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Citations for Bio-Rad :
301 - 350 of 359 citations for Recombinant Rat Il1b His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and stained with rat anti-mouse CD68 primary antibody (clone FA-11; Bio-Rad, Hercules, CA) followed by biotinylated rabbit anti-rat IgG secondary antibody (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
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bioRxiv - Biochemistry 2022Quote: ... acidified to pH3–4 by adding appropriate amount of TFA and subjected to HPLC purification using a C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad, Hercules, CA, USA). The S-sulfonated FAM237A was eluted from the C4 reverse-phase column by an acidic acetonitrile gradient composed of the acidic solvent A (0.1% aqueous TFA ...
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bioRxiv - Biochemistry 2023Quote: ... and the S-sulfonated FAM237B was eluted from a C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad, Hercules, CA, USA) by an acidic acetonitrile gradient ...
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bioRxiv - Microbiology 2024Quote: ... and HopAM1 and C-terminal Flag-tagged IpaH4 were generated by PCR amplification using primers containing Flag sequences and flanking SacII / PacI off pIB/V5-His templates using iProof DNA polymerase (Bio-Rad; Table S4) and cloned into the pDGOpIE2 vector ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Immunology 2021Quote: ... Recombinant Bovine Interleukin-8 PBP039 and Goat anti Bovine Interleukin-8 Ab conjugated to biotin AHP2817B (all from Bio-Rad, protocol according to the manufacturer’s intructions).
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Biophysics 2023Quote: The monovalent streptavidin sample was kindly provided by the Howarth Lab (University of Oxford),23 and recombinant IgE Kappa (clone AbD18705_IgE, Cat#HCA190) was purchased from Bio-Rad Laboratories.
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
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bioRxiv - Neuroscience 2020Quote: ... Germany) and lysosomes with rat monoclonal anti-Cd68 (clone FA-11, #MCA1957, Bio-Rad, Hercules, CA, USA). In the second set ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Neuroscience 2022Quote: ... was used for quantitative real time PCR with commercially available rat Magel2 and Gapdh primers (Bio-Rad, USA ...
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bioRxiv - Neuroscience 2024Quote: ... For analysis the Bio-Flex Pro™ Rat Cytokines Assay (Bio-Rad, 24-plex panel #171-K1001M) was used on a Bio-Plex® MAGPRIX™ multiplex reader and analysed with Bio-Plex Analysis software (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: SDS-PAGE analysis of the recombinant TMPT2A (rTMPT2A) was performed on linear 12.5% polyacrylamide minigels in a Mini-Protean® Tetra cell system (BioRad, USA), as described by [22] ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... and the fractions containing the recombinant vaccine candidates were automatically collected using BioFrac™ Fraction Collector (Bio-Rad Laboratories, Hercules, USA) when the 280 nm absorbance was greater than 0.05 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were stained with rat monoclonal anti-BrdU [clone BU1/75 (ICR1)] (BioRad Cat #MCA 2060, RRID: AB_323427), rabbit monoclonal anti-active Caspase-3 (clone C92-605 ...
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bioRxiv - Immunology 2019Quote: ... Rosa26LSL-tdTomato/+ animals (6-12 weeks) were given 1 μg of FITC-conjugated rat anti-CD169 antibody (BioRad) diluted in a total volume of 20 μl of PBS into the right footpad to label CD169+ subscapular macrophages inside the draining LN ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-rat (1:1000; Alexa Fluor 568, #A11077) and V5-tag:AlexaFluor-647 (1:200; Bio-Rad MCA1360A647).
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bioRxiv - Cancer Biology 2020Quote: 10 μm sections of mouse tumors were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), and goat anti-human TSP-4 (AF2390 ...
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bioRxiv - Physiology 2021Quote: Quantitative PCR for rat FXR and reference gene GAPDH was performed using SsoAdvanced Unviersal SYBR Green Supermix (BioRad) in the ViiA 7 Real-Team PCR System (Thermo Fisher ...
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bioRxiv - Physiology 2021Quote: ... incubated with primary antibody (monoclonal rat anti-F4/80, 1:200 dilution, MCA497; BIO-RAD; Hercules, CA, USA) for one hour ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin YL1/2 from rat (MCA77G, Bio-Rad AbD Serotec, at 1:2000 dilution for IF), anti-rabbit Alexa Fluor 488 (A11008 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Neuroscience 2023Quote: ... Axonal staining and myelination in the SLM were assessed with rat anti MBP (BIO-RAD, cat # MCA409S 1:500) and mouse anti-Neurofilament H (NF-H) ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-IBA-1 antibody (0.4 μg/mL, FUJIFILM Wako) and rat anti-CD68 antibody (5 μg/mL, clone FA-11, Bio-Rad) was used ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were blocked with 3% donkey serum and incubated overnight with rat anti-CD68 (1:200, Bio-Rad). Aβ deposition and CD68 staining were both developed with a Vectastain ABC kit and DAB reaction ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody cocktail against CD68 (rat monoclonal; 1:250; catalog no. MCA1957; Bio-rad), GFAP (Chicken polyclonal ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: The recombinant protein of each vaccine candidate was purified under denaturing conditions using a biologic duoflow™ chromatography system (Bio-Rad Laboratories, Hercules, USA). A HisTrap™ HP column (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were used for flow cytometric analysis after cells were stained with Alexa647-conjugated rat anti-mouse CD204 antibody (clone 2F8; Bio-Rad Laboratories GmbH ...
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Pathology 2021Quote: ... The sections were then incubated in blocking buffer (10% goat serum; 1 hour) followed by incubation with either rat anti-F4/80 antibody (Bio-Rad) diluted 1:500 or rat anti-CD68 antibody (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression levels of 85 genes were assessed using the PrimePCR Antibacterial Response (SAB Target List) R384 rat plate (Catalog #10047065; Bio-Rad) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... tissue sections were blocked in 10% normal goat serum for 1 h at room temperature and incubated in rat anti-mouse CD4 (1:200; MCA2691; Bio-Rad) or CD8a (Biolegend ...