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Citations for Bio-Rad :
1 - 50 of 2372 citations for Rh Family C Glycoprotein RHCG Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... Drl family ECRs were further purified using an UnoQ anion exchange column (Bio-Rad), loading in 20 mM HEPES pH 7.5 containing 70 mM NaCl and using an elution gradient of 70 mM-1 M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... a 160kD glycoprotein expressed by murine macrophages 235 (1:100, Bio-Rad clone C1:A3-1, Cat. MCA497) and rabbit anti-laminin (1:50 ...
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bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
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bioRxiv - Neuroscience 2020Quote: ... plates were coated with 100 μl of either 1 μg/ml of rh-TfR1-ECD (purified in-house) or liver purified ferritin (Bio-Rad, 4420-4804) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories, USA) with 2 mM ATP (MP Biomedicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% non-fat dry milk and incubated with primary antibodies overnight at 4 °C and then incubated with host species-matched HRP-conjugated secondary antibodies (BioRad) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were incubated overnight at 4°C with primary antibodies and then probed with secondary antibodies before ECL development and imaging (Bio-Rad). Primary antibodies used for immunoblotting are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C followed by horseradish peroxidase-conjugated secondary IgG antibody (Bio-Rad Laboratories) for 1h at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Cell Biology 2022Quote: ... at 4 °C for overnight followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detection using Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated overnight at 4°C with primary antibodies dissolved in 5% Blotting-Grade Blocker (Bio-Rad). All primary antibodies and dilutions used are listed in Supplementary Table STm.1 ...
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bioRxiv - Biochemistry 2023Quote: ... the flow chamber was incubated at 4°C overnight with 25 ng/µL Digoxigenin Antibody (Bio-Rad) that adsorbed onto the polystyrene-covered lower surface ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 were incubated overnight at 4°C and revealed using peroxidase-conjugated antibodies and an ECL kit (BioRad). Images were captured using ChemiDocTM XRS Imaging system (Biorad).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4970) overnight at 4 °C and the HRP-conjugated secondary antibodies (BIO-RAD, Cat. No. 1706515, 1:1000) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunostaining was performed overnight at 4° C using primary antibodies for PTH (1:400, Bio-Rad, Hercules, CA), Cleaved caspase 3 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
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bioRxiv - Microbiology 2022Quote: ... 59.2°C and 60°C using PCR thermal cycler (Biorad) for 30 min before HA assay.
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bioRxiv - Microbiology 2022Quote: ... 59.2°C and 60°C using PCR thermal cycler (Biorad) for 30 min or left at 4°C as control before HA assay.
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bioRxiv - Biophysics 2022Quote: ... Mouse anti-tubulin b3 antibody which recognizes the C-terminal tail of β tubulin was purchased from Bio-Rad Laboratories.
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Biochemistry 2021Quote: ... Mixtures were heated to 90°C for 10 min and slowly cooled to 40°C (0.1°C / second) using a Dyad PCR machine (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated from 10 °C to 95 °C (10 s at each 0.5 °C step) using a CFX Connect qPCR (Bio-Rad). Melting temperatures were calculated with DSFWorld61 using model 1 in the “by sigmoid fitting” option ...
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bioRxiv - Biochemistry 2022Quote: ... with a three-step protocol (95°C 15 sec, 60°C 30 sec, 68°C 60 sec) and iTaq Universal SYBR Green Supermix (Bio-Rad). Primer sequences are provided in Table 2 ...
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bioRxiv - Physiology 2019Quote: ... paraffin-embedded sections were incubated at 4°C overnight with antibodies for F4/80 (MCA497G, Bio-Rad Laboratories, Hercules, CA, USA), HILPDA (sc-137518 HIG2 Antibody (C-14) ...
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bioRxiv - Neuroscience 2019Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Neuroscience 2021Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Physiology 2020Quote: ... rabbit polyclonal anti-Cytochrome C (Cyt C) (AHP2302, Bio-Rad Laboratories, 1:500), mouse monoclonal anti-MMP2 (sc-13595 ...
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bioRxiv - Bioengineering 2020Quote: ... and annealed from 90°C to 20°C at 1 °C min-1 in a T100™ Thermal Cycler (Bio-Rad, USA). Following construction ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained in different cycles overnight at 4°C with the following antibodies: anti-CD3 (clone CD3-12; #MCA1477, Bio-Rad, USA), anti-CD206 (clone E6T5J ...
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bioRxiv - Developmental Biology 2021Quote: ... denaturation at 95°C by 0.5°C in a Real-Time PCR apparatus (BioRad, USA). The genes of interest in this study are listed in supplementary Table 3 ...
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bioRxiv - Biochemistry 2022Quote: ... from 20°C to 95 °C in C1000 Thermal Cycler CFX96 Real-time System (Bio-Rad). Tm was determined as a local minimum of the first derivative of the raw fluorescence as function of temperature with software Bio-Rad CFX Manager 3.1.
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bioRxiv - Physiology 2023Quote: ... 120 min at 37 °C and 5 min at 85 °C in a Thermocycler (T100, BioRad). For real-time amplification ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at 70°C. The following primary antibodies were used (see also Suppl. Table 1 for details): mouse monoclonal IgG1 anti-βCYA (Bio-Rad, clone 4C2, RRID:AB_2571580), mouse monoclonal IgG2b anti-γCYA (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... “L”represents the Western C (BioRad) protein ladder ...
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bioRxiv - Molecular Biology 2024Quote: ... at 4°C (Bioruptor Pico, BioRad). Then ...
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bioRxiv - Cell Biology 2023Quote: ... 62 cycles (10′′ 65 °C + 0.5 °C) in the SYBR-Green-Cycler IQ5 detection protocol (Biorad CFX384), performed in 384-well plates (Merck) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 4 minutes at 72°C and then final extension at 72°C for 10minutes (MyCycler, Bio-rad). After the reaction ...
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bioRxiv - Immunology 2022Quote: ... 42 °C for 50 min and 85 °C for 5 min in a thermal cycler (Bio-Rad, USA). Synthesized cDNA was kept at −80 °C till further use ...
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bioRxiv - Cell Biology 2023Quote: ... Precision Plus Protein Western C ladder (BioRad) and Precision protein Streptactin-HRP conjugate (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... The temperature was varied 25 - 95 °C in 0.5 °C increments by using an iCycle IQ5 real-time PCR system (BioRad). Relative fluorescence was measured using excitation and emission wavelengths of 580 nm and 623 nm ...