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Citations for Bio-Rad :
251 - 300 of 3263 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Molecular Biology 2021Quote: ... The qRT-PCR was performed in a 2-step amplification with 40 cycles of 95 °C for 30 s and 55 °C for 30s using a Bio-Rad IQ thermocycler (Bio-Rad Laboratories Inc. Hercules, CA). Each reaction was loaded on a 384-wells standard qPCR plate and ran in triplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... The enzymes were subsequently incubated at temperatures from 30 °C to 54 °C for one hour using a PCR thermocycler machine (Bio-Rad Laboratories, Hercules, CA, USA), followed by cooling for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Immunology 2021Quote: ... Settings for analysis using a C-1000 CFX96 machine (Bio-Rad) were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... overnight at 37 °C in a G1000TM Thermal cycler (BIO-RAD) to digest trehalose to produce free glucose ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then to 97°C for 8 min in a thermocycler (BioRad). We centrifuged the samples and froze the supernatant for further use.
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-cytochrome c (AHP2302, Bio-Rad Laboratories, 1:500), and mouse monoclonal anti-β-actin (A5441-.2ML ...
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bioRxiv - Microbiology 2019Quote: ... 72°C,20"]x45 using SSO Advanced SYBR Green Supermix (BioRad), and 300 nM Tox9 and 11 primers listed in Table S1 ...
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bioRxiv - Physiology 2023Quote: ... and transferred (100V, 1h, 4°C) onto PVDF membranes (Bio-Rad). Blots were blocked in buffer containing 5% BSA (Cat#A9418 ...
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bioRxiv - Biochemistry 2023Quote: ... 59 and 61°C in an S1000 thermal cycler (Bio-Rad). For CETSA with dose-response ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed using EBC buffer (Bio-Rad Cat. # C-1410) supplemented with protease EDTA-free (Cat # ...
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bioRxiv - Neuroscience 2023Quote: ... and 72°C 15 s using a PCR machine (T100, Biorad). PCR primer pairs used were 5’-TCCCAGCCTGAAAGCCACAG-3’ and 5’-ATGCTGCTGGGATTCTCTCCC-3’ for Pet1 ...
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bioRxiv - Genetics 2023Quote: ... Membranes were incubated overnight at 4°C with STAR5B Streptavidin:HRP (BioRad) at a 1:15,000 dilution in 1x PBST ...
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bioRxiv - Neuroscience 2024Quote: ... the iScript™C gDNA Clear cDNA Synthesis Kit (BIO-RAD) was employed ...
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bioRxiv - Neuroscience 2021Quote: ... and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories, Hercules, CA). Separated proteins were transferred onto nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... For each sample 50 μg of protein were incubated at 95°C for 10 min and then loaded into a gradient (4-15%) western blot gel (mini-protein TGX gel, Bio-Rad, Milan, Italy). Proteins were transferred onto a 0.45 μm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... placed on a heat block (+90 °C) for 10 min and sonicated before separation by SDS-PAGE (4-20% Mini-PROTEAN TGX Gels, Bio-Rad, 456-1096). Next ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 90°C and loaded on a 4–20% (w/v) Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad) ...
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bioRxiv - Physiology 2021Quote: ... The solution with MSP1E3D1 was mixed at 4°C for 10 min before addition of 130 mg of Biobeads SM2 (Bio-Rad, Hercules, CA). Biobeads (washed into methanol ...
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bioRxiv - Biochemistry 2020Quote: Surface biotinylation samples were heated to 50°C for 10 minutes before separation using 4-20% polyacrylamide gradient gels (Bio-Rad, Hercules, CA). After separation ...
