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Citations for Bio-Rad :
451 - 500 of 4640 citations for Rat 3 Hydroxy 3 Methylglutaryl CoA Reductase HMGCR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1hr on ice with varying concentrations of fluorescently labeled hybridoma-derived or recombinant mAbs and then washed x3 in flow buffer (PBS + 3% fetal calf serum) prior to analysis on a ZE5 flow cytometer (BioRad, Hercules, CA). Equilibrium binding affinities were calculated via nonlinear regression (One site – total and non-specific binding ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...
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bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml Tween20 add to 1000 ml with ddH2O) 3 times for 20 min and blocked with 10% dried milk-TBST solution or BlockAce-TBST solution (BlockAce, Bio-Rad, #170223) for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
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bioRxiv - Developmental Biology 2019Quote: Oocytes were collected in 0.1% PVP in DPBS and boiled for 3 mins at 95°C in 1x Laemmli Sample Buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... (see Supplementary Table 3 for a full list of oligos used in the present study) into 384-well hard-shell PCR plates (Bio-Rad HSP3901) using Mantis liquid handler (Formulatrix) ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated to 50°C for 2 to 3 min before the addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexed vigorously for 5 sec before pipetting in plug mould (Bio-Rad 1703713) ...
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bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; Bio-Rad, 170-6516) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Systems Biology 2022Quote: ... rat anti-F4/80 (BioRad, MCA497R, 1:100) or rabbit anti-CYP2E1 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-Cd68 (1:1000, AbDserotec-BioRad, MCA1957); rabbit anti-Iba-1 (1:800 ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-CD68 (1:500, MCA1957, Bio-Rad) and mouse anti-PSD-95 (1:500 ...
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bioRxiv - Pathology 2022Quote: ... Rat anti Dectin2 (1:200, Bio-Rad, MCA2415T), and Goat anti-CXCL10(1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-alpha Tubulin (1:500 Bio-Rad), rabbit anti-NudE/NudEL antibody (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... and rat IgG and visualized using chemiluminescence (Biorad) and an ImageQuant LAS 4000 scanner (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-alpha tubulin (1:1000, Bio-Rad). All primary antibodies were incubated overnight at 4°C with shaking ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-rat CD2 (1:100, MCA154GA, Biorad); mouse anti hts (1:200 ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: ... CD68 (Rat monoclonal, 1:250, BioRad, Cat# MCA1957), Homer1 (Rabbit polyclonal ...
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bioRxiv - Neuroscience 2021Quote: ... and BrdU (Rat anti-BrdU, Biorad, 1:500) incubated at 4°C overnight and followed by incubation of 488- and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2021Quote: ... and rat anti-MBP (MCA409, Biorad, 1:500) in 10 % goat serum ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-rat CD68 (clone ED1; Bio-Rad), and mouse anti-β-tubulin (clone TUB 2.1 ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-CD68 (1:500, Bio-Rad; MCA1957), or rabbit anti-FOSB (1:500 ...
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bioRxiv - Pathology 2022Quote: ... rat anti-F4/80 (Biorad, MCA97R, 1:200), recombinant anti-Cre (Merck Millipore ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... rat anti-CD68 (1:100; Bio-Rad, MCA1957GA), mouse anti-MBP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-rat CD2 (Bio-Rad, 1:200), rat anti-mCD8 (Caltag ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-CD3 (1:50; #MCA1477 Bio-Rad), rat anti-CD45R (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-CD68 (1:1000, MCA1957; Biorad, USA); rabbit anti-Cd74 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-CD68 (1:500, Bio-Rad, MCA1957), rabbit anti-PSD95 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The corresponding rat anti-mouse (Biorad; Cat# 1706516) or goat anti-rabbit (Biorad ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-mouse F4/80 (MCA497, BIO-RAD); 1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary goat anti-rat Horseradish Peroxidase (HRP; Biorad) was used ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-CD206 (1:250, Bio-Rad, MCA2235), mouse anti-NeuN (1:500 ...