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Citations for Bio-Rad :
451 - 500 of 3233 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... organic acids and glycerol were measured using high-pressure liquid chromatography (HPLC) (LC-2050C, Shimadzu, Kyoto, Japan) equipped with HPX-87H column (Bio-Rad, CA, USA) and a refractive index detector (RID-20A ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2020Quote: ... Gels were rinsed once in water and blotted either with “high molecular weight” or “mixed molecular weight” program of TransBlot® Turbo™ transfer system (Bio-Rad) using Trans-Blot®Turbo™ Midi Nitrocellulose Transfer Packs (170–4159 ...
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bioRxiv - Immunology 2024Quote: ... A total of 1000 ng of RNA was used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit (Bio-Rad, Hercules, CA). Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: 10 μg of protein samples harvested from xenografted human cells were separated in pre-casted 4-15% gradient SDS gels (Biorad, #456-1084) and transferred to nitrocellulose membranes (Millipore #IPVH00010) ...
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bioRxiv - Cell Biology 2020Quote: ... TNF-β were detected by using the premixed 48-plex Bio-Plex Pro Human Cytokine Assay (Bio-Rad, Hercules, CA, USA; 12007283) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: Human cytokines in the serum were measured by Bio-plex Pro TM Human Cytokine Screening 48 plex Bio-PlexTM 200 System (# 12007283, Bio-Rad, US) and Bio-PlexTM 200 System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Human and murine breast cancer cells were washed with PBS and lysed on ice using 1x Laemmli Sample Buffer (Biorad #161-0737) supplemented with protease (Sigma-Aldrich #P8340 ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Immunology 2023Quote: Monitoring of cytokine and chemokine levels was performed using Bio-Plex Pro Human Chemokine 40-plex Panel (Bio-Rad Laboratories, Hercules, California) using a Luminex 200 System ...
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bioRxiv - Immunology 2023Quote: Serum SARS-CoV-2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS-CoV-2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from 200 ng of total RNA purified from high and low heme midguts (using a Bio-Rad iScript cDNA synthesis kit) by priming the reaction with random hexamers to ensure the capture of Sgm derived transcripts ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of high-quality RNA was used for the first-strand cDNA synthesis by using an iScript™ cDNA synthesis kit (Bio-Rad Laboratories, USA). Primer specificity was ensured by performing a BLASTN search of each sequence against the Solanum lycopersicum ITAGv2.40 database (Supplementary Table S1) ...
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bioRxiv - Developmental Biology 2019Quote: ... Gr-1 (1:500, MCA2387, Bio-Rad), Fluorescein labeled DBA-lectin (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were then analysed in duplicate by means of Invitrogen’s Inflammation 20-Plex Human ProcartaPlex™ Panel (#EPX200-12185-901) and read on the Bio-Plex® 200 system (Bio-Rad, 2016). The data is expressed in pg⋅mL−1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Roughly 5 ng of genomic DNA was mixed with primers and probes for a human RPP30 copy number assay labeled with HEX (Bio-Rad, Hercules, CA) as an internal control ...
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bioRxiv - Genetics 2020Quote: ... and was quantified by using a QuantifilerR Human DNA Quantification Kit (Applied Biosystem, USA) on a Bio-Rad Real-time PCR System (Bio-Rad Laboratories, USA). The quantification process was performed per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability dye Viva Fix 498/521 (#1351115) and mouse anti-human CD138 Alexa Fluor 647 (#MCA2459A647) were obtained from Bio-Rad (Hercules, California). CD138 magnetic beads were procured from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad). The trimmed median value was used to derive the standard curve and calculate sample concentrations.
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bioRxiv - Neuroscience 2023Quote: The reference gene used in combination with the designed human PMP22 assay was the commercially established AP3B1 (dHsaCP1000001; #10031245, Bio-Rad, Basel, Switzerland) ddPCR copy number variation assay.
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bioRxiv - Microbiology 2021Quote: ... and cDNA was synthesized from total RNA with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher. Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Biorad; Cat No. 1725121) or SYBR Green PCR master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Lactate concentrations were measured using high-performance liquid chromatograph (Shimadzu, Kyoto, Japan) equipped with an Aminex HPX-87H column (Bio-Rad Laboratories, Inc., Hercules, CA) at the start and the end of each incubation to confirm that the initially added amount of lactate was sufficient to deplete all added terminal electron acceptors (data presented in Table S1).