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bioRxiv - Biochemistry 2022Quote: REST-FL was purified in two-step chromatography and carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 min at 70°C and then loaded on a 12% Mini-protean TGX gel (Bio-Rad Cat# 4561044). The gels were run for approximately 1 h at 100 V ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... the membranes were washed 3 times for 10 min each with TBST buffer at 24 °C and visualized using enhanced chemiluminescence reagents (Bio-Rad Laboratories, CA, USA). The images were captured using Image Quant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Physiology 2023Quote: Plasma cytokines and chemokines were assessed using a magnetic bead panel (Bio-Rad, Cat. #171K1001M). Plasma samples were diluted 1:4 in assay buffer prior to running the assay according to manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Cytokine/chemokine levels were measured in supernatant of lung homogenate by Bioplex Assay (Bio-rad).
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bioRxiv - Microbiology 2023Quote: ... following manufacturer’s instructions measuring levels of 30 cytokines and chemokines using the BioPlex reader (Biorad). An additional 2-fold dilution was performed on the supplied standards to extend its lower detection limit.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated (4°C, overnight) with 5 % milk powder (BioRad) in TBST ...
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bioRxiv - Neuroscience 2019Quote: ... 72°C for 2’ in a T100™ Thermal Cycler (Bio-Rad) followed by long-term storage at −20°C ...
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bioRxiv - Immunology 2019Quote: ... Cells were analysed at 4°C using a ZE5 Flow cytometer (BioRad) set to sample wells until running dry ...
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bioRxiv - Biophysics 2022Quote: ... followed by 5 min at 60 °C in a thermocycler (Biorad T100) with the lid heated to 105 °C.
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bioRxiv - Biochemistry 2021Quote: ... and electro-blotted onto PVDF membranes (Biorad: 100 V, 2h, 4°C). The transferred proteins were then renatured by progressively reducing the guanidine-HCl concentration ...
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bioRxiv - Immunology 2022Quote: ... before being lysed at 90 °C in a thermal cycler (BioRad T1000) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... permeabilized for 10 min with 0.5% Triton X-100 (Bio-Rad, USA) in phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... Cytokine and chemokine expression were determined using SsoAdvanced Universal SYBR Green Supermix (BIO-RAD catalog: 1725271). 10 μl volume reaction was used with 5 μl SsoAdvanced Universal SYBR Green Supermix(2x) ...
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bioRxiv - Cancer Biology 2024Quote: ... inflammatory cytokines and chemokines were measured using the mouse multiplexed Luminex assays (Bio-Rad, Hercules, CA) according to the manufacturer’s protocols in the University of North Carolina at Chapel Hill (UNC-CH ...
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bioRxiv - Cell Biology 2020Quote: ... samples were heated at 100°C in Laemmli buffer (Bio-Rad, Hercules, CA) and processed for immunoblotting.
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bioRxiv - Plant Biology 2020Quote: ... The gels ran at 4 °C in a Mini-PROTEAN Tetra System (BioRad) at 35 mA per gel for 60 min ...
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bioRxiv - Plant Biology 2020Quote: ... Electrophoresis was performed at 4 °C in a Mini-PROTEAN Tetra System (BioRad) at 100 V for 30 min with Blue Cathode Buffer (15 mM BisTris-HCl ...
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bioRxiv - Bioengineering 2020Quote: ... and heat shocked at 43°C using a thermal cycler (Bio-Rad, 1851148) with various patterns as indicated (Table S2) ...
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bioRxiv - Physiology 2021Quote: ... then 85°C for 5 min on a C1000 thermal cycler (Bio-Rad), then stored at -20°C.
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bioRxiv - Plant Biology 2020Quote: ... for 16 h at 4°C using a wet transfer system from BioRad (transfer buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were blotted overnight at 4°C onto polyvinylidene difluoride (PVDF) membranes (Biorad) and subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... and samples were held at 12°C in the C1000 thermocycler (Bio-Rad) prior to analysis on the droplet reader ...
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bioRxiv - Zoology 2020Quote: ... 58°C for 60s using a CFX96 Real-Time System (BIORAD, Hercules, CA).
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bioRxiv - Microbiology 2021Quote: ... DNA amplification was carried out in a C 1000 Touch™ thermocycler (BIORAD). The reference gDNA samples of P ...