F ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction from heads was performed using the Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) [35] as adapted in Shi et al ...
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bioRxiv - Microbiology 2022Quote: ... the minimal medium was treated with 5% Chelex-100 (Bio-Rad, cat. No. 142-2842, Hercules, CA) for 24 h with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Cell Biology 2023Quote: ... and the electrophoresis was run at 100 V (by PowerPac HC High-Current Power Supply, Bio-Rad) in a Mini-PROTEAN Tetra Electrophoresis Cell (Bio-Rad) ...
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bioRxiv - Pathology 2023Quote: ... at a dilution of 1:100 and fibrinogen using the monoclonal anti-fibrinogen antibody (#4440-8004, BioRad) at a dilution of 1:200 incubating over night at 4° C in a humidified chamber ...
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bioRxiv - Biophysics 2022Quote: ... DDM was removed by addition of a 100-fold excess (w/w) of biobeads SM-2 (Biorad) compared to protein for 4 h at 4 °C under gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Bound proteins were then eluted through boiling at 100°C in Laemmli sample buffer (BIORAD, CA, USA). The bound proteins were eluted at 37°C for 10 min.
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bioRxiv - Physiology 2023Quote: ... and the electrophoresis was run at 100 V (by PowerPac HC High-Current Power Supply, Bio-Rad) in a Mini-PROTEAN Tetra Electrophoresis Cell (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... and the electrophoresis was run at 100 V (by PowerPac HC High-Current Power Supply, Bio-Rad) in a Mini-PROTEAN Tetra Electrophoresis Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately 100 ng of mRNA was reverse transcribed into cDNA using iScript cDNA Supermix (Bio-Rad, 1708890) and amplified using specific primers listed in Supplementary Table 1 ...
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bioRxiv - Genetics 2023Quote: ... and orange G) and ran for 3 h at 100 V with a Variable Speed Pump (BioRad) to circulate buffer during the entire gel run.
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bioRxiv - Molecular Biology 2024Quote: ... and transferred onto PVDF membranes using a wet blot system for 1 h at 100 V (Biorad). Membranes were blocked with BSA and incubated with primary antibody overnight at 4° C ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of isolated RNA per reaction was utilized with the iScript cDNA Synthesis System (Bio-Rad). The resulting cDNA served as the template for quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Neuroscience 2024Quote: ... or at 100 V for 2 h at 4 °C using the wet transfer method (Bio-Rad Mini Trans-Blot Electrophoretic Cell 170-3930) ...
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bioRxiv - Plant Biology 2021Quote: ... Two mL of juice was gently pushed through a one mL column packed with ion exchange resin (Bio-Rad; Mixed bed resin 272 AG501-X8) and then freeze-dried ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mL of 1x PBST + 5% blotting grade blocker (Bio-Rad) were added for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... Rat Anti-mouse CD68 (Biorad, MCA1957T, 1:200 dilution, 5μg/mL); Rabbit polyclonal anti-Histone-H2B (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... each 10 ml fraction was tested with Bradford reagent (Bio-Rad) for protein content until no more protein could be detected ...
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bioRxiv - Cell Biology 2021Quote: ... After DAPI staining (1 μg/ml, Bio-Rad, Hercules, CA, USA), adult fly brains were mounted in PermaFluor (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The resin was loaded onto a 20 ml econo-column (BioRad) and extensively washed with increasing concentrations of imidazole in PBS (10 ...
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bioRxiv - Biochemistry 2022Quote: ... and the lysate was run over 1 mL columns (Biorad, 7321010) packed with Ni-NTA Resin (VWR ...
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bioRxiv - Cell Biology 2019Quote: ... and droplet generation oil (Bio-rad, 1864006; 7 ml per run), were connected to a microfluidics device (FlowJEM ...
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bioRxiv - Molecular Biology 2020Quote: ... added to a 10-ml poly-prep chromatography column (Bio-Rad) and the wash buffer allowed to flow through the column ...
