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Citations for Bio-Rad :
1 - 50 of 8675 citations for Retinol Binding Protein RBP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The binding to protein G magnetic beads (Biorad) was performed for 1 hour at 4 ºC (with previous washes with a RIPA buffer) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein binding was detected by using ECL substrate (BioRad) and visualised by ImageQuant4000 (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The protein concentration was measured using the Bradford dye-binding protein assay (Bio-Rad), using known concentrations of bovine serum albumin as external standards.
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration was determined using the Bradford protein dye binding assay (#5000113 + #5000114, Bio-Rad). 50μg of protein was separated by electrophoresis in an SDS-polyacrylamide gel which was then transferred using the semi dry method ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were quantified with the Coomassie dye binding method (BioRad, 500-0006) as described by the manufacturer.
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bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined by the dye-binding method of Bradford assay (Bio-Rad) and bovine serum albumin as the calibration standard ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
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bioRxiv - Cell Biology 2022Quote: ... a DNA-binding fluorescent dye (BioRad, SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared with icecold lysis buffer and the protein concentration was measured by Coomassie binding (Bio-Rad). Equal amounts of protein were added to each well of a G-LISA plate and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: All binding assays were performed on a ProteOn XPR36 SPR Protein Interaction Array System (Bio-Rad Laboratories, Hercules, CA, USA). The instrument temperature was set at 25 °C for all kinetic analyses ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed four times with binding buffer containing 0.1% Tween-20 and bound proteins were eluted with 2X Laemmli Sample Buffer (Bio-Rad Laboratories). Eluted proteins were subjected to electrophoresis on a 17% resolving gel at 120V for 2 hrs and transferred to PVDF membrane at 180mA for 2 hrs and immunoblotted with His-Probe-HRP (Pierce ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Molecular Biology 2020Quote: ... using a DNA-binding fluorescent dye (BioRad, SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using chemiluminescence (BioRad) on a BioRad ChemiDocTM Imaging System.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and stored at -80 until α5 subunit and its binding partners were immunoprecipitated using SureBeads protein G magnetic beads according to the manufacturer’s protocol (Biorad, Hercules, CA, USA). Western blots (detailed in section 2.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent, Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Biochemistry 2022Quote: Equilibrium target binding assays were performed using a double-membrane filter-binding setup with a slot blot vacuum filtration device (BioRad SF) containing an upper nitrocellulose membrane (to capture protein and protein/RNA complexes ...
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bioRxiv - Plant Biology 2021Quote: ... Protein concentrations were quantified using the Coomassie Blue dye binding method (Bradford, 1976) using a concentrated dye purchased from BioRad (500-0006, Hercules, CA, USA). AsA and DHA concentrations were determined by an ascorbate oxidase based method according to Lin et al ...
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bioRxiv - Microbiology 2021Quote: ... binding was visualized with an enhanced chemiluminescence substrate (Bio-Rad) using the Bio-Rad Gel Doc XR+ System and Image Lab software according to the manufacturer’s instructions (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected by chemiluminescence(Clarity kit, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected by chemiluminescence (Clarity kit, Bio-Rad).
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bioRxiv - Systems Biology 2020Quote: ... unspecific binding was blocked using PBS/10% AB-serum (Bio-Rad) for 30 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody binding was processed for ECL detection (Bio-Rad) with appropriate HRP-conjugated secondary antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... Antibody binding was visualised using Clarity Western ECL Substrate (Biorad, UK) and viewed under the Gel Doc imaging system (GeneSys software ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked using PBS/10% AB-serum (Bio-Rad, Germany). Alkaline phosphatase was developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitroblue tetrazolium (BCIP/NBT ...
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bioRxiv - Systems Biology 2020Quote: ... Unspecific antibody binding was blocked with PBS/10% AB-serum (Bio-Rad) for 5 min at 4°C prior to staining with CD19 or CD3 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody binding was detected using an ECL chemiluminescence kit (Bio-Rad, 1705061). The following antibodies were used ...
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bioRxiv - Immunology 2023Quote: ... Binding was detected with an anti-mouse IgG-HRP (STAR120P, Bio-Rad). ELISAs were developed using 1-Step-Ultra TMB ELISA substrate (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Binding was measured using a Bio-Plex 200 instrument (Bio-Rad Laboratories, Inc.). HIV-1 human hyperimmune immunoglobulin (HIVIG ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific binding sites were blocked by 5% non-fat dry milk (Bio-Rad Laboratories) in Tris buffered saline (TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were resuspended in 20µL binding buffer and 10µL 4X Laemmli sample buffer (Biorad) supplemented with b-mercaptoethanol ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific binding was blocked for 1 h at room temperature with 5% blotto (Biorad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Microbiology 2021Quote: ... The antibody binding was imaged with Clarity Western ECL Substrate (Bio-Rad, CA, USA).
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bioRxiv - Genetics 2020Quote: ... The binding reactions were resolved in 5% nondenaturing TBE pre-cast gel (Bio-rad) using Mini-PROTEAN® Electrophoresis System (Bio-rad ...
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bioRxiv - Cell Biology 2024Quote: ... The antibody binding was assessed with a ChemiDoc Imaging System (Bio-Rad, Madrid, Spain) and the density of bands was measured using ImageJ software v.1.8.0_172 (NIH ...
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bioRxiv - Neuroscience 2020Quote: ... Aspecific binding sites were blocked for 30 min using TBS + 1% casein (BioRad, Temse, Belgium). Western blot was performed using the following primary antibodies overnight (4°C) ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked by using PBS with 5% human AB serum (Bio-Rad, Germany) and 5% mouse serum (Agilent ...
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bioRxiv - Immunology 2020Quote: ... Binding of mAb to glycan was determined with A Bio-Plex 200 HTS (Bio-Rad) machine with the Bioplex manager software (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Specific binding was detected by enhanced chemoluminescence using Clarity Western ECL Substrate (BioRad, Hercules, CA) and visualized with a FUSION SOLO 4M system (Vilber Lourmat ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding sites were blocked with 5% fat-free dry milk (Bio-Rad Hercules, California) in PBS containing 0.1% Tween-20 (PBST ...
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bioRxiv - Cancer Biology 2019Quote: ... and the binding of HRP-conjugated antibodies was visualized using Clarity Western ECL Substrate (Bio-Rad). For immunoblotting we used the following antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Single antigen-binding B cells were sorted using an S3 cell sorter (Bio-Rad, Hercules, CA), and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio ...