Labshake search
Citations for Bio-Rad :
101 - 150 of 1243 citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... concentration was fixed at 5% while N,N-methylene-bis-acrylamide (bis, Bio-rad) varied from 0.04% to 0.1% to attain different stiffnesses of the substrates ...
-
bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-V5 tag antibody (SV5-Pk1, Bio-Rad) and secondary antibodies (Goat anti-Mouse IgG (H+L ...
-
bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
-
bioRxiv - Cell Biology 2020Quote: ... FCS was chelated with Chelex-100 resin (BioRad) and added to Ca2+ free FAD medium ...
-
bioRxiv - Molecular Biology 2021Quote: ... The amount of used Pol3 CysA + CysB domains were detected by UV-imaged Criterion gels (BioRad) and the binding of Pol31 to Pol3-CTD was analysed by Western blot using anti-HA antibody.
-
bioRxiv - Genomics 2024Quote: ... Samples were then run on a 2% agarose gel with 1x SYBR Safe for 2 hours at 120 V before gel extraction with a Freeze ‘N Squeeze column (Bio-Rad). After extraction ...
-
bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs) ...
-
bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
-
bioRxiv - Microbiology 2020Quote: ... lysates were incubated with 0.5μg V5-tag antibody (Bio-Rad) and ProteinG sepharose (GenScript ...
-
bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
-
bioRxiv - Microbiology 2022Quote: ... cecorum isolates were inoculated onto Columbia agar supplemented with 5% of sheep blood from Bio-Rad and incubated at 35°C for 24 hours in 5% CO2 ...
-
bioRxiv - Immunology 2024Quote: The following antibodies were used in this study: sheep polyclonal anti-TGN46 (#AHP500G, BioRad, 1:2000), rabbit polyclonal LRBA antibody (#HPA023567 ...
-
bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
-
bioRxiv - Biochemistry 2022Quote: ... stock solution of 40% neutral acrylamide and N,N’-methylenebisacrylamide in a 19:1 ratio (Bio-Rad) were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt ...
-
bioRxiv - Systems Biology 2022Quote: ... The library of cells expressing domains was collected and cell density was counted by flow cytometry (Biorad ZE5). To fix ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Mouse anti-V5-Tag antibody (MCA1360GA, Bio-Rad, Hercules, CA, USA) at 1:2000 dilution was used to detect V5-tagged v-Src ...
-
bioRxiv - Molecular Biology 2020Quote: ... goat anti-human IgG Fc (Biorad; 5211-8004; 1:2000) and rabbit anti-Vinculin (EPR8185 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 4 hpf (n=40) or 48 hpf (n=25) embryos using the Aurum Total RNA Mini Kit (Bio-Rad). cDNA was then prepared with the RevertAid reverse transcriptase (Thermo Fisher) ...
-
bioRxiv - Immunology 2019Quote: ... was detected with goat anti-rabbit AF 647 and sheep anti-TGN46 primary antibody (Biorad, AHP500, 1:100) was detected with donkey anti-goat AF 647 (1:500).
-
bioRxiv - Microbiology 2021Quote: ... Western blots were performed using either mouse anti-V5 tag (Bio-Rad), 746-868 rabbit poly clonal IgG ...
-
bioRxiv - Biophysics 2023Quote: ... extracted agarose gel parts were divided by cutting several times and purified via Freeze N′ Squeeze columns (Freeze N′ Squeeze, 7326165, BioRad) according to the manufacturer’s instructions using a benchtop centrifuge (Biofuge fresco ...
-
bioRxiv - Immunology 2023Quote: ... mice receive daily intradermal injections of CSF1-FC (Bio-Rad; PPP031) or PBS in contralateral hind paws (5µl per paw ...
-
bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
-
bioRxiv - Biophysics 2019Quote: ... N-methylene-bis-acrylamide (Bio-Rad, Herculers, USA) are added ...
-
bioRxiv - Developmental Biology 2020Quote: Antibodies used in this study are specific to sheep GFP (1:500; AbD Serotec, Bio-Rad, Hercules, CA, USA), chick GFP (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA from HEK293 cells were separated on 5% Criterion™ TBE polyacrylamide gels (#3450048, Bio-Rad) and transferred to positively charged nylon membrane ...
