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Citations for Bio-Rad :
1 - 50 of 139 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Plasmids were transferred to C. sporogenes by conjugation as described before (Heap et al., 2009) using E. coli CA434 (E. coli HB101 (Bio-Rad Laboratories, The Netherlands) harboring the broad host IncPß+ conjugational plasmid pRK24 (Williams et al. ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-SARS CoV-2 Spike Neutralizing antibody (SAD-S35, ACRO, USA) or anti-Human IgG Kappa (STAR 127, Bio-Rad, USA) antibody ...
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bioRxiv - Cell Biology 2022Quote: ... and through mosaic ratio quantification by ddPCR Technology (Bio-rad’s QX100tm/Copy Number Assay) at different indicated time points ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Microbiology 2023Quote: Electroporations of A. baumannii were performed using 0.1cm gap cuvettes (0.2cm for E. coli) and the Gene Pulser (Bio-Rad) at the settings 200 Ω ...
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bioRxiv - Plant Biology 2022Quote: ... Finished CRISPR/Cas9 binary vectors were transformed into Agrobacterium tumefaciens EHA105 (Hood et al., 1993) by electroporation (E. coli Pulser, BioRad).
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bioRxiv - Cell Biology 2023Quote: ... Episome plasmids were transformed into E. coli (TransforMax EPI300) harboring the pTA_Mob51 mobility plasmid (gift from R. Lale) via electroporation (Bio-Rad). Transformed cells were spread onto LB agar plates containing both gentamycin (10 μg mL-1 ...
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bioRxiv - Plant Biology 2021Quote: ... The binary vector pMGE599 was introduced into Agrobacterium tumefaciens AGL-1 strain (62) through electroporation (E. coli Pulser; Bio-Rad, http://www.bio-rad.com). The scutella tissue of barley caryopsis was used for Agrobacterium-mediated transformation as described previously (63) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Initial screening for IgG antibodies was undertaken using an extensive panel of bacterial (Leptospira, Brucella) and viral antigens (paramyxovirus, filovirus, coronavirus, flavivirus, alphavirus) coupled to MagPlex beads (Bio-Rad, Australia) for multiplex screening ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Microbiology 2021Quote: ... coli Pulser (Bio-Rad). Electroporation was performed under same conditions as previously described47 ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... coli antibodies (BioRad, ON) or rabbit anti-Salmonella O antiserum group B antibodies (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Molecular Biology 2020Quote: Predesigned overlapping oligodeoxynucleotides (Fig S1) were assembled by PCR (BioRAD T100) using Primers F1 ...
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bioRxiv - Molecular Biology 2023Quote: ... a 160kD glycoprotein expressed by murine macrophages 235 (1:100, Bio-Rad clone C1:A3-1, Cat. MCA497) and rabbit anti-laminin (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
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bioRxiv - Bioengineering 2021Quote: ... Synthetic SARS-CoV-2 RNA was purchased from BIORAD (SARS-CoV-2 Standard #COV019 and SARS-CoV-2 Negative #COV000).
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bioRxiv - Microbiology 2020Quote: ... coli strain NEB10ß (Bio-Rad) carrying the BAC was electroporated with the pRed/ET expression plasmid using an Eppendorf Eporator® (Eppendorf ...
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bioRxiv - Immunology 2024Quote: ... coli using the Micropulser (BioRad) with pulse setting Ec1 ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2022Quote: ... Primer nucleotide sequences (Table S1) were determined using Beacon Designer software (Bio-Rad) and their specificity was analyzed by BLAST and PCR dissociation curve analysis ...
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bioRxiv - Cell Biology 2021Quote: ... coli antibody (1:100, Bio-Rad), anti-LAMP1 (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... coli Pulser™ Transformation Apparatus (BioRad) was used to electroporate One Shot™ TOP10 Electrocomp™ E ...
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bioRxiv - Plant Biology 2020Quote: ... using gene specific primers (Table S1) and iTaq Universal SYBR Green Supermix (Bio-rad). The actin gene (OsAct1 ...
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bioRxiv - Biophysics 2020Quote: ... coli RNAP (Bio-rad, Hercules, CA, USA) to a final concentration of 45.5 nM ...
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bioRxiv - Microbiology 2022Quote: ... coli Gene Pulser (Bio-Rad, Hercules, CA). All pDN201-derived plasmids were verified by Sanger sequencing at the UC Berkeley DNA Sequencing Facility and all pAMG40 cointegrates were verified by restriction endonuclease analysis.
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bioRxiv - Microbiology 2023Quote: ... coli settings (1.8 kV, Bio-Rad MicroPulser). With a 1000 µL pipette ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad). Samples were processed as technical triplicates with the number of independent experimental replicates reported in the figure legends ...
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bioRxiv - Microbiology 2020Quote: ... coli Dh5α using IProof DNA polymerase from BioRad and restriction enzymes from New English Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... coli Gene Pulser® (Biorad, Hercules, CA, USA), as recommended by the supplier ...
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bioRxiv - Biophysics 2023Quote: ... coli was purchased from Bio-Rad (Hercules, CA) and received as a lyophilized powder ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Biophysics 2021Quote: ... coli lysate was purchased from Bio-Rad (Hercules, CA) and received as a lyophilized powder ...
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bioRxiv - Microbiology 2021Quote: ... coli SM10 λpir by electroporation (MicroPulser Electroporator, Bio-Rad). The parental E ...
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bioRxiv - Cell Biology 2021Quote: ... coli antibodies (1:100, Bio-Rad Antibodies, 4329-4906), followed by DyLight 650-conjugated donkey anti-rabbit IgG antibodies (1:1000 ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein eluates were adjusted to 250 mM NaCl and loaded onto a Uno S1 (Bio-rad) ion exchange column ...
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bioRxiv - Cell Biology 2021Quote: ... Target gene cDNA was amplified using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad) on a CFX384 Touch Real-Time PCR Detection system (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT PCR was performed with gene-specific primers (Table S1) using iTaq universal SYBR Green Supermix (BioRad) for 45 cycles on CFX96 Real time system (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... The post-nuclear S1 fraction (supernatant) was collected and protein concentration measured using the BCA protein assay (Biorad).
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bioRxiv - Molecular Biology 2021Quote: Purified SARS-CoV-2 N protein was electrophoresed on 4-10% SDS-PAGE (Bio-Rad) and stained with Coomassie Brilliant Blue G-250 (CBBG-250) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli recipient cells via electroporation with a MicroPulser (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...