Labshake search
Citations for Bio-Rad :
101 - 150 of 1105 citations for Recombinant Mouse CD40 Antigen His tagged and Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2021Quote: ... Quantitative analyses of blotted hCDKL5 and His-Neuropinilin were carried out with Image Lab software (BioRad) and the volumetric yield was derived considering the final biomass concentration (OD600 ...
-
bioRxiv - Microbiology 2019Quote: ... The antigen-antibody reaction was analyzed by using Opti-4CN detection kit according to the instructions of the manufacturer (Biorad).
-
bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... and affinity purified anti-HSP-90 antibodies by use of an MBP-HSP-90 antigen coupled to Affigel matrix (BioRad), using a procedure previously published (Benian et al. ...
-
bioRxiv - Molecular Biology 2022Quote: ... The BRM antigen was affinity-purified by IMAC using the NGC Medium-Pressure Liquid Chromatography System (Bio-Rad, Hercules, CA) and eluted in elution buffer (50mM sodium phosphate pH 8.0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... IL-12 (10 ng/mL)] or transferred to a high-binding 96-well plate pre-coated with indicated amounts of control or TAG72 antigen (BioRad). Reagents and buffers for flow cytometry processing were pre-chilled on ice unless otherwise stated ...
-
bioRxiv - Biophysics 2023Quote: ... purified RBD-Fc proteins were concentrated using 10,000 MWCO Amicon centrifugation columns (BioRad, California, USA) and stored at 4°C before use ...
-
bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
-
bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
-
bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Final recombinant protein concentrations were determined using Quick Start Bradford Protein Assays (Bio-Rad) with bovine serum albumin as the reference protein for standard curves ...
-
bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
-
bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
-
bioRxiv - Epidemiology 2019Quote: ... Bloodspot dilutions were assayed in duplicate with antigen-coupled microsphere beads using a BioPlex 200 system platform (Bio-Rad, Hercules, CA) as previously described [21,23,25] ...
-
bioRxiv - Biochemistry 2021Quote: ... The antigen-antibody complexes were visualized using appropriate HRP conjugated secondary antibodies and enhanced chemiluminescence kit (ECL; Amersham or Bio-Rad) and normalization was reported to β-actin ...
-
bioRxiv - Immunology 2023Quote: ... Labeled glycans were dissolved in HPLC grade water (FisherScientific) and excess unbound APTS removed using Agencourt CleanSEQ beads (non-antigen specific glycans) and Bio-Gel P-2 (Bio-rad) size exclusion resin (RBD-specific glycans) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
-
bioRxiv - Neuroscience 2023Quote: ... The antigen–antibody complexes were detected with SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoScientific) using the ChemiDoc XRS+ Imager System (Bio-Rad). Blots were exposed for 3 seconds ...
-
bioRxiv - Molecular Biology 2023Quote: ... The HURP antibody was then purified by first conjugating 10 mg of HURP(462-576) antigen to 0.5 mL of Affi-gel 15 (Bio-Rad: 1536051) resin (brought up to 10 mL with PBS buffer) ...
-
bioRxiv - Microbiology 2023Quote: ... Distinct MagPlex microsphere sets (Luminex Corp, Austin, TX, USA) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2020Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral or control antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Initial screening for IgG antibodies was undertaken using an extensive panel of bacterial (Leptospira, Brucella) and viral antigens (paramyxovirus, filovirus, coronavirus, flavivirus, alphavirus) coupled to MagPlex beads (Bio-Rad, Australia) for multiplex screening ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
-
bioRxiv - Immunology 2023Quote: ... Each antigen concentration was determined by UV absorbance at 450 nm in a plate reader (iMark™ Microplate Absorbance Reader, BioRad, Japan).
-
bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed as previously mentioned and the antigen-antibody complex was revealed by Clarity Enhanced Chemiluminescence Blotting Substrate (Bio-Rad, #1705060) using the iBright™ FL100 Imaging System (ThermoFisher) ...
-
bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
-
bioRxiv - Biochemistry 2022Quote: ... and eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
-
bioRxiv - Cancer Biology 2019Quote: ... a bottom layer containing RPMI 1640 medium (10% CS-FCS) and 0.75% low melting agar (Bio-Rad) was overlain with half the volume of RPMI 1640 medium (10% FCS ...
-
bioRxiv - Cell Biology 2022Quote: ... the cell lysates or recombinant protein were loaded on a 4-20% Criterion gel (BioRad, 3450032) for SDS-PAGE and transferred to PVDF membrane (Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... Recombinant proteins were affinity-purified from cleared lysates using a NGC Quest Plus FPLC system (Biorad) and GSTrap HP (GST-tagged BmVreteno and GST) ...
-
bioRxiv - Immunology 2023Quote: ... Both sets of recombinant proteins were quantified using the Bradford protein quantification assay (Bio-Rad, UK).
-
bioRxiv - Neuroscience 2023Quote: ... mouse anti-V5-Tag:DyLight-550 mouse (1:400, Bio-Rad), chicken anti-GFP (1:400 ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes received a final wash in PBST and the antigen-antibody complex was detected by enhanced chemiluminescence using a ChemiDoc MP Imaging system (Bio-Rad Laboratories, Hercules, CA). The bands in the experimental samples were background subtracted and quantified by mean optical density using computer-assisted densitometry with Image Lab software (v4.0.1 ...
-
bioRxiv - Neuroscience 2022Quote: ... Solid phase specificity was confirmed with dot blots of serial antigen dilutions (2 μL) applied to nitrocellulose membrane (Bio-Rad 0.45 µm; Fig. 1b). Membranes were allowed to air dry (1 h ...
-
bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
-
bioRxiv - Biochemistry 2022Quote: ... The refolded mature FAM237A was eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
-
bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
-
bioRxiv - Microbiology 2023Quote: ... Ni-NTA agarose with the bound His-SUMO-SigN was loaded on a Poly-Prep® Chromatography Column (Bio-Rad), washed with P2 buffer and subsequently with the P2 buffer with the 30 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... The αM and β2 subunits of recombinant Mac-1 integrin were resolved on 4-20% polyacrylamide (BioRad), gradient gels by SDS-PAGE and stained with Coomassie brilliant blue R250 ...
-
bioRxiv - Microbiology 2023Quote: ... The gel carrying the recombinant proteins and their derivatives was eventually stained with Coomassie Blue (Bio-Rad) for 1 hour and was de-stained with a de-staining solution (20% methanol and 10% acetic acid in water).
-
bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant protein purities were assessed by SDS-PAGE using 4-20% Mini-Protean TGX gels (Bio-Rad, #4561094) and quantified using the BCA Protein Estimation kit (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti-CD8 (Biorad:MCA1226GA) 10 µg ...
-
bioRxiv - Neuroscience 2020Quote: ... Anti-Mouse HRP (BioRad) was used as a secondary detection antibody and immunoblots were developed with Forte HRP substrate (Millipore ...
-
bioRxiv - Immunology 2019Quote: ... and mouse serum (BioRad). Blocking and subsequent surface staining was performed using PBS containing 2 mM EDTA ...