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Citations for Bio-Rad :
501 - 550 of 1532 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein was purified by chitin-based affinity chromatography upon elution with 50 mm DTT to cleave the intein tag and further by gel permeation chromatography on a Superdex S200 column using the NGC liquid chromatography system (Bio-Rad). ZmCKX5 (23 mg mL-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... (2) post-AP beads were washed twice in 500μl 2% SDS wash buffer (2% v/v SDS (BioRad, #1610418), 150mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Peroxidase activity was visualized by the enhanced chemiluminescence detection system (Interchim) using the ChemiDocTM MP imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... The blots were developed with a peroxidase-labeled goat anti-mouse or anti-rabbit secondary antibody (1:5,000, Biorad) and chemiluminiscence detection (MF-ChemiBIS 3.2 ...
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit or anti-mouse IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1 h at room temperature with peroxidase-conjugated anti-mouse (1:3000; BioRad, Hercules, CA) or anti-rabbit (1:3000 ...
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bioRxiv - Cell Biology 2021Quote: ... signals were obtained with peroxidase-conjugated secondary antibodies (Cell Signalling Technology) and revealed with Clarity Western ECL kit (Biorad) and a ChemiDoc ™ XRS Imaging system (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... The membranes were washed in TBS-Tween and incubated with the peroxidase-conjugated secondary antibody (Bio-Rad, Table 1) for 1 hour at RT ...
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bioRxiv - Genomics 2022Quote: ... membranes were rinsed threetimes in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Cell Biology 2023Quote: ... endogenous peroxidase activity was blocked in 3.5% (v/v) hydrogen peroxide and blocking with 10% donkey serum (Bio-Rad) was performed ...
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bioRxiv - Cell Biology 2023Quote: ... endogenous peroxidase activity was blocked in 3.5% (v/v) hydrogen peroxide and blocking with 10% donkey serum (Bio-Rad) was performed ...
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bioRxiv - Microbiology 2023Quote: ... membranes were developed in a solution of equal volumes of ECL peroxidase and luminol reagents (Bio-Rad 170-5060).
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011 and 1:5,000 dilution, ref: 170-6515, Bio-Rad) in blocking buffer ...
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bioRxiv - Immunology 2019Quote: ... After quantifying the optimal virus-to-erythrocyte concentration (4HA units), serial two-fold dilutions of Fc, control IVIG (GammaGard, Baxter Healthcare) and polyclonal goat anti-influenza B (Biorad) were prepared ...
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bioRxiv - Plant Biology 2021Quote: ... plants were irradiated with UV-B lamps using fixtures mounted 30 cm above the plants (2 W m−2 UV-B and 0.6 W m−2 UV-A, Bio-Rad ChemiDoc™XRS UV-B lamps ...
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bioRxiv - Microbiology 2020Quote: ... Immunopurified proteins were also stained with Pro-Q® Diamond phosphoprotein gel dye (Thermo) or Phospho-Tag (ABP Biosciences, MD, USA) and visualized in a ChemiDoc MP imaging system (Bio-Rad systems) under specific emission/excitation wavelengths (550/580 nm).
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and the supernatant incubated during 4 h at 4°C with 50 µl of V5 magnetic beads (20 mg/mL of Dynabeads® M-280 Tosylactivated, Invitrogen, coupled with Anti V5-Tag Antibody, clone SV5-Pk1, Bio-Rad). The unbound fraction was discarded and the beads washed 2 times with 1ml of IP Buffer and additionally twice more with 1 ml ice cold PBS + protease inhibitors ...
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... 2% SDS (Bio-Rad), 10% glycerol (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... 0.128mM 2-Mercaptoethanol (BioRad), and 1X Leukemia Inhibitory Factor (purified and gifted by Barbara Panning Lab at UCSF).
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bioRxiv - Biophysics 2021Quote: ... 2% bis-AA (BioRad) as described elsewhere 72,73 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2% bis-AA (BioRad) as described elsewhere (Fischer et al. ...
