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Citations for Bio-Rad :
101 - 150 of 231 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Table A in S1 Text) in a single reaction using a CFX Opus real-time qPCR machine (CFX96, Bio-Rad Laboratories, CA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was then generated using the Superscript™ III Reverse Transcriptase kit and pre-amplified with a mixture of the tested TaqMan® probes (Table S1) using the SsoAdvanced™ PreAmp Supermix (Biorad). Differential levels of cDNA expression were measured using the droplet digital PCR (ddPCR ...
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bioRxiv - Immunology 2021Quote: ... TaqMan gene expression Master Mix (Applied Biosystem) was used to determine the cytokine (Table-S1) expression levels in a CFX96 Touch Real-Time System (BioRad, Hercules, CA). The data were analyzed with CFX Manager Software ...
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bioRxiv - Immunology 2023Quote: Gene mRNA expression was determined by using primers in Table S1 with a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories) following reverse transcription with SuperScript Reverse Transcriptase (18064022 ...
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bioRxiv - Microbiology 2023Quote: ... coli DH5α using a Bio-Rad Gene Pulser II Electroporation system (Bio-Rad) with the following parameters ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 detection was performed on the CFX96™ Real-Time PCR Detection System (Bio-Rad, California, USA), using the NeoPlex™ COVID-19 Detection Kit (Genematrix ...
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bioRxiv - Molecular Biology 2022Quote: ... The SARS-COV-2 NTD expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences) ...
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bioRxiv - Immunology 2020Quote: ... was purchased from G-Biosciences and Freeze ‘N Squeeze DNA gel extraction columns by Bio-rad, Inc ...
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bioRxiv - Biophysics 2022Quote: ... which were subsequently used with Freeze ‘N Squeeze spin columns (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... The cDNA was amplified by qPCR with specific primers (Supplementary Table S1) using the C1000™ thermal cycler with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories). The relative expression of genes was analyzed quantitatively by the ΔΔCt method ...
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bioRxiv - Plant Biology 2022Quote: ... with 200 nM of each gene-specific forward and reverse primer (Supplemental Table S1) mixed with SYBR® Green supermixes (Bio-Rad, USA) according to the manufacturer’s instructions on a Thermal Cycler CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... immunoprecipitated DNA samples were analyzed using primers described in Table S1 in technical triplicate using iTaq Universal SybrGreen reagents (BioRad, Hercules, Ca, USA) in a RealPlex Instrument (Eppendorf ...
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bioRxiv - Plant Biology 2023Quote: Real-time quantitative PCR was performed using gene-specific primers (Table S1) for selected genes on CFX96 Touch™ Real-Time PCR Detection System (Biorad, California, USA). The amplification conditions were ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was diluted 10 fold in nuclease free water and reactions were performed in a Bio-Rad CFX96 system using gene specific primers (Table S1) and Bio-Rad SYBR green master mix (Bio-Rad, Hercules, USA). Each sample was run in triplicate ...
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bioRxiv - Microbiology 2024Quote: ... blaOXA-48) and the intI1 gene by PCR methods 20-23 (Supplementary tables S1 and S2) in a Thermocycler (BIO-RAD T100™) using specific primer sets (Supplementary table S2) ...
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bioRxiv - Microbiology 2020Quote: ... coli strain S17-1 using a Gene Pulser Xcell™ (Bio-Rad, CA, USA) with the conditions of 2.5kV ...
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bioRxiv - Microbiology 2022Quote: ... coli CA434 cells by electroporation using a Gene Pulser Xcell Electroporation System (Bio-Rad Laboratories Ltd ...
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bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted as described above, followed by RT-PCR using the Allplex SARS-CoV-2 assay (SeeGene, South-Korea) on a CFX96 thermocycler (Biorad), simultaneously detecting four different target genes ...
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bioRxiv - Biochemistry 2022Quote: ... blots were imaged using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Biochemistry 2022Quote: ... Protein was detected using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Plant Biology 2020Quote: Relative expression of CgCOM1 transgene in chilli- and tomato-CgCOM1i transgenic lines was analyzed by RT-qPCR using CgCOM1 gene specific primers (Supplementary Table S1) and SSoFast™ EvaGreen® supermix (Bio-Rad, USA) on CFX96 Touch™ Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: Protein overexpression and purification were analysed via 10% SDS-PAGE and imaged via ChemiDoc™ MP Imaging System (Bio-Rad; Fig. S1 and S4). Immunoblotting was performed to confirm purification results with primary anti-6xHis polyclonal mouse antibody (Beyotime® ...
