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Citations for Bio-Rad :
51 - 100 of 356 citations for Recombinant Human Epidermal Growth Factor Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Transient gene expression in onion epidermal cells was performed using a Biolistic PDS-1000/He Particle Delivery System (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2024Quote: ... PorH-His immunoblots were performed on 16.5% tris-tricine gels (BioRad) using anti-His (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... The signal was normalized with respect to total receptor and quantified using ImageLab software (BioRad).
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bioRxiv - Neuroscience 2022Quote: ... Band density of oxytocin receptor was quantified using Image Lab 6.0 (BioRad Laboratories, Hercules, CA) and normalized to GAPDH.
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bioRxiv - Microbiology 2022Quote: 6His-tagged proteins were affinity-purified using Gravity Flow Chromatography columns (Bio-Rad) loaded with 1-2 ml Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... were transiently expressed in onion epidermal cells after being introduced with a particle gun (PDS-1000He Particle Delivery System, Bio-Rad). The bombarded cells were kept in the dark at 22°C for 16 h followed by GFP imaging using a fluorescent microscope (ECLIPSE 80i ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant plasmid was coated with 0.6 μm gold particles (1 μg DNA per 10 mg gold) and then introduced into onion epidermal cells using the particle bombardment method (PDS-1000/He particle delivery system, Bio-Rad). The epidermis was incubated on MS medium in darkness at room temperature (20–25 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Eight microliters of the micro-particle suspension (30 μl) were used for transformation of epidermal onion cells using the PDE-1000He particle gun (Bio-rad). Before transformation ...
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bioRxiv - Microbiology 2021Quote: ... serial dilutions of recombinant NS1 Antigen (Ag) (Bio-Rad) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... the PrimePCR Estrogen receptor signaling (SAB Target List) H96 panel (Bio-Rad Laboratories, Hercules, CA, USA) was employed for RT-qPCR-based gene array analysis ...
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bioRxiv - Molecular Biology 2021Quote: Cellular growth was evaluated using S3e Cell Sorting equipment (Biorad). Overnight cultures of S ...
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bioRxiv - Plant Biology 2022Quote: ... Imaging was done using Hi-sensitivity chemiluminescence settings under ChemiDoc XRS+ (BioRad).
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bioRxiv - Microbiology 2020Quote: ... T7-tagged templates were generated with the iProof High-Fidelity PCR Kit (Bio-Rad) using sequences cloned into plasmids pIB (LacZ) ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant XRCC4-6xHis was immobilised on a GLC chip (BioRad Laboratories) in the vertical orientation ...
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bioRxiv - Immunology 2019Quote: Lung homogenates and a pre-stained recombinant protein ladder (Bio-Rad) were run in a 12% SDS-polyacrylamide gel and were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Human (Bio-Rad, USA) INSR - PrimePCR™ SYBR® Green Assay ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Human (Bio-Rad, USA). Resultant data was analysed using the proprietary LightCycler® Software 4.1 and Microsoft Excel ...
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bioRxiv - Immunology 2020Quote: ... human CD68 (BioRad MCA5709), human ACE2 (Abcam ab15348) ...
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Invasive growth was measured by the Image Lab 6.0.1 program (Bio-Rad, https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z ...
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bioRxiv - Biophysics 2022Quote: ... His6-tagged proteins were purified using an Ni-NTA affinity column (Profinity IMAC Ni-Charged, BioRad).
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bioRxiv - Immunology 2020Quote: Human plasma was analysed using the human 27-plex cytokine panel (Bio-Rad) according to manufacturer’s instructions and contained the following targets ...
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bioRxiv - Plant Biology 2020Quote: Transient expression of pro35S::VAMP714::GFP and ST–RFP constructs was carried out in onion epidermal peels following microprojectile bombardment using the Helios Gene Gun system (Bio-Rad Laboratories, Hemel Hempstead, UK). Plates containing bombarded onion sections were covered with aluminium foil and incubated at 22° C overnight ...
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bioRxiv - Plant Biology 2022Quote: The GFP and DAI-GFP plasmids were transiently introduced into onion epidermal cells using a helium-driven particle accelerator (PDS/1000; Bio-Rad Laboratories, Hercules, CA, USA). The particle bombardment experiments were performed as described by Arimura et al ...
