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Citations for Bio-Rad :
51 - 100 of 2617 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... T7-tagged templates were generated with the iProof High-Fidelity PCR Kit (Bio-Rad) using sequences cloned into plasmids pIB (LacZ) ...
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bioRxiv - Microbiology 2019Quote: SDS-PAGE analysis of the recombinant TMPT2A (rTMPT2A) was performed on linear 12.5% polyacrylamide minigels in a Mini-Protean® Tetra cell system (BioRad, USA), as described by [22] ...
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bioRxiv - Bioengineering 2020Quote: ... The raw data were analyzed and the target molecule concentrations were extracted using the accompanying software (QuantaSoft Software, #1864011, Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2021Quote: ... Recombinant XRCC4-6xHis was immobilised on a GLC chip (BioRad Laboratories) in the vertical orientation ...
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bioRxiv - Immunology 2019Quote: Lung homogenates and a pre-stained recombinant protein ladder (Bio-Rad) were run in a 12% SDS-polyacrylamide gel and were transferred to a nitrocellulose membrane (Bio-Rad) ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Human (Bio-Rad, USA) INSR - PrimePCR™ SYBR® Green Assay ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Human (Bio-Rad, USA). Resultant data was analysed using the proprietary LightCycler® Software 4.1 and Microsoft Excel ...
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bioRxiv - Immunology 2020Quote: ... human CD68 (BioRad MCA5709), human ACE2 (Abcam ab15348) ...
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Expression of negative co-stimulatory molecules together with β-actin and GAPDH endogenous controls were analyzed using CFX96 Touch Real-Time system (BIO-RAD Inc.) in 96-well MicroAmp® optical fast-plates (Applied Biosystems® ...
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bioRxiv - Plant Biology 2023Quote: ... Native protein extracts from E.coli or Synechococcus ΔCS were incubated in IMAC HEPES binding buffer supplemented with potential effector molecules and Ni-charged iminodiacetic acid Profinity™ IMAC Resin (Bio-Rad, USA) on a low speed rotator for 1 h at 23°C ...
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bioRxiv - Biophysics 2022Quote: ... His6-tagged proteins were purified using an Ni-NTA affinity column (Profinity IMAC Ni-Charged, BioRad).
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bioRxiv - Immunology 2020Quote: Human plasma was analysed using the human 27-plex cytokine panel (Bio-Rad) according to manufacturer’s instructions and contained the following targets ...
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bioRxiv - Microbiology 2019Quote: ... were coated with 1 μg/ml recombinant YFV NS1 (Biorad, cat # PIP052A) in 50 mM carbonate buffer (pH ...
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bioRxiv - Neuroscience 2021Quote: ... using known concentrations of recombinant mouse IFN-γ (Bio-Rad, Oxford, UK) and a capturing of anti- IFN-γ mAb ...
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bioRxiv - Immunology 2020Quote: ... These recombinant proteins were incubated with Affi-Prep Polymyxin Media (Bio-Rad) for 4 h at 4 °C to remove endotoxins and endotoxin-bound proteins ...
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bioRxiv - Immunology 2022Quote: ... Generated cDNA was used for real-time qPCR using respective gene targeted intron-spanning gene-specific unlabeled primers and dual-labeled fluorescent FAM probes with a proprietary quencher molecule (PrimePCR™, Bio-Rad, Hercules, CA). Thresholds were set objectively to determine cycle thresholds (CT) ...
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bioRxiv - Plant Biology 2023Quote: ... and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis, Bio-rad). Pichia transformants expressing FHS-CELLOX was already available (Scortica et al. ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then incubated with mouse anti-His antibody (catalog number #6200203, Bio-Rad), diluted 1:1,000 in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated at 370C for a further 24 h with human oxLDL (#5685-3557, Bio-Rad) at a concentration of 0 µg/ml and 25µg/ml ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into CHO cells using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Bioengineering 2020Quote: ... 0.4% β-mercaptoethanol) per lane or 1 μL of strep-tagged unstained protein standards (Bio-Rad # 1610363) and run at 100 V for 2 h in Tris-HCl-Glycine-SDS running buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... human TGFβ1 (PHP143B, Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... human TGFβ1 (PHP143B, Bio-Rad), anti-IL11 antibody (X203 ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCID0017132) and ACTB - PrimePCR™ SYBR® Green Assay ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCED0036269). The cycles to threshold was measured for each well ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCED0044963) INSR - PrimePCR™ SYBR® Green Assay ...
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bioRxiv - Systems Biology 2021Quote: ... Quantitative analyses of blotted hCDKL5 and His-Neuropinilin were carried out with Image Lab software (BioRad) and the volumetric yield was derived considering the final biomass concentration (OD600 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoblots were visualized using horseradish peroxidase–tagged secondary antibody and chemiluminescence (Clarity Western ECL Substrate; BioRad, Hercules, CA). The antibodies used included those directed to lamin A/C ...
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bioRxiv - Neuroscience 2021Quote: ... His6-tagged proteins were then captured from the lysate by Ni2+-IDA chromatography (Profinity IMAC resin, Bio-Rad) under gravity flow and washed with 35mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: Purified γ-TuRC preparations (GCP3- and GCP6-tagged) were resuspended in 20 μL of Laemmli Sample buffer (Biorad) and were loaded on a 4%–12% gradient Criterion XT Bis-Tris precast gel (Biorad) ...
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bioRxiv - Immunology 2021Quote: ... rat anti-human CD8 (Bio-Rad), rat anti-human Granzyme B (eBioscience) ...
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bioRxiv - Genetics 2023Quote: ... Human (Bio-Rad, 10031243, ID: dHsaCP1000002). Reference assay for 3.1 kb deletion and 3.1 kb inversion assays ...
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bioRxiv - Neuroscience 2023Quote: ... anti-human CX3CR1 (Bio-Rad, AHP566). Mouse TNFα ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-human CX3CR1 (Biorad, AHP1589), mouse anti-human CD68 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
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bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Final recombinant protein concentrations were determined using Quick Start Bradford Protein Assays (Bio-Rad) with bovine serum albumin as the reference protein for standard curves ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
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bioRxiv - Microbiology 2024Quote: ... and human proinflammatory cytokines were quantified using the Bio-Plex Pro™ Human Inflammation Panel 1 (BioRad). Fluorescence was read using a MAGPIX System (Luminex ...
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bioRxiv - Cancer Biology 2021Quote: ... 48 cytokines were quantified in the cell supernatant using a Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad, #12007283) according to manufacturer’s protocol ...