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bioRxiv - Genomics 2022Quote: ... 2% SDS (Bio-Rad) and 2 mg/mL proteinase K (New England Biolabs) ...
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bioRxiv - Developmental Biology 2019Quote: ... Bis (2%, Bio-Rad), the sonicated nanorod-water solution (sonicated in in bath sonicator for one hour ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... MOMA-2 (Bio-Rad), CD45.2 (104 ...
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bioRxiv - Biochemistry 2020Quote: ... Biobeads SM-2 (BioRad) were presoaked in methanol ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2- Mercaptoethanol (BioRad) and ran through a 4-20% Mini-ProTEAN TGX gel (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... with 2-mercaptaethanol (BioRad) and boiled at 95 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... using Tetrad 2 (BioRad) following the manufactures protocols ...
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bioRxiv - Cell Biology 2023Quote: ... and 2-Mercaptoethanol (BioRad) at 95°C for 5 minutes.
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5 000, Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were next washed and incubated for 1 hour at room temperature with peroxidase conjugated anti-rabbit (1:5000, BioRad), or anti-mouse (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... membranes were incubated with peroxidase-conjugated secondary antibodies and visualized by enhanced chemiluminescence using Clarity Western ECL substrate (Bio-Rad). Target protein levels were normalized against total protein (as determined by Amido Black staining).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were further incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000) (BioRad, Hercules, CA, USA) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Corresponding mouse or rabbit horseradish peroxidase-conjugated secondary antibodies (diluted 1:10,000 in 1x TBST) were added for 1 hr at room temperature (Bio-Rad). Chemiluminescence was detected by adding Clarity Western ECL Substrate (1:1 of luminol and peroxide solutions ...
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bioRxiv - Immunology 2023Quote: ... IgA and IgG antibody isotypes were detected by horse reddish peroxidase (HRP)-conjugated goat anti-pig antibodies specific for porcine IgA (clone AAI40P, BioRad) diluted 1:5 000 ...
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bioRxiv - Immunology 2023Quote: ... After three washing steps membranes were incubated with respective peroxidase-conjugated secondary antibodies and signals were detected using chemiluminescence with a ChemiDoc MP Imaging System (Biorad). Antibodies and dilutions are listed in Supplementary Table 1.
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with horseradish-peroxidase-linked secondary antibodies diluted 1:5000 followed by exposure to Clarity Western ECL Substrate (Cat. No. 170-5060, BioRad) and film development ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with respective peroxidase-conjugated secondary antibodies and signals were detected using chemiluminescence with a ChemiDoc MP Imaging System (Biorad). Antibodies and dilutions are listed in Supplementary Table 2.
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bioRxiv - Neuroscience 2024Quote: ... Imaging was performed using either an Odyssey Fc imaging system (Li-Cor) (quantified using Image StudioTM software) or a Molecular ChemiDocTM Touch Imaging System (Bio-Rad) (quantified using Image Lab 6.0.1 software).
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed thrice with TBS-T before incubation for one hour at room temperature with secondary antibodies (horse radish peroxidase goat anti-mouse or anti-rabbit; Bio-Rad), all at 1:5000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Cancer Biology 2021Quote: ... the membrane was incubated with an anti-rabbit or anti-mouse IgG antibody conjugated with horse radish peroxidase (HRP) (BioRad Laboratories) and developed with Clarity Western ECL Substrate (BioRad Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen-primary antibodies complexes were detected with secondary antibodies conjugated to horse radish peroxidase and developed using a chemiluminescent reagent (Clarity ECL, Bio-Rad). Images of the WBs were acquired using a ChemiDoc MP System (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... anti-rabbit IgG (H + L) secondary antibody and goat peroxidase-conjugated anti-mouse IgG (H + L) secondary antibody were purchased from Bio-Rad.
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bioRxiv - Pathology 2023Quote: ... Signal was visualized using a secondary horseradish-peroxidase-labeled goat anti-mouse antibody (goat anti-mouse IgG-HRP 1:5000, SantaCruzBiotechnology) and enhanced chemiluminescence (ECL Clarity Bio-Rad). The purity as well as equal loading (40γ ...