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bioRxiv - Cell Biology 2019Quote: ... Immunostaining for the vitronectin receptor (VNR) was with anti-CD51/61 (clone 23C6, 1:400; Bio-Rad, Oxford, UK) and for cathepsin K with a rabbit polyclonal antibody (3368-100 ...
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bioRxiv - Microbiology 2019Quote: ... were coated with 1 μg/ml recombinant YFV NS1 (Biorad, cat # PIP052A) in 50 mM carbonate buffer (pH ...
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bioRxiv - Neuroscience 2021Quote: ... using known concentrations of recombinant mouse IFN-γ (Bio-Rad, Oxford, UK) and a capturing of anti- IFN-γ mAb ...
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bioRxiv - Immunology 2020Quote: ... These recombinant proteins were incubated with Affi-Prep Polymyxin Media (Bio-Rad) for 4 h at 4 °C to remove endotoxins and endotoxin-bound proteins ...
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bioRxiv - Plant Biology 2023Quote: ... and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis, Bio-rad). Pichia transformants expressing FHS-CELLOX was already available (Scortica et al. ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then incubated with mouse anti-His antibody (catalog number #6200203, Bio-Rad), diluted 1:1,000 in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... 0.4% β-mercaptoethanol) per lane or 1 μL of strep-tagged unstained protein standards (Bio-Rad # 1610363) and run at 100 V for 2 h in Tris-HCl-Glycine-SDS running buffer ...
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bioRxiv - Cell Biology 2024Quote: Cellular growth was measured every 10 minutes using a microplate reader (680XR Biorad) for OD595 ...
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bioRxiv - Molecular Biology 2022Quote: ... human TGFβ1 (PHP143B, Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... human TGFβ1 (PHP143B, Bio-Rad), anti-IL11 antibody (X203 ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCID0017132) and ACTB - PrimePCR™ SYBR® Green Assay ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCED0036269). The cycles to threshold was measured for each well ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCED0044963) INSR - PrimePCR™ SYBR® Green Assay ...
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bioRxiv - Cell Biology 2023Quote: ... The reaction mixture was separated on SDS-PAGE and the phosphorylated receptor was autoradiographed for image analysis with the Quantity One program (BioRad) on phosphor screens.
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bioRxiv - Biochemistry 2021Quote: ... Immunoblots were visualized using horseradish peroxidase–tagged secondary antibody and chemiluminescence (Clarity Western ECL Substrate; BioRad, Hercules, CA). The antibodies used included those directed to lamin A/C ...
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bioRxiv - Neuroscience 2021Quote: ... His6-tagged proteins were then captured from the lysate by Ni2+-IDA chromatography (Profinity IMAC resin, Bio-Rad) under gravity flow and washed with 35mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: Purified γ-TuRC preparations (GCP3- and GCP6-tagged) were resuspended in 20 μL of Laemmli Sample buffer (Biorad) and were loaded on a 4%–12% gradient Criterion XT Bis-Tris precast gel (Biorad) ...
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bioRxiv - Systems Biology 2021Quote: ... Quantitative analyses of blotted hCDKL5 and His-Neuropinilin were carried out with Image Lab software (BioRad) and the volumetric yield was derived considering the final biomass concentration (OD600 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: ... Plates were oriented vertically in the growth chamber and scanned (GS-800 scanner, BioRad) after 0 ...
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bioRxiv - Physiology 2023Quote: ... taking into account dilution factor (cell count estimated with the BIO-RAD TC20 automated cell counter). Repeatability of the ROS production measurements between sample-duplicates was R = 0.924 (CI 95% = [0.9 ...
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bioRxiv - Cancer Biology 2020Quote: ... spliced IL6 receptor and gp130 expression was assessed by PCR using the MJ Research Peltier Thermal Cycler Tetrad (Bio-Rad, Germany) with the following primers ...
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bioRxiv - Immunology 2021Quote: ... rat anti-human CD8 (Bio-Rad), rat anti-human Granzyme B (eBioscience) ...