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Citations for Bio-Rad :
51 - 100 of 9607 citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... His6-tagged proteins were then captured from the lysate by Ni2+-IDA chromatography (Profinity IMAC resin, Bio-Rad) under gravity flow and washed with 35mM Imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Biotinylated proteins representing the total and surface pools were separated by SDS-PAGE (4-15% Tris/glycine, Biorad) and blotted for the γ subunit using anti-HA-Peroxidase antibodies (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... the biotinylated proteins were eluted from the beads by incubation with Laemmli sample buffer supplemented with BME (BioRad) and 2 mM biotin at 90°C for 10 min.
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bioRxiv - Cancer Biology 2023Quote: ... Depletion of biotinylated proteins from lysates was confirmed by immunoblotting using Streptavidin HRP antibody (STAR 5B, Bio-rad).
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Immunology 2023Quote: ... SIgA was detected by HRP-conjugated streptavidin using biotinylated mouse anti-pig secretory component (clone K60 1F1, BioRad) diluted 1:2 000 ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates or recombinant protein were loaded on a 4-20% Criterion gel (BioRad, 3450032) for SDS-PAGE and transferred to PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant proteins were affinity-purified from cleared lysates using a NGC Quest Plus FPLC system (Biorad) and GSTrap HP (GST-tagged BmVreteno and GST) ...
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bioRxiv - Immunology 2023Quote: The biotinylated ODN-bound proteins were precipitated with streptavidin beads from mitochondria lysates and resolved by PAGE (Bio-rad). The PAGE gels were stained using a silver staining kit (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and antimouse secondary antibody (Biorad; 1:10,000). GroEL was used as cytoplasmic control and detected as above ...
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bioRxiv - Plant Biology 2024Quote: ... in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad) and the ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The gel carrying the recombinant proteins and their derivatives was eventually stained with Coomassie Blue (Bio-Rad) for 1 hour and was de-stained with a de-staining solution (20% methanol and 10% acetic acid in water).
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bioRxiv - Neuroscience 2023Quote: ... mouse anti Human Protein Gene Product 9.5 (PGP9.5) (1:500, MCA4750GA mouse monoclonal, Biorad) for 36 h at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Bioengineering 2019Quote: Equal volumes of (GT)6-Cy5-SWCNT and FAM-tagged protein at 2X working concentration were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescently tagged/labeled expressed protein was detected both before and after SDS-PAGE using a Chemidoc MP imaging system (Bio-Rad, Laboratories Pty ...
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bioRxiv - Microbiology 2023Quote: ... 25 µg or 50 µg of normalized protein for LapA-HA or MapA-HA tagged strains respectively was mixed with 4x Laemmli Sample Buffer (Bio-Rad) and 2-mercaptoethanol to a 1x working dilution and samples were boiled for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein purities were assessed by SDS-PAGE using 4-20% Mini-Protean TGX gels (Bio-Rad, #4561094) and quantified using the BCA Protein Estimation kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... by immunoprecipitation of endogenous or endogenously Flag-tagged Piwi (eF-Piwi): The anti-Piwi antibody and Surebeads Protein A Magnetic bead (Bio-Rad, 1614013) were used to immunoprecipitated endogenous Piwi-piRNAs from witld type OSC ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Plant Biology 2019Quote: ... The mixed beads were washed once with the washing buffer and biotinylated proteins were eluted by 30 μL 2× SDS sample buffer and separated by SDS-PAGE (Biorad, Hercules, CA).
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bioRxiv - Microbiology 2021Quote: ... were treated with 20% blocking buffer (Li-Cor Biosciences, Lincoln, US) followed by incubation with anti-His-tag mouse primary antibody (dilution1/5000, Bio-Rad) and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000 ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated with a secondary goat anti-rabbit (for the anti-YjbI antiserum) or goat anti–mouse (for the anti–His-tag antibody) antibody conjugated with horseradish peroxidase (Bio-Rad) for 2 h ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-tagged secondary antibodies (Bio-Rad, 1721011 and 1721019) were used in combination with Super-Signal ECL kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Rhodamine-tagged anti-actin (Bio-Rad #12004163; 1:10,000) was used as a loading control ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... for specific tardigrade proteins and anti-mouse Starbright 520 (12005867, Bio-Rad) for alpha-tubulin detection ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
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bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and biotinylated anti-phosphotyrosine secondary antibody (BioRad; #MCA2472B; 1:2000) was added followed by ON incubation at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and biotinylated anti-RSV antibody (goat polyclonal IgG, Bio-Rad) was added for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... using biotinylated anti-RSV antibody (goat polyclonal IgG, Bio-Rad) for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... The β-tubulin antibody was tagged with Rhodamine (Bio-Rad #12004166) and was imaged using Rhodamine channel in Chemidoc-MP as per manufacturer’s instruction.
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged LC8 was purified using Ni Profinity IMAC Resin (BioRad) followed by anion-exchange chromatography on HiTrap Q-HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: The recombinant protein of each vaccine candidate was purified under denaturing conditions using a biologic duoflow™ chromatography system (Bio-Rad Laboratories, Hercules, USA). A HisTrap™ HP column (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2022Quote: ... The HN protein was detected by a mouse monoclonal antibody 8H2 (MCA2822, Bio-Rad). The membranes were then washed with PBST on a shaker 3 times (10 min at RT each time ...