-
bioRxiv - Genetics 2021Quote: ... to detect endogenous RPA1 or anti-V5 tag antibodies (mouse, BioRad; 1:100) to detect ectopically expressed RPA1 ...
-
bioRxiv - Microbiology 2022Quote: ... The relative abundance of Spike transcripts was quantified by quantitative real-time PCR (qPCR) using CFX384 machine (BioRad) with a fluorescence reporter (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... and resuspended in FACS buffer with Fc-block (Human Seroblock, Bio-Rad). These samples were then re-pelleted and suspended in a cocktail of fluorophore-conjugated primary antibodies (Supp ...
-
bioRxiv - Physiology 2024Quote: ... Fc-receptors were then blocked for 15 minutes using FcBlock (BioRad – BUF041A). Cells were pelleted by centrifugation (300 x g,5 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Fc-receptors were then blocked for 15 minutes using FcBlock (BioRad – BUF041A). Cells were pelleted by centrifugation (300 x g ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times and incubated with secondary antibodies: Donkey anti Sheep/Goat IgG (dilution 1:100,000, #STAR88P, Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
-
bioRxiv - Bioengineering 2019Quote: ... by means of O/N wet transfer (Bio-Rad) 4 °C at 30 V ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 μl of sequin spike (https://www.sequinstandards.com) was added to 14 μl of RNA for cDNA synthesis with SsoAdvanced iScript (Bio-Rad), followed by RT-qPCR using SsoAdvanced master mix (Bio-Rad) ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates for quantitative Spike western blotting were loaded onto TGX Stain-Free 4-20% gradient gels (Bio-Rad, 4568094) (Fig ...
-
bioRxiv - Microbiology 2022Quote: ... Bound IgG1 and IgG2 antibodies were detected by adding 100μl/well sheep anti-bovine IgG1 or IgG2 HRP (Bio-Rad, USA) at 1:5000 and 1:2500 respectively.
-
bioRxiv - Biochemistry 2022Quote: ... 1:10 000 dilution for N-terminal Parkin Ab) was incubated one hour at RT and protein visualized using ClarityTM Western ECL Substrate (Bio-Rad).
-
bioRxiv - Biophysics 2022Quote: ... Mouse anti-tubulin b3 antibody which recognizes the C-terminal tail of β tubulin was purchased from Bio-Rad Laboratories.
-
bioRxiv - Microbiology 2024Quote: Terminal serum samples were tested in duplicate for cytokine and chemokine levels on a Bio-plex 200 (Bio-Rad) using a Bio-plex Pro Mouse Cytokine 23-plex assay according to kit instructions ...
-
bioRxiv - Microbiology 2022Quote: ... anti-Metapneumovirus N mouse monoclonal (1:25) (MCA4674, Bio-Rad), or anti-Influenza A nucleoprotein (NP ...
-
bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
-
bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
-
bioRxiv - Cancer Biology 2023Quote: ... KM12 or HCT116 cells (10 million) along with the spike-in control (20%) were carefully resuspended in cell suspension buffer (BioRad). The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit) ...
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
-
bioRxiv - Microbiology 2023Quote: HIV-1/N and M strains were quantified by targeting the long terminal repeat (LTR) region using GENERIC HIV Charge virale kit (Biocentric) performed on CFX96 Deep-Well (Biorad) with the followed program RT 10 min at 50°C and 5 min at 95°C ...
-
bioRxiv - Biophysics 2023Quote: ... RBD-Fc then purified by gravity flow using Econo-Glass column (BioRad, California, USA). RBD-Fc elution was performed using 0.1 M glycine (pH2.2 ...
-
bioRxiv - Microbiology 2024Quote: ... then visualized on the Odyssey Fc (LICOR) using Clarity Western ECL Substrate (Bio-Rad).
-
bioRxiv - Microbiology 2022Quote: ... Marcy l’Etoile, France), sheep blood agar with colistin and nalidixic acid (CNA, bioMérieux) and chromogenic agar (Uriselect 4, Bio-Rad, Marnes-la-Coquette, France). Bacterial growth was determined after 18 h of incubation at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... was purchased from G-Biosciences and Freeze ‘N Squeeze DNA gel extraction columns by Bio-rad, Inc ...