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Citations for Bio-Rad :
501 - 550 of 6588 citations for Rat Vasoactive Intestinal Polypeptide Receptor 1 VIPR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: The isolated total RNA (1 μg) was reverse-transcribed to produce cDNA using iScript Reverse Transcription Supermix kit (Bio-Rad). Real-time PCR was performed using SYBR Green supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Up to 1 µg of total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) or gDNA Clear iScript cDNA Synthesis Kit for patient-derived tumor cells (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µg of RNA from each sample was reverse-transcribed in 40 µl using iScript™ cDNA Synthesis Kit (BioRad).
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bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Neuroscience 2019Quote: ... conjugated secondary antibody (1:10 000) was subsequently applied and visualized by reaction with chemiluminesence reagent (Clarity Western Blotting Substrates Kit, BioRad) on radiography film (Carestream Blue X-ray film).
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using 1 µg of total RNA and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). Synthesized cDNAs were then quantified by real time quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2022Quote: 1 μg of total RNA purified from differentiated cells was reversed transcribed using the One-step SuperScript kit (Bio-Rad). The qRT-PCR reaction was performed in a CFX96 Real-time PCR detection system (Bio-Rad ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Molecular Biology 2022Quote: ... the pellets were re-dissolved in 1 M NaOH and quantified by Bradford assay (Quick StartTM Bradford Kit, BIO-RAD), and the supernatants containing cADPR were neutralized with Chloroform/Tri-n-octylamine mixture (volume ratio 3:1 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of total RNA were treated with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; https://www.bio-rad.com/) for genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 1 µg RNA was used for cDNA synthesis with the IScript cDNA Synthesis Kit (Bio-Rad, 1708890), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... We then utilized 1 µg of the total RNA for the SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad). rRNA depletion was performed with riboPOOL for D ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from total purified RNA (1 μg) from each sample by using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... After incubation with HRP-coupled secondary antibody diluted in saturation buffer (1 h, room temperature), membranes were revelated using ECL kit (Pierce, 34076) on Chemidoc XRS+ imager (Biorad). Images were processed and analyzed using ImageJ software ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Physiology 2024Quote: ... Total RNA (1 μg) was reverse transcribed to cDNA using an iScript cDNA synthesis kit (Cat no. 170884, BioRad, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
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bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions (Clontech ...
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bioRxiv - Microbiology 2019Quote: ... 39) and 1 µg of total RNA was reverse transcribed to cDNA using the iScript Select cDNA Synthesis Kit (Bio-Rad) with random hexamers ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription of 1 μg of RNA in a total volume of 20 μl was performed with iScript cDNA Synthesis Kit (Bio-Rad) prior to real time PCR (iCycler ...
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bioRxiv - Physiology 2020Quote: ... We synthesized a total of 500 ng (pituitary) and 1 μg (gonad) of RNA into cDNA using the iScript cDNA synthesis kit (Bio-Rad) in 20 μl reactions according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed cDNA synthesis with 0.5-1 μg of total RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed Real Time quantitative PCR (RT-qPCR ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Immunology 2020Quote: Total RNA (up to 1 µg per reaction) from BMDM was reverse transcribed with iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative PCR was performed using 1 µl of cDNA ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the RNA was assessed and first strand cDNA synthesised from 1 ug using the iScript Select cDNA Synthesis Kit (Bio-Rad) and a 1:1 mixture of Oligo (dT ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2022Quote: ... RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad). Primers for five genes—Clock ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 µg RNA (sorted cells) or 1 µg RNA (tissues) was used to prepare cDNA with the iScript™ cDNA synthesis kit (Biorad) with the following protocol ...
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bioRxiv - Neuroscience 2023Quote: ... analysis was performed by reverse transcribing 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). iQ quantitative PCR was performed on the diluted cDNA following the manufacturer’s protocol (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: cDNA was synthesized by conversion of 1 µg of the DNAse-I treated RNA with iScript Selected cDNA Synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... diluted to 1 ng/μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 1 μg of total RNA using the Reverse Transcription system (iScriptTM cDNA synthesis kit, Bio-Rad). Quantitative Real time PCR was performed using Sybr Green Master Mix (SYBR Green master mix ...
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bioRxiv - Systems Biology 2023Quote: We performed cDNA synthesis with 0.5-1 μg of total purified RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with QIAquick® PCR purification kit and 1-10 ng of immunoprecipitated or input DNA were amplified with iQ™ SYBR Green Supermix (BioRad) using a Real-Time PCR machine (BioRad IQ™ 5) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...