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bioRxiv - Developmental Biology 2022Quote: ... HRMA is a powerful tool to screen indel mutations or single nucleotide polymorphism in the samples and it was performed on the genomic DNA with specific primers listed in Table S1 using the CFX96 Real-Time PCR Detection System and the Precision Melt Analysis software (Bio-Rad Laboratories, Hercules, CA). HMA was performed with PAGE to verify the genotyping results of HRMA ...
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bioRxiv - Molecular Biology 2023Quote: ... final concentrations of 2X SsoADV Universal SYBR Green Supermix (BIORAD, n° 1725270) and 0.04μM of each primer ...
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bioRxiv - Biophysics 2023Quote: ... and samples were extracted with Freeze N’ Squeeze spin columns (Bio-Rad) by centrifugation at 1,000 g for 60 minutes at 4 ºC ...
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bioRxiv - Immunology 2022Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Optimized ddPCR was used to detect the presence of SARS-CoV-2 viruses following our previous study.10 Analysis of the ddPCR data was performed with QuantaSoft software (Bio-Rad). The concentration reported by the procedure equals copies of template per microliter of the final 1x ddPCR reaction ...
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bioRxiv - Immunology 2021Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral copy numbers were determined using SARS-CoV-2 RdRp gene specific primers and probe (see [28]) and droplet digital PCR (Bio-Rad Laboratories Inc. ...
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bioRxiv - Microbiology 2020Quote: ... coli isolates was performed by macrorestriction with XbaI followed by PFGE (Chef DRII, Bio-Rad), according to the PulseNet protocol with pulses oscillating from 2.16 to 63.8 s for 21.5 hours (Ribot et al. ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells and purified using immobilized metal affinity chromatography and a Profinia Purification System (BioRad) and size exclusion chromatography via an ÄKTA fast protein liquid chromatography system (GE Healthcare) ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR was conducted with primers indicated in Table S1 and ChamQ Universal SYBR® qPCR Master Mix (Vazyme, China) on a Bio-Rad CFX384 qPCR cycler (Bio-Rad Laboratories, Shanghai, China).
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bioRxiv - Genomics 2022Quote: ... Each sample was tested by PCR systems (threshold – 0,050) to the presence of SARS-CoV-2 using a CFX Connect Real-Time PCR System (BioRad, Hercules, California, USA). RT-PCR were implemented in 40 μl of final volumes ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd) ...
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bioRxiv - Genetics 2023Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd.) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd.) ...
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bioRxiv - Immunology 2023Quote: Serum SARS-CoV-2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS-CoV-2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... crushed and structures were purified with Freeze ‘N Squeeze spin columns (Bio-Rad) for 5 min at 1,000×g at 4 °C.
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bioRxiv - Biophysics 2020Quote: ... crushed and spun through a Freeze N’ Squeeze column (BioRad Sciences, 732-6165) for 3 min at 13,000g at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Freeze ‘N Squeeze columns (catalog no. 732-6165) were obtained from Bio-Rad. Cy3b-maleimide (catalog no ...
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bioRxiv - Immunology 2024Quote: ... filtered through Freeze ‘N Squeeze DNA Gel Extraction Spin Columns (Bio-Rad; #7326165) and purified via ethanol precipitation ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Biochemistry 2022Quote: Supernatants from Wuhan SARS CoV-2 infected cells prepared as described above or uninfected cells and commercial spike were lysed with 2x Laemmli Sample Buffer (#1610737, Bio-Rad, Hercules, CA, USA) with DTT (#EU0006-B ...
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bioRxiv - Systems Biology 2020Quote: ... coli K12 whole cell lysates were analysed using 10% Mini-PROTEAN Protein Gels (4568034, Bio-Rad, Laemmli buffer S3401-VL ...
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bioRxiv - Microbiology 2020Quote: ... coli isolates was extracted using the InstaGene™ Matrix following manufacturer’s guidelines (Bio-Rad, Montreal Canada). For all multiplex PCR assays ...
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bioRxiv - Microbiology 2021Quote: ... coli strain S17-1 using a Gene Pulser Xcell™ (Bio-Rad, Hercules, CA, United States) with the conditions of 2.5kV ...