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Citations for Bio-Rad :
151 - 163 of 163 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: SDS-PAGE analysis of the recombinant TMPT2A (rTMPT2A) was performed on linear 12.5% polyacrylamide minigels in a Mini-Protean® Tetra cell system (BioRad, USA), as described by [22] ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... and the fractions containing the recombinant vaccine candidates were automatically collected using BioFrac™ Fraction Collector (Bio-Rad Laboratories, Hercules, USA) when the 280 nm absorbance was greater than 0.05 ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Bioengineering 2023Quote: ... a constant voltage of was applied (200-300 V to obtain E = 40-60 V/cm) using a DC power supply (Bio-Rad PowerPac Basic, Hercules, CA). At PAGE completion ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: The recombinant protein of each vaccine candidate was purified under denaturing conditions using a biologic duoflow™ chromatography system (Bio-Rad Laboratories, Hercules, USA). A HisTrap™ HP column (GE Healthcare Life Sciences ...