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bioRxiv - Microbiology 2020Quote: ... were coated with 1 μg/ml of anti-CH3 (MCA878G, BioRad) in 1x PBS with 100 μl/well and plates were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Assays were stained with 10 μg/mL ethidium bromide (Bio-Rad) in PBS before assessment of parasitaemia using flow-cytometry (BD Biosciences LSR II ...
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bioRxiv - Genomics 2021Quote: ... anti-CD68 (5 µg/mL, MCA341R, ED1, mouse IgG1, Bio-Rad), or anti-Collagen 1α1 (1 µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 2 ml bed volume (0.8 × 4 cm) empty polypropylene column (BioRad) fitted with a two-way stopcock up to the fill line ...
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bioRxiv - Cell Biology 2020Quote: ... packed on 1 ml Empty Bio-Scale Mini Cartridges (Biorad #7324660). The column was then washed with buffer at pH 7.5 (25mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... and concentration adjusted to 5.5 mg/mL using Bradford assay (BioRad). 1 % DDM was then added ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated cell types were further lysed in 1 ml Purezol (Biorad) to proceed with RNA extraction.
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bioRxiv - Physiology 2023Quote: ... and rat anti-mouse cd68 (5 µg/mL, MCA1957, Bio-Rad) diluted in PTxwH buffer for 4 days ...
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bioRxiv - Biophysics 2021Quote: ... with a cut-off of 100 kDa and subjected to SEC using an EnRich 650 column (Bio-Rad) and an Agilent LC-1220 system in 20 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... TBST (50 mM Tris-HCl pH 7.4, 1% Triton X-100) with 5% non-fat milk (Bio-Rad) was used for blocking ...
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bioRxiv - Developmental Biology 2020Quote: ... 100 ng of RNA was used for first strand cDNA synthesis using the iScript Supermix (Bio-rad, 1708841). cDNA was diluted 18-fold and 4 µL was used in a reaction for SYBR green detection with SsoAdvanced SYBR Green supermix (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... first using Qiagen DNeasy and again with a 10% Chelex 100 (200-400 mesh; Bio-Rad, Hercules, CA) solution containing 1% each of Nonidet P-40 and Tween 20 (Millipore Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were eluted by boiling 10 min in 100 µL H2O containing 10% (V/W) chelex resin (BioRad), PK-digested for 30 min and then finally incubated 10 min at 95°C ...
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bioRxiv - Molecular Biology 2019Quote: ... we used a predesigned Cholangiocarcinoma Pathway Panel (88 targets) (PrimePCR, Bio-Rad, Hercules, CA, catalog no. 100-2531). One microgram of total RNA from each exosome sample was converted to cDNA (Supermix iScript kit ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were washed with ice-cold PBS and lysed by using 100 μL lysis buffer (Bio-Rad). After confirming lysis by light microscopy ...
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bioRxiv - Microbiology 2020Quote: ... 100 - 1,000 ng of total RNA was used for cDNA synthesis using iScript cDNA synthesis kit (Bio-Rad). RT-qPCR was performed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Immunohistochemistry was performed on paraffin sections (5 μm) using antibody F4-80 (Bio-rad, France; diluted 1:100). Sections were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Isothermal amplification was carried out at 65 °C for 30 min on a T-100 Thermocycler (Bio-Rad), according with manufacturer conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... for electrophoresis at 100-120 volts followed by semi-dry electrophoretic transfer to nitrocellulose membranes (Bio-Rad #1704270) via Bio-Rad Trans-Blot Turbo transfer system ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 μg of each sample was loaded into the 4–20% gradient precast SDS-PAGE gels (BioRad).
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bioRxiv - Microbiology 2021Quote: ... Approximately 100 μL 0.1 mm diameter zirconium beads and 180 μL of 1x Laemmli Sample Buffer (Bio-Rad) were added to 2 mL screw cap micro tubes (Sarstedt Inc) ...
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bioRxiv - Immunology 2022Quote: ... calcium in fetal bovine serum (FBS) was depleted by incubation with Chelex 100 resin (BioRad, Hercules, CA, USA) for 1 h at 4 C ...