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bioRxiv - Microbiology 2023Quote: ... Xrn-1 digest samples were diluted 1:1 in 2X RNA loading dye (BioRad) and RNA was denatured at 80°C for 90 seconds ...
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bioRxiv - Microbiology 2020Quote: ... lactate and acetate were measured using a Prominence high-performance liquid chromatograph (Shimadzu, Kyoto, Japan) equipped with an Aminex® HPX-87H column (Bio-Rad Laboratories, Inc., Hercules, CA). Protein concentrations were determined with the Quick Start™ Bradford Protein Assay kit (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... This sequence was amplified from B6J genomic DNA extracted from spleen tissue using iProof High-Fidelity PCR kit (BIO-RAD, Hercules, CA, USA; Cat# 172-5330) with the following primers containing XhoI and HindIII restriction enzyme sites in the sense and antisense primers respectively ...
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bioRxiv - Microbiology 2022Quote: ... and acetate concentrations were measured with a SHIMADZU high performance liquid chromatograph (HPLC) with an Aminex™ HPX-87H Ion Exclusion column (Bio-Rad, 300 mm × 7.8 mm) and an eluent of 8.0 mM sulfuric acid.
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of each of the human plasma samples from the small cohort were diluted in 17 µL of Laemmli sample buffer (Bio-Rad; Hercules, CA, USA) followed by denaturing at 70 °C for 10 min and the resulting denatured 20 µL samples were loaded onto 12% Bis-Tris PAGE gel (GenScript ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:5 (for caecum 1:1 or 1:10) and qPCR was performed on a CFX384 Touch™ (Bio-Rad) with iTaq Universal SYBR (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... and F4/80 (Cl:A3-1, Bio-Rad, 1:1000) staining were performed by the Duke Pathology core (Durham ...
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bioRxiv - Systems Biology 2023Quote: ... F4/80 (Bio-Rad, clone: Cl:A3-1, 1:80), TIM4 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... anti-WC-1 (Bio-Rad, MCA838GA, dilution 1:20) and anti-Pax5 (Agilent DAKO ...
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bioRxiv - Microbiology 2020Quote: ... Levels of the following 27 cytokines were analyzed using a BioPlex Pro™ Human Cytokine 27-plex Assay kit (#M500KCAF0Y, Bio-Rad, Hercules, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... utilizing the bead-based immunoassay approach Bio-Plex Pro™ Human Cytokine 40-plex Assay (Cat# 171AK99MR2) on a Bio-plex 200 system (Cat# 171000201) (Bio-Rad Laboratories, Hercules, CA) and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30 ...
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bioRxiv - Microbiology 2020Quote: ... in oral swabs of positive and negative subjects for SARS-CoV-2 using magnetic bead-based multiplex immunoassays (Bio-Plex Pro™ human cytokine 27-plex panel, Bio-Rad Laboratories, Milan, Italy) according to the pre-optimized protocol 15 ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: Culture medium of HUVECs were collected and analyzed with the Bio-Plex Pro™ Human Cytokine 27-plex Panel (Bio-Rad Laboratories, Hercules, CA, USA) in accordance with the manufacturer’s protocols ...
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bioRxiv - Physiology 2021Quote: ... and mixed 1:1 with 2x Laemmli buffer (Bio-Rad) plus 5% β-mercaptoethanol ...
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bioRxiv - Pathology 2019Quote: ... F4/80 (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100), CD3 (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... The blot was stained with 1:1 enhancer:substrate reagents (BioRad) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
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bioRxiv - Biochemistry 2023Quote: ... The collected cells were mixed 1:1 with TrypanBlue (BioRad) and counted.
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bioRxiv - Genetics 2023Quote: ... 1:500 to 1:1000 anti-rabbit (BioRad, 172-1019). Signal was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... (BioRad Cl:A3-1) and goat anti-rat IgG cross-absorbed Alexa Fluor® 488 secondary antibody (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts (1 mg) of protein were diluted 1:1 in sample loading buffer (2x, Bio-Rad, UK) for each extraction method and loaded onto a 12% Tris-glycine pre-cast SDS gel (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Luminex analyses were performed using the Bio-Plex ProTM Human Cytokine 27-Plex Assay as well as the Bio-Plex Pro™ TGF-β Assay (both Bio-Rad Laboratories, Hercules, USA) according to the manufacturer’s protocols on the Bio-Plex® Reader 200 by using the Bio-Plex Manager™ software.