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Citations for Bio-Rad :
251 - 300 of 3491 citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
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bioRxiv - Microbiology 2019Quote: ... both with horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (1:10,000; Bio-Rad). Anti-OmpA antibody54 was used at 1:20,000 and a custom anti-TssL antibody (Eurogentec ...
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bioRxiv - Immunology 2020Quote: ... A 1:2000 dilution of isotype specific goat anti-mouse horseradish peroxidase conjugate (Biorad) in PBS supplemented with 2 % (w/v ...
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bioRxiv - Physiology 2020Quote: ... mouse monoclonal anti-Apolipoprotein A1 (Apo-A1) (0650-0050, Bio-Rad Laboratories, 1:200). Membranes were washed 3 times with TBST and incubated with anti-mouse IgG secondary antibody (A16017 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 1:200 or a mouse IgG isotype control (BioRad, Cat.#1706516; RRID: AB_11125547) at 1:200 in 0.75% Saponin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies against CD68 (1:500, mouse-anti-rat, Bio-Rad/Serotec, MCA341R) and Iba1 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... 1:3,000 and secondary antibody goat anti-mouse IgG StarBright Blue 700 (Bio-Rad) 1:2500 ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-mouse IgG pAb conjugated to horseradish peroxidase (1:10,000, 170-6516, Bio-Rad) or anti-rabbit IgG pAb conjugated alkaline phosphatase (1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating simultaneously with Goat Anti-Mouse IgG StarBright Blue 700 (1:1000; BioRad) and Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... goat anti-mouse IgG (H+L)-HRP conjugate (1:5,000 dilution, Bio-Rad Laboratories). After three washes with TBS-T ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad; 1:2000) and goat anti-rabbit IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween-20 (TBS-T): Goat anti-mouse Starbright700 (1:10000, Bio-Rad,12004158), Goat anti-rabbit IRDye800 (1:10000 ...
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bioRxiv - Biochemistry 2021Quote: ... and monoclonal anti mouse secondary antibodies conjugated to horseradish peroxidase (1:5000, Bio-Rad). After incubation with ECL substrate (BioRad ...
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bioRxiv - Genetics 2020Quote: ... membranes were incubated with HRP-conjugated goat-anti-mouse IgG (1:2000; Bio-Rad) for 1 h at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... Secondary antibody was goat anti-mouse IgG horseradish peroxidase conjugate (1:5,000; Bio-Rad). Blots were developed using the Luminata Forte Western HRP substrate reagent (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... either HRP-conjugated goat anti-rabbit or an anti-mouse antibody (1:5,000, BioRad). Antibodies were detected using the enhanced chemiluminescence system (Immobilon Forte Western HRP substrate ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Ab (mAb)-anti-sialoadhesin (Bio-rad, Inc., Hercules, CA, USA; 1:250), goat pAb anti-integrin-α3 (Santa Cruz biotechnology ...
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bioRxiv - Systems Biology 2022Quote: ... StarBright Blue 700 goat anti-mouse IgG (catalog number 12004159; Bio-Rad; 1:10,000), rabbit anti-goat immunoglobulins/HRP (catalog number P0449 ...
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bioRxiv - Biochemistry 2023Quote: ... and visualized using secondary anti-mouse HRP (dilution 1 to 3000, #1706516, Bio-Rad) or anti-rabbit HRP-antibodies (dilution 1 to 3000 ...
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bioRxiv - Cell Biology 2023Quote: ... and HRP-conjugated donkey anti-mouse IgG (1:5000) (Bio-Rad, catalogue number 1706516).
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated goat anti- mouse IgG secondary antibody at 1:10,000 dilution (Biorad, STAR207P).
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bioRxiv - Microbiology 2023Quote: ... either goat anti-mouse coupled with DyLight®800 (Bio-Rad, 1:10000 dilution) or goat anti-rabbit coupled with StarBright Blue 700 (Bio-rad ...
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bioRxiv - Plant Biology 2024Quote: ... The goat anti-mouse IgG-HRP conjugate (cat#1706516, 1:4,000 dilution; BIO-RAD) and the secondary antibody were used to detect anti-GFP and anti-ACTIN.
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bioRxiv - Cell Biology 2023Quote: ... and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516, Biorad, 1:10000). Antibody binding was detected by the enhanced chemiluminescence system (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated with 1:5000 HPRT-conjugated anti-mouse secondary antibody (Biorad, 1706516) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... followed by horseradish peroxidase-conjugated Goat- anti-mouse second antibodies (1:10000; BioRad # 1721011) for 1hr at the room temperature.
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bioRxiv - Developmental Biology 2023Quote: ... and secondary antibodies HRP-conjugated goat anti-mouse IgG (1:5000; 1706516, Bio-Rad) or goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Genetics 2024Quote: ... goat anti-mouse IgG (H+L) HRP conjugate (1:10000, Bio-Rad Cat. # 1706516).
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated secondary antibodies were diluted 1:10000 and 1:2000 for polyclonal Goat-α-Mouse (Bio-Rad, 170-6516) and Goat-α-Rabbit HRP (Dako ...
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bioRxiv - Pathology 2019Quote: ... After incubation of the blots with HRP-conjugated secondary antibodies (1:3000 for goat anti-rabbit or 1:10,000 for goat anti-mouse, Bio-Rad), specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for immunoblotting: Talin-1 (1:5000 dilution, Mouse monoclonal, Clone 97H6; Bio-Rad, Cat# MCA4770), YAP (1:1000 dilution ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies (Anti-Rabbit or Anti-Mouse, CST, 1:5000 and 1:2000) and revealed using Clarity Western ECL (BioRad). Image J was used to quantify the intensity of western blot bands ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Plant Biology 2024Quote: ... in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad) and the ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with fluorescent tagged secondary antibodies for 45 minutes at RT followed by imaging on a fluorescence scanner imaging system (Biorad).
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bioRxiv - Biochemistry 2022Quote: ... and eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Cancer Biology 2022Quote: ... CD206 was detected using rat anti-mouse CD206 (dilution 1/150, Bio-Rad, cat. MCA2235GA) and Alexa Fluor® 546 goat anti-rat antibody (dilution 1/250 ...
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bioRxiv - Molecular Biology 2020Quote: ... We used horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Biorad, 1:2,000). Blots were developed using an enhanced chemiluminescence kit (Amersham ...
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bioRxiv - Cell Biology 2021Quote: ... StarBright® B700 labeled goat anti-mouse IgG secondary antibody (Bio-Rad, 12004158, 1:5000), HRP conjugated goat anti-mouse IgG (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... and secondary antibody Goat Anti-Mouse (H+L) HRP Conjugate (1:250; BioRad #170-6516). The samples were then incubated with peroxidase (HRP ...
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit or anti-mouse IgG conjugated to horseradish peroxidase (Bio-Rad, 1:5,000 dilution) were used as secondary antibody and detected using the Clarity Western ECL Substrate as described by the manufacturer (Bio-Rad) ...
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bioRxiv - Bioengineering 2019Quote: ... A 1:5000 dilution of the “Immun-Star Goat Anti-mouse-HRP conjugate” (1705047, Biorad) was used as secondary antibody under the same conditions ...
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bioRxiv - Cell Biology 2021Quote: ... Goat anti-mouse IgG (H/L):HRP (1:1,000; multi species absorbed; Bio-Rad, STAR117P) and ECL western blot detection reagents (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Bio-Rad, 1:10000) were visualized using ECL Plus Western Blotting Substrate (Thermo Fisher Scientific ...